caa a22 4500 445880880 CHVBK 20180317145542.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s00604-011-0547-x doi (NATIONALLICENCE)springer-10.1007/s00604-011-0547-x Interaction of polymyxin B with ds-DNA, and determination of DNA or polymyxin B via resonance Rayleigh scattering and resonance non-linear scattering spectra [Elektronische Daten] [Ling Kong, Zhongfang Liu, Xiaoli Hu, Shaopu Liu] Assays were developed for DNA or polymyxin B (PMB) based on enhanced resonance Rayleigh scattering (RRS) and resonance nonlinear scattering (including second order scattering and frequency doubling scattering) that result from the interaction of PMB with DNA. A minor-groove binding mechanism is suggested from the results obtained with RRS and from absorption and circular dichroism spectroscopy. The types of interaction and reasons of RRS enhancement are discussed. Linear relationships do exist over a wide range between the intensity of enhanced scattering and the concentrations of either DNA or PMB. When ctDNA is used as a probe to determine PMB, the detection limit (3σ) is 9.8ng mL−1. When PMB is used as a probe to determine DNA, the detection limit (3σ) is in the range from 3.8 to 9.0ng mL−1. Figure Assays were developed for DNA or polymyxin B (PMB) based on enhanced resonance Rayleigh scattering (RRS) and resonance nonlinear scattering (including second order scattering and frequency doubling scattering) that result from the interaction of PMB with DNA. A minor-groove binding mechanism is suggested from the results obtained with RRS and from absorption and circular dichroism spectroscopy. The types of interaction and reasons of RRS enhancement are discussed. Linear relationships do exist over a wide range between the intensity of enhanced scattering and the concentrations of either DNA or PMB. When ctDNA is used as a probe to determine PMB, the detection limit (3σ) is 9.8 ng mL−1. When PMB is used as a probe to determine DNA, the detection limit (3σ) is in the range from 3.8 to 9.0 ng mL−1 Springer-Verlag, 2011 Polymyxin B nationallicence DNA nationallicence Resonance Rayleigh scattering nationallicence Resonance nonlinear scattering nationallicence Kong Ling School of Chemistry and Chemical Engineering, Key Laboratory of Luminescence and Real-time Analysis of the Ministry of Education, Southwest University, 400715, Chongqing, China aut Liu Zhongfang School of Chemistry and Chemical Engineering, Key Laboratory of Luminescence and Real-time Analysis of the Ministry of Education, Southwest University, 400715, Chongqing, China aut Hu Xiaoli School of Chemistry and Chemical Engineering, Key Laboratory of Luminescence and Real-time Analysis of the Ministry of Education, Southwest University, 400715, Chongqing, China aut Liu Shaopu School of Chemistry and Chemical Engineering, Key Laboratory of Luminescence and Real-time Analysis of the Ministry of Education, Southwest University, 400715, Chongqing, China aut Microchimica Acta Springer Vienna 173/1-2(2011-04-01), 207-213 0026-3672 173:1-2<207 2011 173 604 https://doi.org/10.1007/s00604-011-0547-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00604-011-0547-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kong Ling School of Chemistry and Chemical Engineering, Key Laboratory of Luminescence and Real-time Analysis of the Ministry of Education, Southwest University, 400715, Chongqing, China aut NATIONALLICENCE 700 1- Liu Zhongfang School of Chemistry and Chemical Engineering, Key Laboratory of Luminescence and Real-time Analysis of the Ministry of Education, Southwest University, 400715, Chongqing, China aut NATIONALLICENCE 700 1- Hu Xiaoli School of Chemistry and Chemical Engineering, Key Laboratory of Luminescence and Real-time Analysis of the Ministry of Education, Southwest University, 400715, Chongqing, China aut NATIONALLICENCE 700 1- Liu Shaopu School of Chemistry and Chemical Engineering, Key Laboratory of Luminescence and Real-time Analysis of the Ministry of Education, Southwest University, 400715, Chongqing, China aut NATIONALLICENCE 773 0- Microchimica Acta Springer Vienna 173/1-2(2011-04-01), 207-213 0026-3672 173:1-2<207 2011 173 604 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer