caa a22 4500 445880945 CHVBK 20180317145543.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s00604-010-0536-5 doi (NATIONALLICENCE)springer-10.1007/s00604-010-0536-5 Biosensor for L-phenylalanine based on the optical detection of NADH using a UV light emitting diode [Elektronische Daten] [Takahiro Arakawa, Tomoyuki Koshida, Tomoko Gessei, Kumiko Miyajima, Daishi Takahashi, Hiroyuki Kudo, Kazuyoshi Yano, Kohji Mitsubayashi] A biosensor was developed for the detection of L-phenylalanine (Phe) and demonstrated for use in the diagnosis of phenylketonuria (PKU). It consists of L-phenylalanine dehydrogenase (L-PheDH) immobilized on a membrane, an ultraviolet light-emitting diode excitation system, and a photomultiplier tube. The L-PheDH was immobilized on a teflon membrane modified with 2-methacryloyloxyethyl phosphorylcholine and placed at the distal and of an optical fiber. The concentration of Phe was determined by immersing the sensor into a sample solution that also contained NAD+ and measurement of the fluorescence of the NADH produced by enzymatic reaction. Two L-PheDHs (from Thermoactinomyces intermedius and Sporosarcina sp.) were studied and compared. The fluorescence intensities of the biosensor are linearly related to the L-Phe concentrations in the range from 10 μmolL−1 to 10 mmolL−1. The sensor also was operated in the kinetic mode by differential determination of the slope of the signal within 2 min. The analytical range of the sensor is adequate for application in the genotypic diagnosis of PKU (diagnostic value >600 μmolL−1). High sensitivity, good cost-benefit ratio, and low power consumption are typical features of this biosensing system that can can be applied to routine screening of newborn. Figure Nicotinamide adenine dinucleotide (NADH) detecting biosensor of L-phenylalanine (Phe) was developed and demonstrated for diagnosis of phenylketonuria. This biosensor was comprised of L-phenylalanine dehydrogenase immobilized membrane, an ultra violet light-emitting diodes excitation system and a photomultiplier tube. The sensor was sufficient to clinical application of genotypic diagnosis for phenylketonuria. Springer-Verlag, 2010 Optical biosensor nationallicence L-phenylalanine nationallicence Ultraviolet light-emitting diodes excitation nationallicence Phenylalanine dehydrogenase nationallicence NADH nationallicence Arakawa Takahiro Department of Biomedical Devices and Instrumentation, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda-ku, 101-0062, Tokyo, Japan aut Koshida Tomoyuki Graduate school of Bionics, Computer and Media Science, Tokyo University of Technology, 1404-1 Katakura, Hachioji, 192-0982, Tokyo, Japan aut Gessei Tomoko Department of Biomedical Devices and Instrumentation, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda-ku, 101-0062, Tokyo, Japan aut Miyajima Kumiko Department of Biomedical Devices and Instrumentation, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda-ku, 101-0062, Tokyo, Japan aut Takahashi Daishi Department of Biomedical Devices and Instrumentation, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda-ku, 101-0062, Tokyo, Japan aut Kudo Hiroyuki Department of Biomedical Devices and Instrumentation, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda-ku, 101-0062, Tokyo, Japan aut Yano Kazuyoshi Graduate school of Bionics, Computer and Media Science, Tokyo University of Technology, 1404-1 Katakura, Hachioji, 192-0982, Tokyo, Japan aut Mitsubayashi Kohji Department of Biomedical Devices and Instrumentation, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda-ku, 101-0062, Tokyo, Japan aut Microchimica Acta Springer Vienna 173/1-2(2011-04-01), 199-205 0026-3672 173:1-2<199 2011 173 604 https://doi.org/10.1007/s00604-010-0536-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00604-010-0536-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Arakawa Takahiro Department of Biomedical Devices and Instrumentation, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda-ku, 101-0062, Tokyo, Japan aut NATIONALLICENCE 700 1- Koshida Tomoyuki Graduate school of Bionics, Computer and Media Science, Tokyo University of Technology, 1404-1 Katakura, Hachioji, 192-0982, Tokyo, Japan aut NATIONALLICENCE 700 1- Gessei Tomoko Department of Biomedical Devices and Instrumentation, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda-ku, 101-0062, Tokyo, Japan aut NATIONALLICENCE 700 1- Miyajima Kumiko Department of Biomedical Devices and Instrumentation, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda-ku, 101-0062, Tokyo, Japan aut NATIONALLICENCE 700 1- Takahashi Daishi Department of Biomedical Devices and Instrumentation, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda-ku, 101-0062, Tokyo, Japan aut NATIONALLICENCE 700 1- Kudo Hiroyuki Department of Biomedical Devices and Instrumentation, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda-ku, 101-0062, Tokyo, Japan aut NATIONALLICENCE 700 1- Yano Kazuyoshi Graduate school of Bionics, Computer and Media Science, Tokyo University of Technology, 1404-1 Katakura, Hachioji, 192-0982, Tokyo, Japan aut NATIONALLICENCE 700 1- Mitsubayashi Kohji Department of Biomedical Devices and Instrumentation, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda-ku, 101-0062, Tokyo, Japan aut NATIONALLICENCE 773 0- Microchimica Acta Springer Vienna 173/1-2(2011-04-01), 199-205 0026-3672 173:1-2<199 2011 173 604 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer