caa a22 4500 445881011 CHVBK 20180317145543.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s00604-011-0555-x doi (NATIONALLICENCE)springer-10.1007/s00604-011-0555-x Method for determination of microcystin-leucine-arginine in water samples based on the quenching of the fluorescence of bioconjugates between CdSe/CdS quantum dots and microcystin-leucine-arginine antibody [Elektronische Daten] [Xiaopan Zhou, Yuanhua Meng, Hongbing Ma, Guanhong Tao] We have developed a method for the determination of microcystin-leucine-arginine (MC-LR) in water samples that is based on the quenching of the fluorescence of bioconjugates between CdSe/CdS quantum dots (QDs) and the respective antibody after binding of MC-LR. The core-shell CdSe/CdS QDs were modified with 2-mercaptoacetic acid to improve water solubility while their high quantum yields were preserved. Monoclonal MC-LR antibody was then covalently bioconjugated to the QDs. It was found that the fluorescence intensity of the bioconjugates was quenched in the presence of MC-LR. A linear relationship exists between the extent of quenching and the concentration of MC-LR. Parameters affecting the quenching were investigated and optimized. The limit of detection is 6.9 × 10−11molL−1 (3σ). The method was successfully applied to the determination of MC-LR in water samples. Figure Bioconjugates of CdSe/CdS quantum dots and anti-microcystin-leucine-arginine (MC-LR) antibody were prepared through step A to C. Their fluorescence intensity was quenched linearly with addition of MC-LR at different concentrations (step D). A method for determination of MC-LR was thus established and it was simple, sensitive and specific with low-cost instrumentation Springer-Verlag, 2011 Quantum dots nationallicence Bioconjugates nationallicence Microcystin nationallicence Fluorescence nationallicence Water samples nationallicence Zhou Xiaopan College of Chemistry, Chemical Engineering and Materials Science, Soochow University, 215123, Suzhou, Jiangsu, People's Republic of China aut Meng Yuanhua College of Chemistry, Chemical Engineering and Materials Science, Soochow University, 215123, Suzhou, Jiangsu, People's Republic of China aut Ma Hongbing College of Life Science, Soochow University, 215123, Suzhou, Jiangsu, People's Republic of China aut Tao Guanhong College of Chemistry, Chemical Engineering and Materials Science, Soochow University, 215123, Suzhou, Jiangsu, People's Republic of China aut Microchimica Acta Springer Vienna 173/1-2(2011-04-01), 259-266 0026-3672 173:1-2<259 2011 173 604 https://doi.org/10.1007/s00604-011-0555-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00604-011-0555-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhou Xiaopan College of Chemistry, Chemical Engineering and Materials Science, Soochow University, 215123, Suzhou, Jiangsu, People's Republic of China aut NATIONALLICENCE 700 1- Meng Yuanhua College of Chemistry, Chemical Engineering and Materials Science, Soochow University, 215123, Suzhou, Jiangsu, People's Republic of China aut NATIONALLICENCE 700 1- Ma Hongbing College of Life Science, Soochow University, 215123, Suzhou, Jiangsu, People's Republic of China aut NATIONALLICENCE 700 1- Tao Guanhong College of Chemistry, Chemical Engineering and Materials Science, Soochow University, 215123, Suzhou, Jiangsu, People's Republic of China aut NATIONALLICENCE 773 0- Microchimica Acta Springer Vienna 173/1-2(2011-04-01), 259-266 0026-3672 173:1-2<259 2011 173 604 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer