caa a22 4500 445881046 CHVBK 20180317145543.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s00604-011-0548-9 doi (NATIONALLICENCE)springer-10.1007/s00604-011-0548-9 Simultaneous determination of four different antibiotic residues in honey by chemiluminescence multianalyte chip immunoassays [Elektronische Daten] [Klaus Wutz, Reinhard Niessner, Michael Seidel] We are presenting the first method for identification and quantification of antibiotic derivatives in honey samples using regenerable antigen microarrays in combination with an automated flow injection system. The scheme is based on an indirect competitive immunoassay format using monoclonal antibodies bound to the surface of the microarray. The surface of glass slides was coated with epoxy-activated poly(ethylene glycol) and enables direct immobilization of the antibiotic derivatives. The antigen/antibody interaction on the surface of the chip can be detected by chemiluminescence (CL) read-out via CCD camera. The method allows for fast analysis of the four analytes simultaneously and without purification or extraction. An effective data evaluation method also was developed to warrant unambiguous identification of the spots and to establish grey levels of CL intensities. The software developed enables fast and automated processing of the CL images. Dose-response curves were obtained for the derivatives of enrofloxacin, sulfadiazine, sulfamethazine and streptomycin. Spiking experiments revealed adequate recoveries within the dynamic ranges of the calibration curves of enrofloxacin (92% ± 6%), sulfamethazine (130% ± 21%), sulfadiazine (89% ± 20%) and streptomycin (93% ± 4%). Figure Flow-scheme of the chemiluminescence multianalyte chip immunoassay for the determination of antibiotic residues in honey Springer-Verlag, 2011 Antibiotic microarray nationallicence Chemiluminescence detection nationallicence Regenerable biochip nationallicence Automated flow-injection system nationallicence Microarray image evaluation nationallicence Wutz Klaus Institute of Hydrochemistry and Chair for Analytical Chemistry, Technische Universität München, Marchioninistraße 17, 81377, München, Germany aut Niessner Reinhard Institute of Hydrochemistry and Chair for Analytical Chemistry, Technische Universität München, Marchioninistraße 17, 81377, München, Germany aut Seidel Michael Institute of Hydrochemistry and Chair for Analytical Chemistry, Technische Universität München, Marchioninistraße 17, 81377, München, Germany aut Microchimica Acta Springer Vienna 173/1-2(2011-04-01), 1-9 0026-3672 173:1-2<1 2011 173 604 https://doi.org/10.1007/s00604-011-0548-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00604-011-0548-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wutz Klaus Institute of Hydrochemistry and Chair for Analytical Chemistry, Technische Universität München, Marchioninistraße 17, 81377, München, Germany aut NATIONALLICENCE 700 1- Niessner Reinhard Institute of Hydrochemistry and Chair for Analytical Chemistry, Technische Universität München, Marchioninistraße 17, 81377, München, Germany aut NATIONALLICENCE 700 1- Seidel Michael Institute of Hydrochemistry and Chair for Analytical Chemistry, Technische Universität München, Marchioninistraße 17, 81377, München, Germany aut NATIONALLICENCE 773 0- Microchimica Acta Springer Vienna 173/1-2(2011-04-01), 1-9 0026-3672 173:1-2<1 2011 173 604 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer