caa a22 4500 445881186 CHVBK 20180317145544.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s00604-011-0642-z doi (NATIONALLICENCE)springer-10.1007/s00604-011-0642-z Determination of thiol compounds by solid-phase extraction using multi-walled carbon nanotubes as adsorbent coupled with high-performance liquid chromatography-fluorescence detection [Elektronische Daten] [Ke-Jing Huang, Cong-Hui Han, Chao-Qun Han, Jing Li, Zhi-Wei Wu, Yan-Ming Liu] We describe a method for solid-phase extraction of biogenic thiols using multi-walled carbon nanotubes as adsorbent, and their subsequent determination via HPLC and fluorescence detection. The fluorogenic reagent N-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-yl)methyl) iodoacetamide was applied to derivatizate the thiols. The type of eluent and its volume, the sample pH, extraction time and sample volume were optimized. The calibration curves of the thiols are linear in the range from 0.5 to 200nM (for glutathione), 0.02 to 5nM (for cysteine), and 2 to 500nM (for acetylcysteine), and the correlation coefficients range between 0.9955 and 0.9997. The respective limits of detection are 20pM, 4pM and 80pM (at an SNR of 3), and the limits of quatification are 67pM, 13pM, and 267pM (at an SNR of 10). Recoveries range from 85.0% to 113.1% for human urine and plasma samples spiked with the three thiols, and relative standard deviations are in the range from 2.1 to 7.4%. Figure Chromatograms for thiols derivatives obtained by MWCNTs-based SPE-HPLC (A) and direct HPLC analysis (B). Springer-Verlag, 2011 High-performance liquid chromatography-fluorescence detection nationallicence Solid-phase extraction nationallicence Multi-walled carbon nanotubes nationallicence Thiol compounds nationallicence Huang Ke-Jing College of Chemistry and Chemical Engineering, Xinyang Normal University, 464000, Xinyang, People's Republic of China aut Han Cong-Hui College of Chemistry and Chemical Engineering, Xinyang Normal University, 464000, Xinyang, People's Republic of China aut Han Chao-Qun College of Chemistry and Chemical Engineering, Xuchang University, 461000, Xuchang, People's Republic of China aut Li Jing College of Chemistry and Chemical Engineering, Xinyang Normal University, 464000, Xinyang, People's Republic of China aut Wu Zhi-Wei College of Chemistry and Chemical Engineering, Xinyang Normal University, 464000, Xinyang, People's Republic of China aut Liu Yan-Ming College of Chemistry and Chemical Engineering, Xinyang Normal University, 464000, Xinyang, People's Republic of China aut Microchimica Acta Springer Vienna 174/3-4(2011-09-01), 421-427 0026-3672 174:3-4<421 2011 174 604 https://doi.org/10.1007/s00604-011-0642-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00604-011-0642-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Huang Ke-Jing College of Chemistry and Chemical Engineering, Xinyang Normal University, 464000, Xinyang, People's Republic of China aut NATIONALLICENCE 700 1- Han Cong-Hui College of Chemistry and Chemical Engineering, Xinyang Normal University, 464000, Xinyang, People's Republic of China aut NATIONALLICENCE 700 1- Han Chao-Qun College of Chemistry and Chemical Engineering, Xuchang University, 461000, Xuchang, People's Republic of China aut NATIONALLICENCE 700 1- Li Jing College of Chemistry and Chemical Engineering, Xinyang Normal University, 464000, Xinyang, People's Republic of China aut NATIONALLICENCE 700 1- Wu Zhi-Wei College of Chemistry and Chemical Engineering, Xinyang Normal University, 464000, Xinyang, People's Republic of China aut NATIONALLICENCE 700 1- Liu Yan-Ming College of Chemistry and Chemical Engineering, Xinyang Normal University, 464000, Xinyang, People's Republic of China aut NATIONALLICENCE 773 0- Microchimica Acta Springer Vienna 174/3-4(2011-09-01), 421-427 0026-3672 174:3-4<421 2011 174 604 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer