caa a22 4500 445881321 CHVBK 20180317145544.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s00604-011-0617-0 doi (NATIONALLICENCE)springer-10.1007/s00604-011-0617-0 Direct electrochemistry of myoglobin in a layer-by-layer film on an ionic liquid modified electrode containing CeO2 nanoparticles and hyaluronic acid [Elektronische Daten] [Ruifang Gao, Jianbin Zheng, Xiaohui Zheng] We describe an ionic liquid modified electrode (CPE-IL) for sensing hydrogen peroxide (HP) that was modified by the layer-by-layer technique with myoglobin (Mb). In addition, the surface of the electrode was modified with CeO2 nanoparticles (nano-CeO2) and hyaluronic acid. UV-vis and FTIR spectroscopy confirmed that Mb retains its native structure in the composite film. Scanning electron microscopy showed that the nano-CeO2 closely interact with Mb to form an inhomogeneously distributed film. Cyclic voltammetry reveals a pair of quasi-reversible redox peaks of Mb, with the cathodic peak at −0.357V and the anodic peak at −0.269V. The peak separation (ΔE p) and the formal potential (E 0′) are 88mV and −0.313V (vs. Ag/AgCl), respectively. The Mb immobilized in the modified electrode displays an excellent electrocatalytic activity towards HP in the 0.6 to 78.0μM concentration range. The limit of detection is 50nM (S/N = 3), and then the Michaelis-Menten constant is 71.8μM. We believe that such a composite film has potential to further investigate other redox proteins and in the fabrication of third-generation biosensors. Figure The HA/CeO2/Mb/CPE-IL displayed a pair of quasi-reversible redox peaks. The cathodic peak and the anodic peak of Mb were observed at −0.357V and −0.269V with the formal potential (E 0′) of −0.313V and the ΔE p was decreased to 88mV (curve f). Springer-Verlag, 2011 Electrochemistry nationallicence Direct electron transfer nationallicence Ionic liquid nationallicence Myoglobin nationallicence Hydrogen peroxide nationallicence Gao Ruifang Institute of Analytical Science, Shaanxi Provincial Key Laboratory of Electroanalytical Chemistry, Northwest University, 710069, Xi'an, Shaanxi, People's Republic of China aut Zheng Jianbin Institute of Analytical Science, Shaanxi Provincial Key Laboratory of Electroanalytical Chemistry, Northwest University, 710069, Xi'an, Shaanxi, People's Republic of China aut Zheng Xiaohui Institute of Analytical Science, Shaanxi Provincial Key Laboratory of Electroanalytical Chemistry, Northwest University, 710069, Xi'an, Shaanxi, People's Republic of China aut Microchimica Acta Springer Vienna 174/3-4(2011-09-01), 273-280 0026-3672 174:3-4<273 2011 174 604 https://doi.org/10.1007/s00604-011-0617-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00604-011-0617-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gao Ruifang Institute of Analytical Science, Shaanxi Provincial Key Laboratory of Electroanalytical Chemistry, Northwest University, 710069, Xi'an, Shaanxi, People's Republic of China aut NATIONALLICENCE 700 1- Zheng Jianbin Institute of Analytical Science, Shaanxi Provincial Key Laboratory of Electroanalytical Chemistry, Northwest University, 710069, Xi'an, Shaanxi, People's Republic of China aut NATIONALLICENCE 700 1- Zheng Xiaohui Institute of Analytical Science, Shaanxi Provincial Key Laboratory of Electroanalytical Chemistry, Northwest University, 710069, Xi'an, Shaanxi, People's Republic of China aut NATIONALLICENCE 773 0- Microchimica Acta Springer Vienna 174/3-4(2011-09-01), 273-280 0026-3672 174:3-4<273 2011 174 604 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer