caa a22 4500 445881356 CHVBK 20180317145544.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s00604-011-0634-z doi (NATIONALLICENCE)springer-10.1007/s00604-011-0634-z Chemiluminescence assay for angiogenin using a signal amplification technology based on the cleavage of nicking endonucleases [Elektronische Daten] [Yunhua He, Feng Nie] Traditional protein assays based on the recognition of a target molecule by signal probe in a 1:1 stoichiometric ratio are limitated in sensitivity because one target molecule causes the production of a single signal probe only. We report on a novel approach for the determination of angiogenin (Ang). It is based on the cleavage of the Nb.Bsm I (nicking endonucleases) and an associated signal amplification technology using Au nanoparticles (AuNPs) as labels. The signal is amplified via Ang-aptamer recognition and after cleavage of the Nb.Bsm I, upon which one Ang can produce several chemiluminescent signal probes based on AuNPs. This results in a significant increase in the limit of detection of Ang (0.2pmol L−1). The method also is highly selective. Ang was determined in serum samples, and the results confirmed the reliability and practicality of the protocol. Figure A novel approach for the determination of angiogenin (Ang) was developed based on the cleavage of the Nb.Bsm I and an associated signal amplification technology using Au nanoparticles (AuNPs) as labels. This results in a significant increase in the limit of detection of Ang (0.2pmol L−1) Springer-Verlag, 2011 Nb.BsmI nationallicence Chemiluminescence nationallicence Angiogenin nationallicence Signal amplification nationallicence Au nanoparticles nationallicence He Yunhua School of Environmental Science and Engineering, Chang'an University, 710054, Xi'an, China aut Nie Feng School of Chemical and Enviromental Sciences, Shaanxi University of Technology, 723001, Hanzhong, China aut Microchimica Acta Springer Vienna 174/3-4(2011-09-01), 375-382 0026-3672 174:3-4<375 2011 174 604 https://doi.org/10.1007/s00604-011-0634-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00604-011-0634-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- He Yunhua School of Environmental Science and Engineering, Chang'an University, 710054, Xi'an, China aut NATIONALLICENCE 700 1- Nie Feng School of Chemical and Enviromental Sciences, Shaanxi University of Technology, 723001, Hanzhong, China aut NATIONALLICENCE 773 0- Microchimica Acta Springer Vienna 174/3-4(2011-09-01), 375-382 0026-3672 174:3-4<375 2011 174 604 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer