caa a22 4500 445881364 CHVBK 20180317145544.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s00604-011-0623-2 doi (NATIONALLICENCE)springer-10.1007/s00604-011-0623-2 Fluorescent probe for copper(II) ion based on a rhodamine spirolactame derivative, and its application to fluorescent imaging in living cells [Elektronische Daten] [Chunwei Yu, Jun Zhang, Jinhua Li, Ping Liu, Peihai Wei, Lingxin Chen] A fluorescent probe for Cu(II) ion is presented. It is based on the rhodamine fluorophore and exhibits high selectivity and sensitivity for Cu(II) ion in aqueous methanol (2:8, v/v) at pH 7.0. The response is based on a ring opening reaction and formation of a strongly fluorescent 1:1 complex. The response is reversible and linear in the range between 50nM and 900nM, with a detection limit of 7.0nM. The probe was successfully applied to fluorescent imaging of Cu(II) ions in HeLa cells. Figure A novel fluorescent probe 1 based on a rhodamine spirolactame derivative exhibits highly selective and sensitive recognition properties toward Cu(II) in aqueous methanol (2:8, v/v) at pH 7.0 with remarkable fluorescence enhancement and clear color change, and its high cell permeability grants its application to fluorescent imaging in living cells. Springer-Verlag, 2011 Copper(II) ions nationallicence Fluorescent probe nationallicence Fluorescence enhancement nationallicence Rhodamine derivatives nationallicence Fluorescence imaging nationallicence Living cells nationallicence Yu Chunwei Key Laboratory of Coastal Zone Environmental Processes, CAS; Shandong Provincial Key Laboratory of Coastal Zone Environmental Processes; Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, 264003, Yantai, China aut Zhang Jun School of Tropical and Laboratory Medicine, Hainan Medical University, 571101, Haikou, China aut Li Jinhua Key Laboratory of Coastal Zone Environmental Processes, CAS; Shandong Provincial Key Laboratory of Coastal Zone Environmental Processes; Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, 264003, Yantai, China aut Liu Ping Key Laboratory of Coastal Zone Environmental Processes, CAS; Shandong Provincial Key Laboratory of Coastal Zone Environmental Processes; Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, 264003, Yantai, China aut Wei Peihai Department of Chemistry, Qilu Normal University, 250013, Jinan, China aut Chen Lingxin Key Laboratory of Coastal Zone Environmental Processes, CAS; Shandong Provincial Key Laboratory of Coastal Zone Environmental Processes; Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, 264003, Yantai, China aut Microchimica Acta Springer Vienna 174/3-4(2011-09-01), 247-255 0026-3672 174:3-4<247 2011 174 604 https://doi.org/10.1007/s00604-011-0623-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00604-011-0623-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yu Chunwei Key Laboratory of Coastal Zone Environmental Processes, CAS; Shandong Provincial Key Laboratory of Coastal Zone Environmental Processes; Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, 264003, Yantai, China aut NATIONALLICENCE 700 1- Zhang Jun School of Tropical and Laboratory Medicine, Hainan Medical University, 571101, Haikou, China aut NATIONALLICENCE 700 1- Li Jinhua Key Laboratory of Coastal Zone Environmental Processes, CAS; Shandong Provincial Key Laboratory of Coastal Zone Environmental Processes; Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, 264003, Yantai, China aut NATIONALLICENCE 700 1- Liu Ping Key Laboratory of Coastal Zone Environmental Processes, CAS; Shandong Provincial Key Laboratory of Coastal Zone Environmental Processes; Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, 264003, Yantai, China aut NATIONALLICENCE 700 1- Wei Peihai Department of Chemistry, Qilu Normal University, 250013, Jinan, China aut NATIONALLICENCE 700 1- Chen Lingxin Key Laboratory of Coastal Zone Environmental Processes, CAS; Shandong Provincial Key Laboratory of Coastal Zone Environmental Processes; Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, 264003, Yantai, China aut NATIONALLICENCE 773 0- Microchimica Acta Springer Vienna 174/3-4(2011-09-01), 247-255 0026-3672 174:3-4<247 2011 174 604 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer