caa a22 4500 445881410 CHVBK 20180317145544.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s00604-011-0627-y doi (NATIONALLICENCE)springer-10.1007/s00604-011-0627-y New silica and polystyrene nanoparticles labeled with longwave absorbing and fluorescent chameleon dyes [Elektronische Daten] [Sayed Saleh, Reham Ali, Otto Wolfbeis] We are presenting new fluorescent nanoparticles (NPs) made from silica or polystyrene. Such NPs are potentially useful for purposes of cellular imaging and sensing. The NPs were surface-modified with amino groups, and longwave absorbing and emitting dyes were then conjugated, via their reactive chloro atoms, to the NPs. The reactions proceed at temperatures of around 65°C and in predominantly aqueous solution, and are accompanied by a color change from typically green to blue. By analogy to other labels giving this effect, we refer to such dyes as chameleon labels. All NPs were characterized in terms of size, by absorption and emission spectroscopy, thermogravimetry and zeta potentials. The chameleon effect also was used to detect the presence of minute quantities of amino groups on the surface of NPs, both by absorptiometry and, with particular sensitivity, by fluorescence. Figure Nanoparticles made from silica, polystyrene or lanthanide-doped NaYF4 are presented that have been labeled with a new class of longwave dyes that undergo a color change on reacting with surface amino groups (the so-called chameleon effect). This effect can also be used to detect the presence of amino groups on such particles. Springer-Verlag, 2011 Nanoparticles nationallicence Silica nationallicence Polystyrene nationallicence Fluorescence nationallicence Label nationallicence Saleh Sayed Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, 93040, Regensburg, Germany aut Ali Reham Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, 93040, Regensburg, Germany aut Wolfbeis Otto Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, 93040, Regensburg, Germany aut Microchimica Acta Springer Vienna 174/3-4(2011-09-01), 429-434 0026-3672 174:3-4<429 2011 174 604 https://doi.org/10.1007/s00604-011-0627-y text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00604-011-0627-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Saleh Sayed Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, 93040, Regensburg, Germany aut NATIONALLICENCE 700 1- Ali Reham Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, 93040, Regensburg, Germany aut NATIONALLICENCE 700 1- Wolfbeis Otto Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, 93040, Regensburg, Germany aut NATIONALLICENCE 773 0- Microchimica Acta Springer Vienna 174/3-4(2011-09-01), 429-434 0026-3672 174:3-4<429 2011 174 604 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer