caa a22 4500 445881437 CHVBK 20180317145544.0 cr unu---uuuuu 170323e20110701xx s 000 0 eng 10.1007/s00604-011-0606-3 doi (NATIONALLICENCE)springer-10.1007/s00604-011-0606-3 Luminescent probes for detection and imaging of hydrogen peroxide [Elektronische Daten] [Michael Schäferling, Dominik Grögel, Stephan Schreml] The relevance of hydrogen peroxide (H2O2) in biological processes has been underestimated for a long time. In recent years, various reports showed that H2O2 not only acts as a cytotoxic compound appearing in the course of oxidative stress, but also functions as an important signaling molecule. Fluorescent probes (or indicators) and nanoparticles that respond selectively to hydrogen peroxide can be applied for intracellular measurements or in vivo imaging, and are superior to electrochemical methods, e.g. in terms of spatial resolution. In contrast to previous reviews that concentrated on the adoption of different probes for certain applications, this survey highlights the basic principles of different probes in terms of their chemical design, structures and functionalities. Thus, the probes are classified according to the underlying reaction mechanism: oxidation, hydrolysis, photoinduced electron transfer, and lanthanide complexation. Other assays are based on fluorescent proteins and nanoparticles, and chemi- or bioluminescent reagents. We confine this review to probes that display a more or less distinct selectivity to hydrogen peroxide. Indicators responding to reactive oxygen species (ROS) in general, or to particular other ROS, are not covered. Finally, we briefly discuss future trends and perspectives of these luminescent reporters in biomedical research and imaging. Figure Luminescent probes and nanosensors are promising tools to study the role of H2O2 in cellular signal transduction processes, oxidative stress and wound healing. Advanced cell-penetrating probes paved the way to image intracellular concentrations of H2O2. This review highlights the development in the design of H2O2-sensitive probes over the past decade. Springer-Verlag, 2011 Hydrogen peroxide nationallicence Reactive oxygen species nationallicence Fluorescent probes nationallicence Fluorescence imaging nationallicence Cell signaling nationallicence Schäferling Michael Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, 93040, Regensburg, Germany aut Grögel Dominik Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, 93040, Regensburg, Germany aut Schreml Stephan Department of Dermatology, University Hospital Regensburg, Regensburg, Germany aut Microchimica Acta Springer Vienna 174/1-2(2011-07-01), 1-18 0026-3672 174:1-2<1 2011 174 604 https://doi.org/10.1007/s00604-011-0606-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00604-011-0606-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Schäferling Michael Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, 93040, Regensburg, Germany aut NATIONALLICENCE 700 1- Grögel Dominik Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, 93040, Regensburg, Germany aut NATIONALLICENCE 700 1- Schreml Stephan Department of Dermatology, University Hospital Regensburg, Regensburg, Germany aut NATIONALLICENCE 773 0- Microchimica Acta Springer Vienna 174/1-2(2011-07-01), 1-18 0026-3672 174:1-2<1 2011 174 604 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer