caa a22 4500 44588150X CHVBK 20180317145545.0 cr unu---uuuuu 170323e20110701xx s 000 0 eng 10.1007/s00604-011-0608-1 doi (NATIONALLICENCE)springer-10.1007/s00604-011-0608-1 Sensitive detection of hydrogen peroxide in foodstuff using an organic-inorganic hybrid multilayer-functionalized graphene biosensing platform [Elektronische Daten] [Yuling Cui, Bing Zhang, Bingqian Liu, Huafeng Chen, Guonan Chen, Dianping Tang] We report on a new electrochemical biosensing strategy for the sensitive detection of hydrogen peroxide (H2O2) in foodstuff samples. It is based on a gold electrode modified with layer of graphene patterned with a multilayer made from an organic-inorganic hybrid nanomaterial. Initially, a layer of thionine (Th) was assembled on the surface of the graphene nanosheets, and these were then cast on the surface of the electrode for the alternate assembly of gold nanoparticles and horseradish peroxidase. The large surface-to-volume ratio and high conductivity of the nanosheets provides a benign microenvironment for the construction of the biosensor. The use of such a multilayer not only shortens the electron transfer pathway of the active center of the enzyme due to the presence of gold nanoparticles, but also enhances the electrocatalytic efficiency of the biosensor toward the reduction of H2O2. The electrochemical characteristics of the biosensor were studied by cyclic voltammetry and chronoamperometry. The number of layers, the operating potential, and the pH of the supporting electrolyte were optimized. Linear response is obtained for the range from 0.5μM to 1.8mM of H2O2, the detection limit is 10 nM (at S/N = 3), and 95% of the steady-state current is reached within 2s. The method was applied to sense H2O2 in spiked sterilized milk and correlated excellently with the permanganate titration method. A new electrochemical biosensing strategy for sensitive detection of hydrogen peroxide in foodstuff was developed by using a gold electrode modified with a layer of graphene nanosheets patterned with a multilayer made from an organic-inorganic hybrid nanomaterial. Springer-Verlag, 2011 Electrochemical biosensor nationallicence Hydrogen peroxide nationallicence Graphene nationallicence Nanogold nationallicence Foodstuff nationallicence Cui Yuling Department of Chemistry, Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University, 350108, Fuzhou, People's Republic of China aut Zhang Bing Department of Chemistry, Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University, 350108, Fuzhou, People's Republic of China aut Liu Bingqian Department of Chemistry, Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University, 350108, Fuzhou, People's Republic of China aut Chen Huafeng Department of Chemistry, Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University, 350108, Fuzhou, People's Republic of China aut Chen Guonan Department of Chemistry, Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University, 350108, Fuzhou, People's Republic of China aut Tang Dianping Department of Chemistry, Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University, 350108, Fuzhou, People's Republic of China aut Microchimica Acta Springer Vienna 174/1-2(2011-07-01), 137-144 0026-3672 174:1-2<137 2011 174 604 https://doi.org/10.1007/s00604-011-0608-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00604-011-0608-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cui Yuling Department of Chemistry, Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University, 350108, Fuzhou, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Bing Department of Chemistry, Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University, 350108, Fuzhou, People's Republic of China aut NATIONALLICENCE 700 1- Liu Bingqian Department of Chemistry, Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University, 350108, Fuzhou, People's Republic of China aut NATIONALLICENCE 700 1- Chen Huafeng Department of Chemistry, Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University, 350108, Fuzhou, People's Republic of China aut NATIONALLICENCE 700 1- Chen Guonan Department of Chemistry, Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University, 350108, Fuzhou, People's Republic of China aut NATIONALLICENCE 700 1- Tang Dianping Department of Chemistry, Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University, 350108, Fuzhou, People's Republic of China aut NATIONALLICENCE 773 0- Microchimica Acta Springer Vienna 174/1-2(2011-07-01), 137-144 0026-3672 174:1-2<137 2011 174 604 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer