caa a22 4500 445881526 CHVBK 20180317145545.0 cr unu---uuuuu 170323e20110701xx s 000 0 eng 10.1007/s00604-011-0604-5 doi (NATIONALLICENCE)springer-10.1007/s00604-011-0604-5 Novel chemiluminescent assay for staphylococcal enterotoxin B [Elektronische Daten] [Pan Xue, Yongming Li, Zhujun Zhang, Aihua Fu, Fei Liu, Xiaoming Zhang, Yuanjie Sun, Lili Chen, Boquan Jin, Kun Yang] An enzyme-linked immunosorbent assay, a horseradish peroxidase-catalyzed fluorogenic reaction, and chemiluminescence (CL) analysis have been combined to develop a sandwich ELISA for Staphylococcal enterotoxin B (SEB) using monoclonal antibodies for different epitopes of SEB. The enzyme catalyzed reaction of 3-(4-hydroxyphenyl propionate) with the urea complex of hydrogen peroxide produced a fluorescent dimer which was detected by chemiluminescence analysis. The CL response to SEB is linear in the range from 6.0 to 564pgmL−1 (r = 0.9993), and the detection limit is 3.3pgmL−1 (S/N = 3). Intra- and interassay coefficients of variation are <7.0% at three concentrations (24, 96 and 384pgmL−1). The method was applied to the analysis of SEB in serum, lake water and milk samples. The results compared well with those obtained by conventional ELISAs. Figure Procedures of the proposed method. A sandwich ELISA for Staphylococcal enterotoxin B (SEB) using a pair of monoclonal antibodies that recognizes different epitopes of SEB. After the ELISA procedure, PHPPA is reacted with Hydrogen peroxide-urea, with catalysis by HRP-conjugated anti-SEB, to produce PHPPA fluorescent Dimer, which is detected by TCPO chemiluminescence. Springer-Verlag, 2011 Staphylococcal enterotoxin B (SEB) nationallicence Enzyme-linked immunosorbent assays nationallicence Horseradish peroxidase-catalyzed fluorescent reaction nationallicence Chemiluminescence analysis nationallicence Xue Pan Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Materials Science, Shaanxi Normal University, 710062, Xi'an, China aut Li Yongming Department of Immunology, The Fourth Military Medical University, 710032, Xi'an, China aut Zhang Zhujun Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Materials Science, Shaanxi Normal University, 710062, Xi'an, China aut Fu Aihua Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Materials Science, Shaanxi Normal University, 710062, Xi'an, China aut Liu Fei Department of Immunology, The Fourth Military Medical University, 710032, Xi'an, China aut Zhang Xiaoming Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Materials Science, Shaanxi Normal University, 710062, Xi'an, China aut Sun Yuanjie Department of Immunology, The Fourth Military Medical University, 710032, Xi'an, China aut Chen Lili Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Materials Science, Shaanxi Normal University, 710062, Xi'an, China aut Jin Boquan Department of Immunology, The Fourth Military Medical University, 710032, Xi'an, China aut Yang Kun Department of Immunology, The Fourth Military Medical University, 710032, Xi'an, China aut Microchimica Acta Springer Vienna 174/1-2(2011-07-01), 167-174 0026-3672 174:1-2<167 2011 174 604 https://doi.org/10.1007/s00604-011-0604-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00604-011-0604-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Xue Pan Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Materials Science, Shaanxi Normal University, 710062, Xi'an, China aut NATIONALLICENCE 700 1- Li Yongming Department of Immunology, The Fourth Military Medical University, 710032, Xi'an, China aut NATIONALLICENCE 700 1- Zhang Zhujun Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Materials Science, Shaanxi Normal University, 710062, Xi'an, China aut NATIONALLICENCE 700 1- Fu Aihua Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Materials Science, Shaanxi Normal University, 710062, Xi'an, China aut NATIONALLICENCE 700 1- Liu Fei Department of Immunology, The Fourth Military Medical University, 710032, Xi'an, China aut NATIONALLICENCE 700 1- Zhang Xiaoming Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Materials Science, Shaanxi Normal University, 710062, Xi'an, China aut NATIONALLICENCE 700 1- Sun Yuanjie Department of Immunology, The Fourth Military Medical University, 710032, Xi'an, China aut NATIONALLICENCE 700 1- Chen Lili Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Materials Science, Shaanxi Normal University, 710062, Xi'an, China aut NATIONALLICENCE 700 1- Jin Boquan Department of Immunology, The Fourth Military Medical University, 710032, Xi'an, China aut NATIONALLICENCE 700 1- Yang Kun Department of Immunology, The Fourth Military Medical University, 710032, Xi'an, China aut NATIONALLICENCE 773 0- Microchimica Acta Springer Vienna 174/1-2(2011-07-01), 167-174 0026-3672 174:1-2<167 2011 174 604 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer