caa a22 4500 445881569 CHVBK 20180317145545.0 cr unu---uuuuu 170323e20110701xx s 000 0 eng 10.1007/s00604-011-0585-4 doi (NATIONALLICENCE)springer-10.1007/s00604-011-0585-4 Biocompatible and label-free amperometric immunosensor for hepatitis B surface antigen using a sensing film composed of poly(allylamine)-branched ferrocene and gold nanoparticles [Elektronische Daten] [Jian-Ding Qiu, He Huang, Ru-Ping Liang] An amperometric immunosensor has been developed for sensitive determination of hepatitis B surface antigen as a model protein. A glassy carbon electrode was modified with an assembly of positively charged poly(allylamine)-branched ferrocene (PAA-Fc) and negatively charged gold nanoparticles (Au NPs). The formation of PAA-Fc effectively avoids the leakage of Fc, retains its electrochemical activity, and enhances the conductivity of the composite. The adsorption of Au NPs onto the PAA-Fc matrix provides sites for the immobilization of the antigen and a favorable micro-environment to maintain its activity. The morphologies and electrochemistry of the sensing film were investigated via scanning electron microscopy, electrochemical impedance spectroscopy, and cyclic voltammetry. Factors influencing the performance of the immunosensor were studied in detail. The concentration of the antigen can be quantitated (by measuring the decrease of the amperometric response resulting from the specific binding between antigen and antibody) in the range between 0.1 and 150ngmL-1, with a detection limit of 40pgmL-1 (S/N = 3). The method is economical, efficient, and potentially attractive for clinical immunoassays. Figure A novel and sensitive amperometric immunosensor based on the assembly of biocompatible positively charged poly(allylamine)-branched ferrocene and negatively charged Au nanoparticles onto a glassy carbon electrode has been developed for sensitive determination of hepatitis B surface antigen as a model protein. Springer-Verlag, 2011 Amperometric immunosensor nationallicence Poly(allylamine) nationallicence Ferrocene nationallicence Gold nanoparticles nationallicence Hepatitis B surface antigen nationallicence Qiu Jian-Ding Department of Chemistry and Institute for Advanced Study, Nanchang University, 330031, Nanchang, People's Republic of China aut Huang He Department of Chemistry and Institute for Advanced Study, Nanchang University, 330031, Nanchang, People's Republic of China aut Liang Ru-Ping Department of Chemistry and Institute for Advanced Study, Nanchang University, 330031, Nanchang, People's Republic of China aut Microchimica Acta Springer Vienna 174/1-2(2011-07-01), 97-105 0026-3672 174:1-2<97 2011 174 604 https://doi.org/10.1007/s00604-011-0585-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00604-011-0585-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Qiu Jian-Ding Department of Chemistry and Institute for Advanced Study, Nanchang University, 330031, Nanchang, People's Republic of China aut NATIONALLICENCE 700 1- Huang He Department of Chemistry and Institute for Advanced Study, Nanchang University, 330031, Nanchang, People's Republic of China aut NATIONALLICENCE 700 1- Liang Ru-Ping Department of Chemistry and Institute for Advanced Study, Nanchang University, 330031, Nanchang, People's Republic of China aut NATIONALLICENCE 773 0- Microchimica Acta Springer Vienna 174/1-2(2011-07-01), 97-105 0026-3672 174:1-2<97 2011 174 604 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer