caa a22 4500 445881577 CHVBK 20180317145545.0 cr unu---uuuuu 170323e20110701xx s 000 0 eng 10.1007/s00604-011-0592-5 doi (NATIONALLICENCE)springer-10.1007/s00604-011-0592-5 Simple amperometric detector for microchip capillary electrophoresis, and its application to the analysis of dopamine and catechol [Elektronische Daten] [Xinchun Li, Jianbin Pan, Fan Yang, Jun Feng, Jinyuan Mo, Zuanguang Chen] We report on a simple amperometric detector for use in microchip capillary electrophoresis. A disposable syringe serves as the electrode holder that is fixed at the outlet of the separation channel. A carbon paste electrode is used to detect dopamine (DA) and catechol (CA) after electrophoretic separation. The two model analytes were well separated within 60 s. The response is linear in the concentration range from 4 to 500μM, and the detection limit is 1.2μM for DA (S/N = 3:1). The relative standard deviations of the inter-run and inter-electrode peak currents, respectively, are 2.8% and 5.7% for DA, and 3.9% and 6.5% for CA. Favorable column efficiency (expressed by the theoretical plate number which is 5.3 × 104 m-1 for DA) is achieved. The method was successfully applied to the separation and detection of 3-aminophenol (3-AP) in an injection powder containing sodium 4-aminosalicylate. The detection limit of 3-AP is as low as 1.7μM, which meets the demand of the impurity test. The facile assembly allows convenient replacement of working electrodes and improves the longevity of the microanalytical system. Figure Voltammetric behaviors of 3-AP and 4-AS at carbon paste electrode (CPE), and microchip electrophoresis-amperometry with CPE for the two analytes. Springer-Verlag, 2011 Amperometric detection nationallicence Microchip capillary electrophoresis nationallicence Carbon paste electrode nationallicence 3-aminophenol nationallicence Drug impurity test nationallicence Li Xinchun School of Pharmaceutical Sciences, Sun Yat-sen University, 510006, Guangzhou, People's Republic of China aut Pan Jianbin School of Pharmaceutical Sciences, Sun Yat-sen University, 510006, Guangzhou, People's Republic of China aut Yang Fan School of Pharmaceutical Sciences, Sun Yat-sen University, 510006, Guangzhou, People's Republic of China aut Feng Jun School of Pharmaceutical Sciences, Sun Yat-sen University, 510006, Guangzhou, People's Republic of China aut Mo Jinyuan School of Chemistry and Chemical Engineer, Sun Yat-sen University, 510275, Guangzhou, People's Republic of China aut Chen Zuanguang School of Pharmaceutical Sciences, Sun Yat-sen University, 510006, Guangzhou, People's Republic of China aut Microchimica Acta Springer Vienna 174/1-2(2011-07-01), 123-130 0026-3672 174:1-2<123 2011 174 604 https://doi.org/10.1007/s00604-011-0592-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00604-011-0592-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li Xinchun School of Pharmaceutical Sciences, Sun Yat-sen University, 510006, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Pan Jianbin School of Pharmaceutical Sciences, Sun Yat-sen University, 510006, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Yang Fan School of Pharmaceutical Sciences, Sun Yat-sen University, 510006, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Feng Jun School of Pharmaceutical Sciences, Sun Yat-sen University, 510006, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Mo Jinyuan School of Chemistry and Chemical Engineer, Sun Yat-sen University, 510275, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Chen Zuanguang School of Pharmaceutical Sciences, Sun Yat-sen University, 510006, Guangzhou, People's Republic of China aut NATIONALLICENCE 773 0- Microchimica Acta Springer Vienna 174/1-2(2011-07-01), 123-130 0026-3672 174:1-2<123 2011 174 604 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer