caa a22 4500 445881607 CHVBK 20180317145545.0 cr unu---uuuuu 170323e20110701xx s 000 0 eng 10.1007/s00604-011-0613-4 doi (NATIONALLICENCE)springer-10.1007/s00604-011-0613-4 Preparation and characterization of molecularly imprinted microspheres for selective extraction of trace melamine from milk samples [Elektronische Daten] [Bin Wang, Yuzhen Wang, Hong Yang, Junqing Wang, Anping Deng] We describe molecularly imprinted microspheres (MIMs) for the selective extraction of melamine from milk. The MIMs were made from melamine as the template molecule, methacrylic acid as the functional monomer, and ethylene glycol dimethacrylate as the linking agent. The MIMs were synthesized by suspension polymerization and characterized by rebinding experiments. They displayed high adsorption capacity, fast rebinding kinetics, and highly specific rebinding of melamine. The imprinting factor is 4.1. Scatchard analysis revealed a one-type rebinding behavior, the dissociation constant and maximum rebinding capacity being 37.59gL−1 and 30.85μmolg−1, respectively. The MIMs exhibited a 25% cross-reactivity towards atrazine, but less than 3.0% towards prometryn, clenbuterol and metronidazole. In addition, a MIM-based solid phase extraction (MISPE) column for melamine was prepared by packing MIMs into a common SPE cartridge. The MISPE extraction gave recoveries of 89.8 to 100.6% of melamine, with relative standard deviations of 5.9 to 7.5%. There was no significant loss of rebinding capacity after more than 60 repeated uses, thus demonstrating the high stability of the MISPE column. The MSPE column also was applied to the extraction of melamine from spiked liquid and powdered milk with satisfying accuracy and precision. Figure The melamine molecularly imprinted microsphere (MIMs) prepared by suspension polymerization displayed high adsorption capacity quick rebinding kinetic and highly specific rebinding. The MIMs-based solid phase extraction (MISPE) column was prepared by packing MIMs into a common SPE cartridge. The recoveries of MISPE for extracting melamine from milk samples were 76.26-90.95%. Springer-Verlag, 2011 Molecular imprinted microspheres (MIMs) nationallicence Melamine nationallicence Extraction nationallicence Milk sample nationallicence Wang Bin College of Chemistry, Sichuan University, 610064, Chengdu, China aut Wang Yuzhen College of Chemistry, Sichuan University, 610064, Chengdu, China aut Yang Hong College of Pharmacy, Soochow University, 215123, Suzhou, China aut Wang Junqing College of Chemistry, Sichuan University, 610064, Chengdu, China aut Deng Anping College of Chemistry, Sichuan University, 610064, Chengdu, China aut Microchimica Acta Springer Vienna 174/1-2(2011-07-01), 191-199 0026-3672 174:1-2<191 2011 174 604 https://doi.org/10.1007/s00604-011-0613-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00604-011-0613-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Bin College of Chemistry, Sichuan University, 610064, Chengdu, China aut NATIONALLICENCE 700 1- Wang Yuzhen College of Chemistry, Sichuan University, 610064, Chengdu, China aut NATIONALLICENCE 700 1- Yang Hong College of Pharmacy, Soochow University, 215123, Suzhou, China aut NATIONALLICENCE 700 1- Wang Junqing College of Chemistry, Sichuan University, 610064, Chengdu, China aut NATIONALLICENCE 700 1- Deng Anping College of Chemistry, Sichuan University, 610064, Chengdu, China aut NATIONALLICENCE 773 0- Microchimica Acta Springer Vienna 174/1-2(2011-07-01), 191-199 0026-3672 174:1-2<191 2011 174 604 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer