caa a22 4500 445882352 CHVBK 20180317145547.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s10157-010-0370-4 doi (NATIONALLICENCE)springer-10.1007/s10157-010-0370-4 CD28 superagonist-induced regulatory T cell expansion ameliorates mesangioproliferative glomerulonephritis in rats [Elektronische Daten] [Kenro Miyasato, Yoshitsugu Takabatake, Junya Kaimori, Tomonori Kimura, Harumi Kitamura, Hiroshi Kawachi, Xiao-Kang Li, Thomas Hünig, Shiro Takahara, Hiromi Rakugi, Yoshitaka Isaka] Background: Naturally occurring regulatory T cells (Treg) are essential for the prevention of autoimmunity and overshooting immune responses to pathogens; however, the involvement of Treg in mesangioproliferative glomerulonephritis, a major cause of chronic kidney disease, remains unclear. Superagonistic CD28-specific monoclonal antibodies (CD28SA) are highly effective activators of Treg in rats. Method: To confirm our hypothesis that CD28SA reduces the severity of experimental glomerulonephritis, anti-Thy1 nephritis model rats were treated with CD28SA or saline. Results: CD28SA significantly suppressed the increase in proteinuria and serum creatinine levels. CD28SA-treated nephritic rats exhibited an increase in the infiltration of Treg in the glomeruli accompanied by infiltration of CD163-positive macrophages ("alternatively activated” macrophages). In addition, CD28SA significantly induced interleukin-10 mRNA expression in glomeruli, thereby ameliorating mesangial cell proliferation and extracellular matrix expansion. Conclusion: We established a new therapeutic approach to suppressing progressive glomerulonephritis. The therapeutic value of this approach warrants further attention and preclinical studies. Japanese Society of Nephrology, 2010 CD28 nationallicence Regulatory T cell nationallicence FoxP3 nationallicence "Alternatively activated” macrophage nationallicence Miyasato Kenro Department of Geriatric Medicine and Nephrology, Osaka University Graduate School of Medicine, 2-2 Yamada-oka, 565-0871, Suita, Osaka, Japan aut Takabatake Yoshitsugu Department of Geriatric Medicine and Nephrology, Osaka University Graduate School of Medicine, 2-2 Yamada-oka, 565-0871, Suita, Osaka, Japan aut Kaimori Junya Department of Advanced Technology for Transplantation, Osaka University Graduate School of Medicine, Suita, Osaka, Japan aut Kimura Tomonori Department of Geriatric Medicine and Nephrology, Osaka University Graduate School of Medicine, 2-2 Yamada-oka, 565-0871, Suita, Osaka, Japan aut Kitamura Harumi Department of Geriatric Medicine and Nephrology, Osaka University Graduate School of Medicine, 2-2 Yamada-oka, 565-0871, Suita, Osaka, Japan aut Kawachi Hiroshi Department of Cell Biology, Institute of Nephrology, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan aut Li Xiao-Kang Laboratory of Transplantation Immunology, National Research Institute for Child Health and Development, Tokyo, Japan aut Hünig Thomas Institut für Virologie and Immunobiologie, Universtät Würzburg, Würzburg, Germany aut Takahara Shiro Department of Advanced Technology for Transplantation, Osaka University Graduate School of Medicine, Suita, Osaka, Japan aut Rakugi Hiromi Department of Geriatric Medicine and Nephrology, Osaka University Graduate School of Medicine, 2-2 Yamada-oka, 565-0871, Suita, Osaka, Japan aut Isaka Yoshitaka Department of Geriatric Medicine and Nephrology, Osaka University Graduate School of Medicine, 2-2 Yamada-oka, 565-0871, Suita, Osaka, Japan aut Clinical and Experimental Nephrology Springer Japan 15/1(2011-02-01), 50-57 1342-1751 15:1<50 2011 15 10157 https://doi.org/10.1007/s10157-010-0370-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10157-010-0370-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Miyasato Kenro Department of Geriatric Medicine and Nephrology, Osaka University Graduate School of Medicine, 2-2 Yamada-oka, 565-0871, Suita, Osaka, Japan aut NATIONALLICENCE 700 1- Takabatake Yoshitsugu Department of Geriatric Medicine and Nephrology, Osaka University Graduate School of Medicine, 2-2 Yamada-oka, 565-0871, Suita, Osaka, Japan aut NATIONALLICENCE 700 1- Kaimori Junya Department of Advanced Technology for Transplantation, Osaka University Graduate School of Medicine, Suita, Osaka, Japan aut NATIONALLICENCE 700 1- Kimura Tomonori Department of Geriatric Medicine and Nephrology, Osaka University Graduate School of Medicine, 2-2 Yamada-oka, 565-0871, Suita, Osaka, Japan aut NATIONALLICENCE 700 1- Kitamura Harumi Department of Geriatric Medicine and Nephrology, Osaka University Graduate School of Medicine, 2-2 Yamada-oka, 565-0871, Suita, Osaka, Japan aut NATIONALLICENCE 700 1- Kawachi Hiroshi Department of Cell Biology, Institute of Nephrology, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan aut NATIONALLICENCE 700 1- Li Xiao-Kang Laboratory of Transplantation Immunology, National Research Institute for Child Health and Development, Tokyo, Japan aut NATIONALLICENCE 700 1- Hünig Thomas Institut für Virologie and Immunobiologie, Universtät Würzburg, Würzburg, Germany aut NATIONALLICENCE 700 1- Takahara Shiro Department of Advanced Technology for Transplantation, Osaka University Graduate School of Medicine, Suita, Osaka, Japan aut NATIONALLICENCE 700 1- Rakugi Hiromi Department of Geriatric Medicine and Nephrology, Osaka University Graduate School of Medicine, 2-2 Yamada-oka, 565-0871, Suita, Osaka, Japan aut NATIONALLICENCE 700 1- Isaka Yoshitaka Department of Geriatric Medicine and Nephrology, Osaka University Graduate School of Medicine, 2-2 Yamada-oka, 565-0871, Suita, Osaka, Japan aut NATIONALLICENCE 773 0- Clinical and Experimental Nephrology Springer Japan 15/1(2011-02-01), 50-57 1342-1751 15:1<50 2011 15 10157 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer