caa a22 4500 445882972 CHVBK 20180317145550.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s10157-011-0414-4 doi (NATIONALLICENCE)springer-10.1007/s10157-011-0414-4 Upregulation of calbindin D28k in the late distal tubules in the potassium-loaded adrenalectomized mouse kidney [Elektronische Daten] [Mizuka Kobayashi, Yukiko Yasuoka, Yuichi Sato, Ming Zhou, Hiroshi Abe, Katsumasa Kawahara, Hirotsugu Okamoto] Background: The calcium (Ca)-activated potassium (K) channel is an alternative K-secretory pathway in the apical membranes of the distal nephrons of adrenalectomized (ADX) animals. As a potential approach for estimating intracellular Ca2+ increase, we investigated normal and ADX mice to determine whether dietary K intake would stimulate the expression of the calbindin D28k protein, a cytosolic Ca2+-binding protein, along the distal nephron consisting of the early and late portions of the distal convoluted tubule (DCT1 and DCT2, respectively), the CNT, and CCD. Methods: ADX mice received a control diet plus either 0.3% NaCl solution (C) or a 0.3% NaCl plus 3% KCl solution (HK) for 7days before the experiment. Results: The mean plasma K concentration and pH were significantly (P<0.001) higher (7.9±0.3mEq/l) and lower (7.28±0.02) in the K-loaded ADX mice than in the control ADX mice. The mean urinary K excretion (mEq/day) and urine flow (ml/day) increased significantly (P<0.0001) from 0.47±0.07 (C) to 4.80±0.57 (HK) and from 1.1±0.2 (C) to 8.8±1.0 (HK). Urinary Ca excretion significantly (P<0.005 and P<0.05, respectively) increased in K-loaded normal and ADX mice compared with control normal and ADX mice. Immunofluorescence studies revealed that the relative staining of calbindin was 167.0±15.4%, 291.3±13.8%, and 206.3±11.3% for DCT1, DCT2/CNT, and CCD of normal control mice, respectively. These values increased significantly (P<0.0001) only in DCT2/CNT (574.8±42%) of the K-loaded ADX mice. Conclusion: Upregulation of calbindin in the late distal tubule suggests that Ca2+-dependent K transport may function as an alternative mechanism for urinary K excretion in ADX mice. Japanese Society of Nephrology, 2011 Hyperkalemia nationallicence Hyperchloremic metabolic acidosis nationallicence Hypercalciuria nationallicence Ca2+-dependent K excretion in distal tubule nationallicence Kobayashi Mizuka Department of Anesthesiology, Kitasato University Graduate School of Medical Sciences, 1-15-1 Kitasato, Minami-ku, 252-0374, Sagamihara, Japan aut Yasuoka Yukiko Department of Cellular and Molecular Physiology, Kitasato University Graduate School of Medical Sciences, 1-15-1 Kitasato, Minami-ku, 252-0374, Sagamihara, Japan aut Sato Yuichi Department of Applied Tumor Pathology, Kitasato University Graduate School of Medical Sciences, 1-15-1 Kitasato, Minami-ku, Sagamihara, Japan aut Zhou Ming Department of Anatomy, Akita University Graduate School of Medicine and Faculty of Medicine, 1-1-1 Hondo, Akita, Japan aut Abe Hiroshi Department of Anatomy, Akita University Graduate School of Medicine and Faculty of Medicine, 1-1-1 Hondo, Akita, Japan aut Kawahara Katsumasa Department of Cellular and Molecular Physiology, Kitasato University Graduate School of Medical Sciences, 1-15-1 Kitasato, Minami-ku, 252-0374, Sagamihara, Japan aut Okamoto Hirotsugu Department of Anesthesiology, Kitasato University Graduate School of Medical Sciences, 1-15-1 Kitasato, Minami-ku, 252-0374, Sagamihara, Japan aut Clinical and Experimental Nephrology Springer Japan 15/3(2011-06-01), 355-362 1342-1751 15:3<355 2011 15 10157 https://doi.org/10.1007/s10157-011-0414-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10157-011-0414-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kobayashi Mizuka Department of Anesthesiology, Kitasato University Graduate School of Medical Sciences, 1-15-1 Kitasato, Minami-ku, 252-0374, Sagamihara, Japan aut NATIONALLICENCE 700 1- Yasuoka Yukiko Department of Cellular and Molecular Physiology, Kitasato University Graduate School of Medical Sciences, 1-15-1 Kitasato, Minami-ku, 252-0374, Sagamihara, Japan aut NATIONALLICENCE 700 1- Sato Yuichi Department of Applied Tumor Pathology, Kitasato University Graduate School of Medical Sciences, 1-15-1 Kitasato, Minami-ku, Sagamihara, Japan aut NATIONALLICENCE 700 1- Zhou Ming Department of Anatomy, Akita University Graduate School of Medicine and Faculty of Medicine, 1-1-1 Hondo, Akita, Japan aut NATIONALLICENCE 700 1- Abe Hiroshi Department of Anatomy, Akita University Graduate School of Medicine and Faculty of Medicine, 1-1-1 Hondo, Akita, Japan aut NATIONALLICENCE 700 1- Kawahara Katsumasa Department of Cellular and Molecular Physiology, Kitasato University Graduate School of Medical Sciences, 1-15-1 Kitasato, Minami-ku, 252-0374, Sagamihara, Japan aut NATIONALLICENCE 700 1- Okamoto Hirotsugu Department of Anesthesiology, Kitasato University Graduate School of Medical Sciences, 1-15-1 Kitasato, Minami-ku, 252-0374, Sagamihara, Japan aut NATIONALLICENCE 773 0- Clinical and Experimental Nephrology Springer Japan 15/3(2011-06-01), 355-362 1342-1751 15:3<355 2011 15 10157 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer