caa a22 4500 445882980 CHVBK 20180317145550.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s10157-011-0408-2 doi (NATIONALLICENCE)springer-10.1007/s10157-011-0408-2 Growth factor Midkine is involved in the pathogenesis of renal injury induced by protein overload containing endotoxin [Elektronische Daten] [Kiyonari Kato, Tomoki Kosugi, Waichi Sato, Hanayo Arata-Kawai, Takenori Ozaki, Naotake Tsuboi, Isao Ito, Hideo Tawada, Yukio Yuzawa, Seiichi Matsuo, Kenji Kadomatsu, Shoichi Maruyama] Background: Growth factor Midkine (MK), which expresses on endothelial cells and renal proximal tubules, has been implicated in inflammation-related kidney diseases such as ischemic reperfusion-induced tubulointerstitial injury and diabetic nephropathy. The biological actions of MK are elicited through its chemotactic activity and chemokine-driven inflammatory pathway. Post-infectious glomerulonephritis is caused by the deposition of immune complexes into glomeruli by infiltrating a number of inflammatory cells. Therefore, we investigated whether MK might be involved in the pathogenesis of acute glomerulonephritis. Methods: We induced endocapillary proliferative glomerulonephritis in 129/SV mice using intraperitoneal injections of a large amount of protein. Results: In contrast to mice deficient in MK (Mdk −/−), Mdk +/+ mice induced by protein overload demonstrated more diffuse cellular proliferation in the mesangial areas and capillary lumens, eventually leading to glomerular damage and tubulointerstitial injury. This pathological observation could be attributable to neutrophil infiltration through the chemotaxis and stimulation of the MK-macrophage inflammatory protein (MIP)-2 pathway, but appeared to be due to the MK-related immunoglobulin (Ig)G deposition and C3 activation. These findings are often seen in infectious-related glomerular injury. Furthermore, the profile of MK expression was strongly consistent with that of glomerular damage and tubulointersititial injury. Conclusion: This study might provide a new insight into understanding the deleterious role of MK in endocapillary proliferative glomerulonephritis induced by protein overload. Japanese Society of Nephrology, 2011 Midkine nationallicence Protein-overload mouse model nationallicence Endocapillary proliferative glomerulonephritis nationallicence Kato Kiyonari Division of Nephrology, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, 466-8550, Nagoya, Aichi, Japan aut Kosugi Tomoki Division of Nephrology, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, 466-8550, Nagoya, Aichi, Japan aut Sato Waichi Division of Nephrology, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, 466-8550, Nagoya, Aichi, Japan aut Arata-Kawai Hanayo Division of Nephrology, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, 466-8550, Nagoya, Aichi, Japan aut Ozaki Takenori Division of Nephrology, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, 466-8550, Nagoya, Aichi, Japan aut Tsuboi Naotake Division of Nephrology, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, 466-8550, Nagoya, Aichi, Japan aut Ito Isao Division of Nephrology, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, 466-8550, Nagoya, Aichi, Japan aut Tawada Hideo Tawada Clinic, 2-15-23 Kaminagoya Nishi-ku, 451-0025, Nagoya, Aichi, Japan aut Yuzawa Yukio Division of Nephrology, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, 466-8550, Nagoya, Aichi, Japan aut Matsuo Seiichi Division of Nephrology, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, 466-8550, Nagoya, Aichi, Japan aut Kadomatsu Kenji Division of Biochemistry, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, 466-8550, Nagoya, Aichi, Japan aut Maruyama Shoichi Division of Nephrology, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, 466-8550, Nagoya, Aichi, Japan aut Clinical and Experimental Nephrology Springer Japan 15/3(2011-06-01), 346-354 1342-1751 15:3<346 2011 15 10157 https://doi.org/10.1007/s10157-011-0408-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10157-011-0408-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kato Kiyonari Division of Nephrology, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, 466-8550, Nagoya, Aichi, Japan aut NATIONALLICENCE 700 1- Kosugi Tomoki Division of Nephrology, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, 466-8550, Nagoya, Aichi, Japan aut NATIONALLICENCE 700 1- Sato Waichi Division of Nephrology, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, 466-8550, Nagoya, Aichi, Japan aut NATIONALLICENCE 700 1- Arata-Kawai Hanayo Division of Nephrology, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, 466-8550, Nagoya, Aichi, Japan aut NATIONALLICENCE 700 1- Ozaki Takenori Division of Nephrology, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, 466-8550, Nagoya, Aichi, Japan aut NATIONALLICENCE 700 1- Tsuboi Naotake Division of Nephrology, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, 466-8550, Nagoya, Aichi, Japan aut NATIONALLICENCE 700 1- Ito Isao Division of Nephrology, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, 466-8550, Nagoya, Aichi, Japan aut NATIONALLICENCE 700 1- Tawada Hideo Tawada Clinic, 2-15-23 Kaminagoya Nishi-ku, 451-0025, Nagoya, Aichi, Japan aut NATIONALLICENCE 700 1- Yuzawa Yukio Division of Nephrology, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, 466-8550, Nagoya, Aichi, Japan aut NATIONALLICENCE 700 1- Matsuo Seiichi Division of Nephrology, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, 466-8550, Nagoya, Aichi, Japan aut NATIONALLICENCE 700 1- Kadomatsu Kenji Division of Biochemistry, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, 466-8550, Nagoya, Aichi, Japan aut NATIONALLICENCE 700 1- Maruyama Shoichi Division of Nephrology, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, 466-8550, Nagoya, Aichi, Japan aut NATIONALLICENCE 773 0- Clinical and Experimental Nephrology Springer Japan 15/3(2011-06-01), 346-354 1342-1751 15:3<346 2011 15 10157 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer