caa a22 4500 445883359 CHVBK 20180317145551.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s10157-011-0424-2 doi (NATIONALLICENCE)springer-10.1007/s10157-011-0424-2 Altered monocyte-derived dendritic cell function in patients on hemodialysis: a culprit for underlying impaired immune responses [Elektronische Daten] [Hye Choi, Young Woo, Myung Kim, Sang-Kyung Jo, Won Cho, Hyoung Kim] Background: Patients with end-stage renal disease (ESRD) are known to have impaired immune function. Dendritic cells (DCs) are the major antigen-presenting cells that initiate primary immune responses, linking innate and adaptive immunity. Although suboptimal immune responses to vaccination, as frequently observed in ESRD patients, might suggest the presence of impaired DC function, the precise nature of altered DC function is not fully understood. Methods: In the current study, we compared the maturation status, viability, and function of monocyte-derived DCs (moDCs) of patients on hemodialysis (HD) with healthy controls. Results: Surface expression of major histocompatibility complex class II, CD83, and CD86, and chemokine receptor CCR7 in moDCs was not different between HD patients and healthy controls. No significant difference was detected in the viability of moDCs determined by expression of annexin V and propidium iodide between two groups. However, moDCs from HD patients produced significantly higher amounts of IL-6 when stimulated by cytokine cocktails compared to healthy controls. In addition, mature moDCs from HD patients showed significantly enhanced allogeneic T-cell proliferation compared to healthy controls. Conclusions: Our data demonstrate aberrant DC function in HD patients and suggest that this might contribute to impaired immune responses. Japanese Society of Nephrology, 2011 End-stage renal disease nationallicence Immune responses nationallicence Function of dendritic cells nationallicence Choi Hye Division of Nephrology, Department of Internal Medicine, Korea University Medical College, Seoul, Korea aut Woo Young Division of Nephrology, Department of Internal Medicine, Korea University Medical College, Seoul, Korea aut Kim Myung Division of Nephrology, Department of Internal Medicine, Korea University Medical College, Seoul, Korea aut Jo Sang-Kyung Division of Nephrology, Department of Internal Medicine, Korea University Medical College, Seoul, Korea aut Cho Won Division of Nephrology, Department of Internal Medicine, Korea University Medical College, Seoul, Korea aut Kim Hyoung Division of Nephrology, Department of Internal Medicine, Korea University Medical College, Seoul, Korea aut Clinical and Experimental Nephrology Springer Japan 15/4(2011-08-01), 546-553 1342-1751 15:4<546 2011 15 10157 https://doi.org/10.1007/s10157-011-0424-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10157-011-0424-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Choi Hye Division of Nephrology, Department of Internal Medicine, Korea University Medical College, Seoul, Korea aut NATIONALLICENCE 700 1- Woo Young Division of Nephrology, Department of Internal Medicine, Korea University Medical College, Seoul, Korea aut NATIONALLICENCE 700 1- Kim Myung Division of Nephrology, Department of Internal Medicine, Korea University Medical College, Seoul, Korea aut NATIONALLICENCE 700 1- Jo Sang-Kyung Division of Nephrology, Department of Internal Medicine, Korea University Medical College, Seoul, Korea aut NATIONALLICENCE 700 1- Cho Won Division of Nephrology, Department of Internal Medicine, Korea University Medical College, Seoul, Korea aut NATIONALLICENCE 700 1- Kim Hyoung Division of Nephrology, Department of Internal Medicine, Korea University Medical College, Seoul, Korea aut NATIONALLICENCE 773 0- Clinical and Experimental Nephrology Springer Japan 15/4(2011-08-01), 546-553 1342-1751 15:4<546 2011 15 10157 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer