caa a22 4500 445884797 CHVBK 20180317145555.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s00011-010-0232-2 doi (NATIONALLICENCE)springer-10.1007/s00011-010-0232-2 Toll-like receptor 4 signaling in dysfunction of cardiac microvascular endothelial cells under hypoxia/reoxygenation [Elektronische Daten] [Zheng Zhang, Weijie Li, Dongdong Sun, Li Zhao, Rongqing Zhang, Yabin Wang, Xuan Zhou, Haichang Wang, Feng Cao] Objective: This study was designed to detect the role of Toll-like receptor 4 (TLR4) signaling in the dysfunction of cardiac microvascular endothelial cells (CMECs) after hypoxia/reoxygenation (H/R). Methods: The cell viability of CMECs was measured by MTT assay. The migration of CMECs was detected by cell scratch wound assay. The expressions of TLR4, nuclear factor-kappa B (NF-κB) and eNOS were analyzed by Western blot. Secretions of nitric oxide (NO) and tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) were determined by NO detection kit and ELISA. Results: Lipopolysaccharide (LPS) incubation increased the expressions of TLR4, NF-κB, IL-6 and TNF-α in CMECs (P<0.05 vs. control). The CMECs after H/R injury had impaired cell viability (P<0.01 vs. control) and migration ability (P<0.05 vs. control). Moreover, the expressions of TLR4, NF-κB, IL-6 and TNF-α were elevated after H/R in CMECs (P<0.01 vs. control), while NO and the eNOS expression were significantly decreased. In contrast, administration of the TLR4-neutralizing antibody MTS510 prior to H/R injury down-regulated the expressions of IL-6 and TNF-α and attenuated the dysfunction of CMECs. Conclusion: TLR4 and its signaling components can be activated by LPS and H/R in CMECs. Blocking the TLR4 signal pathway before H/R injury attenuates CMEC dysfunction. Springer Basel AG, 2010 Toll-like receptor 4 nationallicence Cardiac microvascular endothelial cells nationallicence Lipopolysaccharide nationallicence Hypoxia/reoxygenation nationallicence Apoptosis nationallicence Zhang Zheng Department of Cardiology, Xijing Hospital, Fourth Military Medical University, 710032, Xi'an, Shaanxi, China aut Li Weijie Department of Cardiology, Xijing Hospital, Fourth Military Medical University, 710032, Xi'an, Shaanxi, China aut Sun Dongdong Department of Cardiology, Xijing Hospital, Fourth Military Medical University, 710032, Xi'an, Shaanxi, China aut Zhao Li Department of Cardiology, Xijing Hospital, Fourth Military Medical University, 710032, Xi'an, Shaanxi, China aut Zhang Rongqing Department of Cardiology, Xijing Hospital, Fourth Military Medical University, 710032, Xi'an, Shaanxi, China aut Wang Yabin Department of Cardiology, Xijing Hospital, Fourth Military Medical University, 710032, Xi'an, Shaanxi, China aut Zhou Xuan Department of Cardiology, Xijing Hospital, Fourth Military Medical University, 710032, Xi'an, Shaanxi, China aut Wang Haichang Department of Cardiology, Xijing Hospital, Fourth Military Medical University, 710032, Xi'an, Shaanxi, China aut Cao Feng Department of Cardiology, Xijing Hospital, Fourth Military Medical University, 710032, Xi'an, Shaanxi, China aut Inflammation Research SP Birkhäuser Verlag Basel 60/1(2011-01-01), 37-45 1023-3830 60:1<37 2011 60 11 https://doi.org/10.1007/s00011-010-0232-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00011-010-0232-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Zheng Department of Cardiology, Xijing Hospital, Fourth Military Medical University, 710032, Xi'an, Shaanxi, China aut NATIONALLICENCE 700 1- Li Weijie Department of Cardiology, Xijing Hospital, Fourth Military Medical University, 710032, Xi'an, Shaanxi, China aut NATIONALLICENCE 700 1- Sun Dongdong Department of Cardiology, Xijing Hospital, Fourth Military Medical University, 710032, Xi'an, Shaanxi, China aut NATIONALLICENCE 700 1- Zhao Li Department of Cardiology, Xijing Hospital, Fourth Military Medical University, 710032, Xi'an, Shaanxi, China aut NATIONALLICENCE 700 1- Zhang Rongqing Department of Cardiology, Xijing Hospital, Fourth Military Medical University, 710032, Xi'an, Shaanxi, China aut NATIONALLICENCE 700 1- Wang Yabin Department of Cardiology, Xijing Hospital, Fourth Military Medical University, 710032, Xi'an, Shaanxi, China aut NATIONALLICENCE 700 1- Zhou Xuan Department of Cardiology, Xijing Hospital, Fourth Military Medical University, 710032, Xi'an, Shaanxi, China aut NATIONALLICENCE 700 1- Wang Haichang Department of Cardiology, Xijing Hospital, Fourth Military Medical University, 710032, Xi'an, Shaanxi, China aut NATIONALLICENCE 700 1- Cao Feng Department of Cardiology, Xijing Hospital, Fourth Military Medical University, 710032, Xi'an, Shaanxi, China aut NATIONALLICENCE 773 0- Inflammation Research SP Birkhäuser Verlag Basel 60/1(2011-01-01), 37-45 1023-3830 60:1<37 2011 60 11 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer