caa a22 4500 445884800 CHVBK 20180317145555.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s00011-010-0234-0 doi (NATIONALLICENCE)springer-10.1007/s00011-010-0234-0 Up-regulation of histidine decarboxylase expression and histamine content in B16F10 murine melanoma cells [Elektronische Daten] [Steven Davis, Sheena Clark, J. Hayes, Todd Green, Carl Gruetter] Objective and design: This study was conducted to determine if differences in histidine decarboxylase expression and histamine levels exist between B16F10 melanoma cells and non-cancerous Melan-A melanocytes. Methods: Immunofluorescence and western blot analysis were used to detect and compare histidine decarboxylase protein levels. Enzyme-linked immunoassay was used to detect, measure, and compare histamine levels. Results: Histidine decarboxylase expression was found to be elevated in the B16F10 cells. Western blot analysis demonstrated levels of histidine decarboxylase protein expression more than twofold higher (p<0.001) in B16F10 than in Melan-A cells. Histamine levels were 280-fold higher (p<0.001) in B16F10 (229±15pg/mg protein) than in Melan-A (0.83±0.03pg/mg protein) cells. Conclusion: Results indicate an up-regulated histaminergic system in the B16F10 melanoma cells when compared to non-cancerous melanocytes. This supports the use of B16F10 cells as a model in which to investigate a potential role of the endogenous histaminergic system in regulating malignant cell function. Springer Basel AG, 2010 Histamine nationallicence B16F10 nationallicence Histidine decarboxylase nationallicence Melanocyte nationallicence Melanoma nationallicence Davis Steven Department of Pharmacology, Joan C. Edwards School of Medicine, Marshall University, 25704-9388, Huntington, WV, USA aut Clark Sheena Department of Pharmacology, Joan C. Edwards School of Medicine, Marshall University, 25704-9388, Huntington, WV, USA aut Hayes J. Department of Pharmacology, Joan C. Edwards School of Medicine, Marshall University, 25704-9388, Huntington, WV, USA aut Green Todd Department of Pharmacology, Joan C. Edwards School of Medicine, Marshall University, 25704-9388, Huntington, WV, USA aut Gruetter Carl Department of Pharmacology, Joan C. Edwards School of Medicine, Marshall University, 25704-9388, Huntington, WV, USA aut Inflammation Research SP Birkhäuser Verlag Basel 60/1(2011-01-01), 55-61 1023-3830 60:1<55 2011 60 11 https://doi.org/10.1007/s00011-010-0234-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00011-010-0234-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Davis Steven Department of Pharmacology, Joan C. Edwards School of Medicine, Marshall University, 25704-9388, Huntington, WV, USA aut NATIONALLICENCE 700 1- Clark Sheena Department of Pharmacology, Joan C. Edwards School of Medicine, Marshall University, 25704-9388, Huntington, WV, USA aut NATIONALLICENCE 700 1- Hayes J. Department of Pharmacology, Joan C. Edwards School of Medicine, Marshall University, 25704-9388, Huntington, WV, USA aut NATIONALLICENCE 700 1- Green Todd Department of Pharmacology, Joan C. Edwards School of Medicine, Marshall University, 25704-9388, Huntington, WV, USA aut NATIONALLICENCE 700 1- Gruetter Carl Department of Pharmacology, Joan C. Edwards School of Medicine, Marshall University, 25704-9388, Huntington, WV, USA aut NATIONALLICENCE 773 0- Inflammation Research SP Birkhäuser Verlag Basel 60/1(2011-01-01), 55-61 1023-3830 60:1<55 2011 60 11 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer