caa a22 4500 445884827 CHVBK 20180317145555.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s00011-010-0237-x doi (NATIONALLICENCE)springer-10.1007/s00011-010-0237-x Chlamydia trachomatis heat shock proteins 60 and 10 induce apoptosis in endocervical epithelial cells [Elektronische Daten] [Rajneesh Jha, Harsh Vardhan, Sylvette Bas, Sudha Salhan, Aruna Mittal] Aim and objective: The potential role of chlamydial heat shock proteins (cHSP) 60 and cHSP10 in apoptosis of primary cervical epithelial cells was investigated. Methods: Primary cervical epithelial cells were stimulated with cHSP60 and cHSP10 for 4h. Quantitative measurements of apoptosis were made using cytofluorometry, and apoptosis-related genes were analyzed by microarray, real-time PCR and western blotting. Further, levels of proinflammatory cytokines (IL-18 and IL-1β) were determined by semi-quantitative RT-PCR. Results: After a 4-h incubation in the presence of recombinant cHSP60 or cHSP10, the number of cells exhibiting annexin V binding activity increased 6- and 5-fold, respectively (P<0.05). A DNA microarray study showed significant (P<0.05) upregulation of interleukin (IL)-1 β-convertase, and caspase-3, -8 and -9 genes in cHSP60- and cHSP10-stimulated than in control cells as confirmed by real-time RT-PCR and western blotting. Transcript levels of IL-1β and IL-18 in cells treated with cHSP60 and cHSP10 were found to be significantly (P<0.05) higher in stimulated than in control cells. Conclusion: cHSP60- and cHSP10-induced caspase expression, proinflammatory cytokine production and apoptosis of primary cervical epithelial cells might play a role in the pathogenesis of infertility in women with persistent chlamydial infection. Springer Basel AG, 2010 Chlamydia trachomatis nationallicence cHSP60 nationallicence cHSP10 nationallicence Apoptosis nationallicence Epithelial cells nationallicence Jha Rajneesh Institute of Pathology, ICMR, Safdarjung Hospital Campus, Post Box no. 4909, 110 029, New Delhi, India aut Vardhan Harsh Institute of Pathology, ICMR, Safdarjung Hospital Campus, Post Box no. 4909, 110 029, New Delhi, India aut Bas Sylvette Genetics, Internal Medicine and Laboratory Medicine Department, University Hospital, 1211, Geneva 14, Switzerland aut Salhan Sudha Department of Gynecology and Obstetrics, Safdarjung Hospital, 110 029, New Delhi, India aut Mittal Aruna Institute of Pathology, ICMR, Safdarjung Hospital Campus, Post Box no. 4909, 110 029, New Delhi, India aut Inflammation Research SP Birkhäuser Verlag Basel 60/1(2011-01-01), 69-78 1023-3830 60:1<69 2011 60 11 https://doi.org/10.1007/s00011-010-0237-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00011-010-0237-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jha Rajneesh Institute of Pathology, ICMR, Safdarjung Hospital Campus, Post Box no. 4909, 110 029, New Delhi, India aut NATIONALLICENCE 700 1- Vardhan Harsh Institute of Pathology, ICMR, Safdarjung Hospital Campus, Post Box no. 4909, 110 029, New Delhi, India aut NATIONALLICENCE 700 1- Bas Sylvette Genetics, Internal Medicine and Laboratory Medicine Department, University Hospital, 1211, Geneva 14, Switzerland aut NATIONALLICENCE 700 1- Salhan Sudha Department of Gynecology and Obstetrics, Safdarjung Hospital, 110 029, New Delhi, India aut NATIONALLICENCE 700 1- Mittal Aruna Institute of Pathology, ICMR, Safdarjung Hospital Campus, Post Box no. 4909, 110 029, New Delhi, India aut NATIONALLICENCE 773 0- Inflammation Research SP Birkhäuser Verlag Basel 60/1(2011-01-01), 69-78 1023-3830 60:1<69 2011 60 11 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer