caa a22 4500 445884886 CHVBK 20180317145556.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s00011-010-0230-4 doi (NATIONALLICENCE)springer-10.1007/s00011-010-0230-4 IgE-dependent sensitization increases responsiveness to LPS but does not modify development of endotoxin tolerance in mast cells [Elektronische Daten] [Jaciel Medina-Tamayo, Alfredo Ibarra-Sánchez, Alejandro Padilla-Trejo, Claudia González-Espinosa] Objective: Effects of immunoglobulin E (IgE)-dependent sensitization on the response to bacterial lipopolysaccharide (LPS) were analyzed in mast cells. Methods: Murine bone marrow-derived mast cells (BMMCs) were sensitized or not with IgE before stimulation with LPS. TLR4 and co-receptors expression was analyzed by flow cytometry and RT-PCR, TNF-α production by ELISA, IKK and IκB activation by western blot or immunoprecipitation. NFκB nuclear translocation was determined by EMSA. Results: IgE-sensitized BMMCs secreted larger amounts of TNF-α than non-sensitized cells shortly after LPS challenge. No change in TLR4, CD14 or MD-2 expression was detected after the IgE-dependent sensitization process, whereas TLR4-dependent phosphorylation of IKK and IκB was augmented. IgE-dependent sensitization increased basal NFκB activity. Endotoxin tolerance was not affected by the IgE-dependent sensitization process. Conclusions: IgE-induced sensitization primes mast cells for higher response to LPS through pre-activation of NFκB transcription factor. IgE-dependent sensitization does not modify events leading to endotoxin tolerance. Springer Basel AG, 2010 Mast cell nationallicence IgE nationallicence FcεRI nationallicence TLR4 nationallicence Endotoxin tolerance nationallicence Medina-Tamayo Jaciel Departamento de Farmacobiología, Centro de Investigación y de Estudios Avanzados del IPN, Sede Sur, Calzada de los Tenorios No. 235, Col. Granjas Coapa, Tlalpan, Mexico, D.F., México aut Ibarra-Sánchez Alfredo Departamento de Farmacobiología, Centro de Investigación y de Estudios Avanzados del IPN, Sede Sur, Calzada de los Tenorios No. 235, Col. Granjas Coapa, Tlalpan, Mexico, D.F., México aut Padilla-Trejo Alejandro Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico, México aut González-Espinosa Claudia Departamento de Farmacobiología, Centro de Investigación y de Estudios Avanzados del IPN, Sede Sur, Calzada de los Tenorios No. 235, Col. Granjas Coapa, Tlalpan, Mexico, D.F., México aut Inflammation Research SP Birkhäuser Verlag Basel 60/1(2011-01-01), 19-27 1023-3830 60:1<19 2011 60 11 https://doi.org/10.1007/s00011-010-0230-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00011-010-0230-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Medina-Tamayo Jaciel Departamento de Farmacobiología, Centro de Investigación y de Estudios Avanzados del IPN, Sede Sur, Calzada de los Tenorios No. 235, Col. Granjas Coapa, Tlalpan, Mexico, D.F., México aut NATIONALLICENCE 700 1- Ibarra-Sánchez Alfredo Departamento de Farmacobiología, Centro de Investigación y de Estudios Avanzados del IPN, Sede Sur, Calzada de los Tenorios No. 235, Col. Granjas Coapa, Tlalpan, Mexico, D.F., México aut NATIONALLICENCE 700 1- Padilla-Trejo Alejandro Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico, México aut NATIONALLICENCE 700 1- González-Espinosa Claudia Departamento de Farmacobiología, Centro de Investigación y de Estudios Avanzados del IPN, Sede Sur, Calzada de los Tenorios No. 235, Col. Granjas Coapa, Tlalpan, Mexico, D.F., México aut NATIONALLICENCE 773 0- Inflammation Research SP Birkhäuser Verlag Basel 60/1(2011-01-01), 19-27 1023-3830 60:1<19 2011 60 11 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer