caa a22 4500 445884908 CHVBK 20180317145556.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s00011-010-0238-9 doi (NATIONALLICENCE)springer-10.1007/s00011-010-0238-9 Human skin penetration of a copper tripeptide in vitro as a function of skin layer [Elektronische Daten] [Jurij Hostynek, Frank Dreher, Howard Maibach] Objective and design: Skin retention and penetration by copper applied as glycyl-l-histidyl-l-lysine cuprate diacetate was evaluated in vitro in order to assess its potential for its transdermal delivery as an anti-inflammatory agent. Materials and methods: Flow-through diffusion cells with 1cm2 exposure area were used under infinite dose conditions.0.68% aq. copper tripeptide as permeant was applied on isolated stratum corneum, heat-separated epidermis and dermatomed skin and receptor fluid collected over 48h in 4h intervals using inductively coupled plasma mass spectrometry to analyze for copper in tissues and receptor fluid. Results: The permeability coefficient of the compound through dermatomed skin was 2.43±0.51×10−4 cm/h; 136.2±17.5μg/cm2 copper permeated 1cm2 of that tissue over 48h, while 97±6.6μg/cm2 were retained as depot. Conclusions: Copper as tripeptide was delivered in potentially therapeutically effective amounts for inflammatory disease. The Author(s), 2010 Absorption nationallicence Arthritis nationallicence Bioavailability nationallicence Copper complex nationallicence Depot nationallicence Inflammation nationallicence Hostynek Jurij Department of Dermatology, UCSF School of Medicine, San Francisco, CA, USA aut Dreher Frank Department of Dermatology, UCSF School of Medicine, San Francisco, CA, USA aut Maibach Howard Department of Dermatology, UCSF School of Medicine, San Francisco, CA, USA aut Inflammation Research SP Birkhäuser Verlag Basel 60/1(2011-01-01), 79-86 1023-3830 60:1<79 2011 60 11 https://doi.org/10.1007/s00011-010-0238-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00011-010-0238-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hostynek Jurij Department of Dermatology, UCSF School of Medicine, San Francisco, CA, USA aut NATIONALLICENCE 700 1- Dreher Frank Department of Dermatology, UCSF School of Medicine, San Francisco, CA, USA aut NATIONALLICENCE 700 1- Maibach Howard Department of Dermatology, UCSF School of Medicine, San Francisco, CA, USA aut NATIONALLICENCE 773 0- Inflammation Research SP Birkhäuser Verlag Basel 60/1(2011-01-01), 79-86 1023-3830 60:1<79 2011 60 11 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer