caa a22 4500 445884932 CHVBK 20180317145556.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s00011-010-0233-1 doi (NATIONALLICENCE)springer-10.1007/s00011-010-0233-1 Intrahepatic levels of PD-1/PD-L correlate with liver inflammation in chronic hepatitis B [Elektronische Daten] [Ji Chen, Xiao-Mei Wang, Xue-Jie Wu, Yan Wang, Hong Zhao, Bing Shen, Gui-Qiang Wang] Background and objective: Programed cell death-1 (PD-1) represents a mechanism of T-cell dysfunction in hepatitis B virus (HBV) persistence. In peripheral blood, PD-1 is up-regulated in virus-specific T cells, leading to the impairment of T cells. This study investigated the intrahepatic expression of PD-1 and its ligand (PD-L) in patients with chronic hepatitis B (CHB) virus. Methods: Liver specimens were obtained from CHB (n=56), acute hepatitis B (AHB, n=12) patients and age-matched healthy subjects (n=10). The expression of PD-1/PD-L was determined by immunohistochemistry. Results: In CHB patients, PD-1 was predominantly expressed in lymphocytes infiltrating the portal tract. PD-L1 was detected in lymphocytes, hepatocytes and liver sinusoidal endothelial cells, while PD-L2 was localized in Kupffer cells and dendritic cells. The labeling indexes of PD-1 and PD-L1 in lymphocytes infiltrating portal area were significantly higher in CHB patients than in healthy controls and AHB patients. Within the CHB patients, the increases in labeling indexes of PD-1 and PD-L paralleled the degree of inflammation. Conclusions: These results suggest that over-expression of PD-1, PD-L1 and PD-L2 within liver may participate in local immune dysfunction, which could be one of the mechanisms involved in the chronicity of HBV infection and chronic inflammation seen in CHB patients. Springer Basel AG, 2010 Chronic hepatitis B nationallicence PD-1/PD-L nationallicence Intrahepatic protein expression nationallicence Inflammation nationallicence Chen Ji Department of Infectious Diseases, Peking University First Hospital, No. 8, Xishiku Street, Xicheng District, 100034, Beijing, People's Republic of China aut Wang Xiao-Mei Department of Pathology, Beijing Ditan Hospital, No. 8, Jingshun East Road, Chaoyang District, 100015, Beijing, People's Republic of China aut Wu Xue-Jie Department of Infectious Diseases, Peking University First Hospital, No. 8, Xishiku Street, Xicheng District, 100034, Beijing, People's Republic of China aut Wang Yan Department of Infectious Diseases, Peking University First Hospital, No. 8, Xishiku Street, Xicheng District, 100034, Beijing, People's Republic of China aut Zhao Hong Department of Infectious Diseases, Peking University First Hospital, No. 8, Xishiku Street, Xicheng District, 100034, Beijing, People's Republic of China aut Shen Bing Department of Pathology, Beijing Ditan Hospital, No. 8, Jingshun East Road, Chaoyang District, 100015, Beijing, People's Republic of China aut Wang Gui-Qiang Department of Infectious Diseases, Peking University First Hospital, No. 8, Xishiku Street, Xicheng District, 100034, Beijing, People's Republic of China aut Inflammation Research SP Birkhäuser Verlag Basel 60/1(2011-01-01), 47-53 1023-3830 60:1<47 2011 60 11 https://doi.org/10.1007/s00011-010-0233-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00011-010-0233-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chen Ji Department of Infectious Diseases, Peking University First Hospital, No. 8, Xishiku Street, Xicheng District, 100034, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Wang Xiao-Mei Department of Pathology, Beijing Ditan Hospital, No. 8, Jingshun East Road, Chaoyang District, 100015, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Wu Xue-Jie Department of Infectious Diseases, Peking University First Hospital, No. 8, Xishiku Street, Xicheng District, 100034, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Wang Yan Department of Infectious Diseases, Peking University First Hospital, No. 8, Xishiku Street, Xicheng District, 100034, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Zhao Hong Department of Infectious Diseases, Peking University First Hospital, No. 8, Xishiku Street, Xicheng District, 100034, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Shen Bing Department of Pathology, Beijing Ditan Hospital, No. 8, Jingshun East Road, Chaoyang District, 100015, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Wang Gui-Qiang Department of Infectious Diseases, Peking University First Hospital, No. 8, Xishiku Street, Xicheng District, 100034, Beijing, People's Republic of China aut NATIONALLICENCE 773 0- Inflammation Research SP Birkhäuser Verlag Basel 60/1(2011-01-01), 47-53 1023-3830 60:1<47 2011 60 11 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer