caa a22 4500 445884940 CHVBK 20180317145556.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s00011-010-0300-7 doi (NATIONALLICENCE)springer-10.1007/s00011-010-0300-7 Oxymatrine reduces neuronal cell apoptosis by inhibiting Toll-like receptor 4/nuclear factor kappa-B-dependent inflammatory responses in traumatic rat brain injury [Elektronische Daten] [Xiao-Qiao Dong, Wen-Hua Yu, Yue-Yu Hu, Zu-Yong Zhang, Man Huang] Objective: To investigate the influence of oxymatrine (OMT) on Toll-like receptor 4 (TLR-4)/nuclear factor kappa-B (NF-κB)-dependent inflammatory responses and neuronal cell apoptosis after traumatic brain injury (TBI). Materials and methods: Wistar rats were given an intraperitoneal injection of 60 or 120mg/kg OMT after TBI once a day till day 5. Rats were killed by decapitation at hours 2, 6 and 12, and days 1, 2, 3 and 5 after TBI. Gene expressions of TLR-4 and NF-κB, concentrations of tumor necrosis factor-alpha (TNF-α), interleukin-1beta (IL-1β) and interleukin-6 (IL-6) as well as the number of apoptotic neuronal cells in traumatic rat brain tissues were determined. Results: The administration of 120mg/kg OMT could significantly suppress gene expressions of TLR-4 and NF-κB, lessen concentrations of TNF-α, IL-1β and IL-6, and reduce the number of apoptotic neuronal cells in traumatic rat brain tissues by the Mann-Whitney U test (P<0.05), but the administration of 60mg/kg OMT could not (P>0.05). Conclusion: OMT may inhibit TLR4/NF-κB-dependent inflammatory responses, and furthermore lessen neuronal cell apoptosis after TBI. Springer Basel AG, 2010 Traumatic brain injury nationallicence Oxymatrine nationallicence Toll-like receptor 4 nationallicence Nuclear factor kappa-B nationallicence Inflammatory cytokine nationallicence Neuronal cell apoptosis nationallicence Dong Xiao-Qiao Department of Neurosurgery, The First Hangzhou Municipal People's Hospital, Nanjing Medical University, 261 Huansha Road, 310000, Hangzhou, China aut Yu Wen-Hua Department of Neurosurgery, The First Hangzhou Municipal People's Hospital, Nanjing Medical University, 261 Huansha Road, 310000, Hangzhou, China aut Hu Yue-Yu Department of Intensive Care Unit, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, 3 Qingchun East Road, 310000, Hangzhou, China aut Zhang Zu-Yong Department of Neurosurgery, The First Hangzhou Municipal People's Hospital, Nanjing Medical University, 261 Huansha Road, 310000, Hangzhou, China aut Huang Man Department of Intensive Care Unit, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, 3 Qingchun East Road, 310000, Hangzhou, China aut Inflammation Research SP Birkhäuser Verlag Basel 60/6(2011-06-01), 533-539 1023-3830 60:6<533 2011 60 11 https://doi.org/10.1007/s00011-010-0300-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00011-010-0300-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dong Xiao-Qiao Department of Neurosurgery, The First Hangzhou Municipal People's Hospital, Nanjing Medical University, 261 Huansha Road, 310000, Hangzhou, China aut NATIONALLICENCE 700 1- Yu Wen-Hua Department of Neurosurgery, The First Hangzhou Municipal People's Hospital, Nanjing Medical University, 261 Huansha Road, 310000, Hangzhou, China aut NATIONALLICENCE 700 1- Hu Yue-Yu Department of Intensive Care Unit, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, 3 Qingchun East Road, 310000, Hangzhou, China aut NATIONALLICENCE 700 1- Zhang Zu-Yong Department of Neurosurgery, The First Hangzhou Municipal People's Hospital, Nanjing Medical University, 261 Huansha Road, 310000, Hangzhou, China aut NATIONALLICENCE 700 1- Huang Man Department of Intensive Care Unit, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, 3 Qingchun East Road, 310000, Hangzhou, China aut NATIONALLICENCE 773 0- Inflammation Research SP Birkhäuser Verlag Basel 60/6(2011-06-01), 533-539 1023-3830 60:6<533 2011 60 11 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer