caa a22 4500 445884959 CHVBK 20180317145556.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s00011-010-0301-6 doi (NATIONALLICENCE)springer-10.1007/s00011-010-0301-6 Effects of endogenous substance P expression on degranulation in RBL-2H3 cells [Elektronische Daten] [Hua Hu, Ruxin Zhang, Xiaoyun Fang, Min Yu, Shaoqing Yu, Jie Zhang, Hong Wang] Objective and design: To determine whether RBL-2H3 cells have endogenous substance P (SP) expression under immunoglobulin E (IgE)-activated and inactivated conditions, and to ascertain the function of endogenous SP in the antigen-induced degranulation of RBL-2H3 cells. Materials and methods: SP mRNA and protein expression in both inactivated and 2,4-dinitrophenol (DNP)-specific IgE-activated RBL-2H3 cells were assessed by real-time PCR and immunofluorescence, respectively. Following activation with DNP-specific IgE, the degranulation of RBL-2H3 cells in response to DNP-bovine serum albumin (BSA), with and without endogenous SP expression, was assessed by monitoring the release of the granular enzyme β-hexosaminidase. Results: Endogenous SP mRNA and peptide expression increased in activated RBL-2H3 cells, compared with inactivated RBL-2H3 cells. The small hairpin RNA (shRNA)-mediated knockdown of endogenous SP reduced the degranulation ability of RBL-2H3 cells. Conclusions: Activated RBL-2H3 cells express endogenous SP which plays a role in antigen-induced degranulation. Springer Basel AG, 2010 Substance P nationallicence Mast cell nationallicence Neuroimmune nationallicence Degranulation nationallicence RNAi nationallicence Hu Hua Department of ENT, Huadong Hospital, Shanghai Medical School, Fudan University, 200032, Shanghai, People's Republic of China aut Zhang Ruxin Department of ENT, Huadong Hospital, Shanghai Medical School, Fudan University, 200032, Shanghai, People's Republic of China aut Fang Xiaoyun Department of Biochemistry and Molecular Biology & The Key Laboratory of Molecular Medicine, Ministry of Education, Shanghai Medical School, Fudan University, 200032, Shanghai, People's Republic of China aut Yu Min Department of Biochemistry and Molecular Biology & The Key Laboratory of Molecular Medicine, Ministry of Education, Shanghai Medical School, Fudan University, 200032, Shanghai, People's Republic of China aut Yu Shaoqing Department of ENT, Huadong Hospital, Shanghai Medical School, Fudan University, 200032, Shanghai, People's Republic of China aut Zhang Jie Department of Clinical Medicine, Shanghai JiaoTong University School of Medicine, 200025, Shanghai, People's Republic of China aut Wang Hong Department of ENT, Huadong Hospital, Shanghai Medical School, Fudan University, 200032, Shanghai, People's Republic of China aut Inflammation Research SP Birkhäuser Verlag Basel 60/6(2011-06-01), 541-546 1023-3830 60:6<541 2011 60 11 https://doi.org/10.1007/s00011-010-0301-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00011-010-0301-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hu Hua Department of ENT, Huadong Hospital, Shanghai Medical School, Fudan University, 200032, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Ruxin Department of ENT, Huadong Hospital, Shanghai Medical School, Fudan University, 200032, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Fang Xiaoyun Department of Biochemistry and Molecular Biology & The Key Laboratory of Molecular Medicine, Ministry of Education, Shanghai Medical School, Fudan University, 200032, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Yu Min Department of Biochemistry and Molecular Biology & The Key Laboratory of Molecular Medicine, Ministry of Education, Shanghai Medical School, Fudan University, 200032, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Yu Shaoqing Department of ENT, Huadong Hospital, Shanghai Medical School, Fudan University, 200032, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Jie Department of Clinical Medicine, Shanghai JiaoTong University School of Medicine, 200025, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Wang Hong Department of ENT, Huadong Hospital, Shanghai Medical School, Fudan University, 200032, Shanghai, People's Republic of China aut NATIONALLICENCE 773 0- Inflammation Research SP Birkhäuser Verlag Basel 60/6(2011-06-01), 541-546 1023-3830 60:6<541 2011 60 11 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer