caa a22 4500 445885009 CHVBK 20180317145556.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s00011-011-0327-4 doi (NATIONALLICENCE)springer-10.1007/s00011-011-0327-4 Adjuvant arthritis influences expression of housekeeping genes [Elektronische Daten] [Sherif Hanafy, Fakhreddin Jamali] Objective and method: We determined whether adjuvant arthritis (AA) affects expression of three housekeeping (HK) genes, glyceraldehyde-3-phosphate-dehydrogenase (GAPDH), β-actin and 18s ribosomal RNA (18s rRNA) in organs of Sprague-Dawley rats using the real-time reverse-transcription polymerase chain reaction (RT-PCR). Results: Expression of 18s rRNA was unaffected by AA whereas that of β-actin was reduced by 52, 62 and 81% in the liver, kidney and intestine, respectively, but not in the heart. AA significantly reduced GAPDH expression in the intestine. Conclusion: For reliable results, the HK gene must be carefully selected. Springer Basel AG, 2011 Polymerase chain reaction nationallicence Adjuvant arthritis nationallicence HK genes nationallicence Hanafy Sherif Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, T6G 2N8, Edmonton, AB, Canada aut Jamali Fakhreddin Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, T6G 2N8, Edmonton, AB, Canada aut Inflammation Research SP Birkhäuser Verlag Basel 60/6(2011-06-01), 521-523 1023-3830 60:6<521 2011 60 11 https://doi.org/10.1007/s00011-011-0327-4 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00011-011-0327-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hanafy Sherif Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, T6G 2N8, Edmonton, AB, Canada aut NATIONALLICENCE 700 1- Jamali Fakhreddin Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, T6G 2N8, Edmonton, AB, Canada aut NATIONALLICENCE 773 0- Inflammation Research SP Birkhäuser Verlag Basel 60/6(2011-06-01), 521-523 1023-3830 60:6<521 2011 60 11 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer