caa a22 4500 445885017 CHVBK 20180317145556.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s00011-010-0305-2 doi (NATIONALLICENCE)springer-10.1007/s00011-010-0305-2 Urinary trypsin inhibitor attenuates lipopolysaccharide-induced acute lung injury by blocking the activation of p38 mitogen-activated protein kinase [Elektronische Daten] [Xinying Zhang, Fengqin Liu, Haiyan Liu, Hongxia Cheng, Wei Wang, Qiang Wen, Yulin Wang] Objective: To investigate the protective effect of urinary trypsin inhibitor (UTI) in a rat model of lipopolysaccharide (LPS)-induced acute lung injury (ALI) and the underlying molecular mechanism. Methods: Rats were randomly assigned into three groups: control group, LPS treatment group and LPS/UTI treatment group. The serum concentrations of tumor necrosis factor (TNF)-α and interleukin (IL)-10 were measured by ELISA. The expression of p38 mitogen-activated protein kinase (MAPK) in lung tissues was determined by Western blot analysis. Results: Administration of UTI reduced the lung wet/dry weight ratio and ameliorated the tissue damage. In the LPS/UTI treatment group, levels of TNF-α were significantly lower than those in the LPS treatment group, while the levels of IL-10 were significantly higher than those in the LPS treatment group. Western blot analysis revealed that UTI inhibited the phosphorylation of p38 MAPK in lung tissues. Conclusions: UTI attenuates LPS-induced ALI, probably by adjusting the balance between proinflammatory and anti-inflammatory cytokines. The mechanism responsible for the decreased TNF-α expression may be related to the inhibitory effect of UTI on p38 MAPK activation. Springer Basel AG, 2011 Urinary trypsin inhibitor nationallicence Lipopolysaccharide nationallicence Acute lung injury nationallicence Tumor necrosis factor-α nationallicence Interleukin-10 nationallicence p38 Mitogen-activated protein kinase nationallicence Zhang Xinying Department of Pediatrics, Provincial Hospital Affiliated to Shandong University, 324 Jingwuweiqi Road, 250021, Jinan, China aut Liu Fengqin Department of Pediatrics, Provincial Hospital Affiliated to Shandong University, 324 Jingwuweiqi Road, 250021, Jinan, China aut Liu Haiyan Department of Pediatrics, Provincial Hospital Affiliated to Shandong University, 324 Jingwuweiqi Road, 250021, Jinan, China aut Cheng Hongxia Department of Pathology, Provincial Hospital Affiliated to Shandong University, Jinan, China aut Wang Wei Department of Pediatrics, Provincial Hospital Affiliated to Shandong University, 324 Jingwuweiqi Road, 250021, Jinan, China aut Wen Qiang Department of Clinical Medicine, Weifang Medical College, Weifang, China aut Wang Yulin Department of Pediatrics, Provincial Hospital Affiliated to Shandong University, 324 Jingwuweiqi Road, 250021, Jinan, China aut Inflammation Research SP Birkhäuser Verlag Basel 60/6(2011-06-01), 569-575 1023-3830 60:6<569 2011 60 11 https://doi.org/10.1007/s00011-010-0305-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00011-010-0305-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Xinying Department of Pediatrics, Provincial Hospital Affiliated to Shandong University, 324 Jingwuweiqi Road, 250021, Jinan, China aut NATIONALLICENCE 700 1- Liu Fengqin Department of Pediatrics, Provincial Hospital Affiliated to Shandong University, 324 Jingwuweiqi Road, 250021, Jinan, China aut NATIONALLICENCE 700 1- Liu Haiyan Department of Pediatrics, Provincial Hospital Affiliated to Shandong University, 324 Jingwuweiqi Road, 250021, Jinan, China aut NATIONALLICENCE 700 1- Cheng Hongxia Department of Pathology, Provincial Hospital Affiliated to Shandong University, Jinan, China aut NATIONALLICENCE 700 1- Wang Wei Department of Pediatrics, Provincial Hospital Affiliated to Shandong University, 324 Jingwuweiqi Road, 250021, Jinan, China aut NATIONALLICENCE 700 1- Wen Qiang Department of Clinical Medicine, Weifang Medical College, Weifang, China aut NATIONALLICENCE 700 1- Wang Yulin Department of Pediatrics, Provincial Hospital Affiliated to Shandong University, 324 Jingwuweiqi Road, 250021, Jinan, China aut NATIONALLICENCE 773 0- Inflammation Research SP Birkhäuser Verlag Basel 60/6(2011-06-01), 569-575 1023-3830 60:6<569 2011 60 11 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer