caa a22 4500 445885025 CHVBK 20180317145556.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s00011-011-0309-6 doi (NATIONALLICENCE)springer-10.1007/s00011-011-0309-6 Reactive oxygen species and NADPH oxidase 4 induced by transforming growth factor β1 are the therapeutic targets of polyenylphosphatidylcholine in the suppression of human hepatic stellate cell activation [Elektronische Daten] [Remina Ikeda, Kyoko Ishii, Yoshiko Hoshikawa, Junya Azumi, Yuta Arakaki, Toshihiro Yasui, Shizuka Matsuura, Yoshiaki Matsumi, Yohei Kono, Yusuke Mizuta, Akihiro Kurimasa, Ichiro Hisatome, Scott Friedman, Hironaka Kawasaki, Goshi Shiota] Objective and design: To clarify the molecular mechanism of polyenylphosphatidylcholine (PPC), we examined the involvement of reactive oxygen species (ROS) and NADPH oxidase 4 (Nox4) in human hepatic stellate cells (HSCs). Material: Using human LX-2 HSC cells, we examined the effects of PPC on expression of α-smooth muscle actin (α-SMA) and collagen 1, generation of ROS, Nox4 expression, p38 activation and cell proliferation, induced by transforming growth factor β1 (TGFβ1). Results: PPC suppressed ROS which are induced by TGFβ1, phosphorylation of p38MAPK, and expression levels of α-SMA and collagen 1 in a dose-dependent manner. Higher concentrations of PPC also suppressed Nox4 levels. Conclusion: These results suggest that ROS and Nox4 induced by TGFβ1 are the therapeutic targets of PPC in the suppression of human hepatic stellate cell activation. Springer Basel AG, 2011 Polyenylphosphatidylcholine nationallicence NADPH oxidase 4 nationallicence Hepatic stellate cells nationallicence Oxidative stress nationallicence PPC : Polyenylphosphatidylcholine nationallicence HSC : Hepatic stellate cell nationallicence Nox : NADPH oxidase nationallicence MFB : Myofibroblast nationallicence PDGF : Platelet-derived growth factor nationallicence TGFβ1 : Transforming growth factor β-1 nationallicence ECM : Extracellular matrix nationallicence HCC : Hepatocellular carcinoma nationallicence LC : Liver cirrhosis nationallicence Ikeda Remina Division of Molecular and Genetic Medicine, Department of Genetic Medicine and Regenerative Therapeutics, Graduate School of Medicine, Tottori University, 683-8504, Yonago, Japan aut Ishii Kyoko Division of Molecular and Genetic Medicine, Department of Genetic Medicine and Regenerative Therapeutics, Graduate School of Medicine, Tottori University, 683-8504, Yonago, Japan aut Hoshikawa Yoshiko Division of Molecular and Genetic Medicine, Department of Genetic Medicine and Regenerative Therapeutics, Graduate School of Medicine, Tottori University, 683-8504, Yonago, Japan aut Azumi Junya Division of Molecular and Genetic Medicine, Department of Genetic Medicine and Regenerative Therapeutics, Graduate School of Medicine, Tottori University, 683-8504, Yonago, Japan aut Arakaki Yuta Division of Molecular and Genetic Medicine, Department of Genetic Medicine and Regenerative Therapeutics, Graduate School of Medicine, Tottori University, 683-8504, Yonago, Japan aut Yasui Toshihiro Division of Molecular and Genetic Medicine, Department of Genetic Medicine and Regenerative Therapeutics, Graduate School of Medicine, Tottori University, 683-8504, Yonago, Japan aut Matsuura Shizuka Division of Molecular and Genetic Medicine, Department of Genetic Medicine and Regenerative Therapeutics, Graduate School of Medicine, Tottori University, 683-8504, Yonago, Japan aut Matsumi Yoshiaki Division of Molecular and Genetic Medicine, Department of Genetic Medicine and Regenerative Therapeutics, Graduate School of Medicine, Tottori University, 683-8504, Yonago, Japan aut Kono Yohei Division of Molecular and Genetic Medicine, Department of Genetic Medicine and Regenerative Therapeutics, Graduate School of Medicine, Tottori University, 683-8504, Yonago, Japan aut Mizuta Yusuke Division of Molecular and Genetic Medicine, Department of Genetic Medicine and Regenerative Therapeutics, Graduate School of Medicine, Tottori University, 683-8504, Yonago, Japan aut Kurimasa Akihiro Division of Molecular and Genetic Medicine, Department of Genetic Medicine and Regenerative Therapeutics, Graduate School of Medicine, Tottori University, 683-8504, Yonago, Japan aut Hisatome Ichiro Division of Regenerative Therapeutics, Department of Genetic Medicine and Regenerative Therapeutics, Graduate School of Medicine, Tottori University, Yonago, Japan aut Friedman Scott Division of Liver Diseases, Mount Sinai School of Medicine, New York, USA aut Kawasaki Hironaka Department of Internal Medicine, San-in Rosai Hospital, Yonago, Japan aut Shiota Goshi Division of Molecular and Genetic Medicine, Department of Genetic Medicine and Regenerative Therapeutics, Graduate School of Medicine, Tottori University, 683-8504, Yonago, Japan aut Inflammation Research SP Birkhäuser Verlag Basel 60/6(2011-06-01), 597-604 1023-3830 60:6<597 2011 60 11 https://doi.org/10.1007/s00011-011-0309-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00011-011-0309-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ikeda Remina Division of Molecular and Genetic Medicine, Department of Genetic Medicine and Regenerative Therapeutics, Graduate School of Medicine, Tottori University, 683-8504, Yonago, Japan aut NATIONALLICENCE 700 1- Ishii Kyoko Division of Molecular and Genetic Medicine, Department of Genetic Medicine and Regenerative Therapeutics, Graduate School of Medicine, Tottori University, 683-8504, Yonago, Japan aut NATIONALLICENCE 700 1- Hoshikawa Yoshiko Division of Molecular and Genetic Medicine, Department of Genetic Medicine and Regenerative Therapeutics, Graduate School of Medicine, Tottori University, 683-8504, Yonago, Japan aut NATIONALLICENCE 700 1- Azumi Junya Division of Molecular and Genetic Medicine, Department of Genetic Medicine and Regenerative Therapeutics, Graduate School of Medicine, Tottori University, 683-8504, Yonago, Japan aut NATIONALLICENCE 700 1- Arakaki Yuta Division of Molecular and Genetic Medicine, Department of Genetic Medicine and Regenerative Therapeutics, Graduate School of Medicine, Tottori University, 683-8504, Yonago, Japan aut NATIONALLICENCE 700 1- Yasui Toshihiro Division of Molecular and Genetic Medicine, Department of Genetic Medicine and Regenerative Therapeutics, Graduate School of Medicine, Tottori University, 683-8504, Yonago, Japan aut NATIONALLICENCE 700 1- Matsuura Shizuka Division of Molecular and Genetic Medicine, Department of Genetic Medicine and Regenerative Therapeutics, Graduate School of Medicine, Tottori University, 683-8504, Yonago, Japan aut NATIONALLICENCE 700 1- Matsumi Yoshiaki Division of Molecular and Genetic Medicine, Department of Genetic Medicine and Regenerative Therapeutics, Graduate School of Medicine, Tottori University, 683-8504, Yonago, Japan aut NATIONALLICENCE 700 1- Kono Yohei Division of Molecular and Genetic Medicine, Department of Genetic Medicine and Regenerative Therapeutics, Graduate School of Medicine, Tottori University, 683-8504, Yonago, Japan aut NATIONALLICENCE 700 1- Mizuta Yusuke Division of Molecular and Genetic Medicine, Department of Genetic Medicine and Regenerative Therapeutics, Graduate School of Medicine, Tottori University, 683-8504, Yonago, Japan aut NATIONALLICENCE 700 1- Kurimasa Akihiro Division of Molecular and Genetic Medicine, Department of Genetic Medicine and Regenerative Therapeutics, Graduate School of Medicine, Tottori University, 683-8504, Yonago, Japan aut NATIONALLICENCE 700 1- Hisatome Ichiro Division of Regenerative Therapeutics, Department of Genetic Medicine and Regenerative Therapeutics, Graduate School of Medicine, Tottori University, Yonago, Japan aut NATIONALLICENCE 700 1- Friedman Scott Division of Liver Diseases, Mount Sinai School of Medicine, New York, USA aut NATIONALLICENCE 700 1- Kawasaki Hironaka Department of Internal Medicine, San-in Rosai Hospital, Yonago, Japan aut NATIONALLICENCE 700 1- Shiota Goshi Division of Molecular and Genetic Medicine, Department of Genetic Medicine and Regenerative Therapeutics, Graduate School of Medicine, Tottori University, 683-8504, Yonago, Japan aut NATIONALLICENCE 773 0- Inflammation Research SP Birkhäuser Verlag Basel 60/6(2011-06-01), 597-604 1023-3830 60:6<597 2011 60 11 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer