caa a22 4500 445885068 CHVBK 20180317145556.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s00011-010-0304-3 doi (NATIONALLICENCE)springer-10.1007/s00011-010-0304-3 Atherosclerosis aggravates ischemia/reperfusion injury in the gut and remote damage in the liver and the lung [Elektronische Daten] [René Schramm, Frank Appel, Manfred Reinacher, Hans-Joachim Schäfers, Benjamin Bierbach, Jan Slotta, Henrik Thorlacius, Michael Menger] Objective: We investigated whether mesenteric ischemia/reperfusion (I/R)-associated gut injury and remote liver and lung damage are affected by prevalent atherosclerosis. Methods: Mesenteric ischemia was induced in atherosclerotic ApoE-deficient (ApoE−/−) and control C57BL/6 mice by clamping the superior mesenteric artery for 30min. Mesenteric microcirculatory dysfunction and leukocytic inflammation were studied in the terminal ileum by intravital fluorescence microscopy (IVM). Histological analyses included quantitative assessment of parenchymal injury in the terminal ileum, liver and lung. Results: In the gut, IVM of the terminal ileum revealed aggravated postischemic microcirculatory dysfunction and absence of reactive hyperemia-induced vasodilation in atherosclerotic mice compared to controls. In addition, leukocyte-endothelial cell adhesive interactions, i.e. rolling and firm adhesion, were significantly increased in atherosclerotic animals. This was associated with enhanced mucosal tissue damage in ApoE−/− mice. Moreover, mesenteric I/R-provoked remote parenchymal injury in the liver was found to be significantly aggravated in atherosclerotic mice. This was accompanied by enhanced neutrophilic lung inflammation in ApoE−/− mice. Conclusion: Prevalent generalized atherosclerosis not only aggravates splanchnic microcirculatory dysfunction and leukocytic inflammation in response to mesenteric I/R, but also exacerbates mucosal tissue damage and remote injury in the liver and the lung. Springer Basel AG, 2011 Atherosclerosis nationallicence Ischemia/reperfusion nationallicence GI inflammation nationallicence In vivo inflammation nationallicence Intravital microscopy nationallicence Schramm René Clinic of Cardiac Surgery, Ludwig-Maximilians-University of Munich-Grosshadern, Marchioninistr.15, 81377, Munich, Germany aut Appel Frank Institute for Clinical and Experimental Surgery, University of Saarland, Homburg, Germany aut Reinacher Manfred Institute for Veterinary Pathology, University of Giessen, Giessen, Germany aut Schäfers Hans-Joachim Clinic for Thoracic- and Cardiovascular Surgery, University of Saarland, Homburg, Germany aut Bierbach Benjamin Clinic for Thoracic- and Cardiovascular Surgery, University of Saarland, Homburg, Germany aut Slotta Jan Institute for Clinical and Experimental Surgery, University of Saarland, Homburg, Germany aut Thorlacius Henrik Department of Surgery, Lund University, Lund, Sweden aut Menger Michael Institute for Clinical and Experimental Surgery, University of Saarland, Homburg, Germany aut Inflammation Research SP Birkhäuser Verlag Basel 60/6(2011-06-01), 555-567 1023-3830 60:6<555 2011 60 11 https://doi.org/10.1007/s00011-010-0304-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00011-010-0304-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Schramm René Clinic of Cardiac Surgery, Ludwig-Maximilians-University of Munich-Grosshadern, Marchioninistr.15, 81377, Munich, Germany aut NATIONALLICENCE 700 1- Appel Frank Institute for Clinical and Experimental Surgery, University of Saarland, Homburg, Germany aut NATIONALLICENCE 700 1- Reinacher Manfred Institute for Veterinary Pathology, University of Giessen, Giessen, Germany aut NATIONALLICENCE 700 1- Schäfers Hans-Joachim Clinic for Thoracic- and Cardiovascular Surgery, University of Saarland, Homburg, Germany aut NATIONALLICENCE 700 1- Bierbach Benjamin Clinic for Thoracic- and Cardiovascular Surgery, University of Saarland, Homburg, Germany aut NATIONALLICENCE 700 1- Slotta Jan Institute for Clinical and Experimental Surgery, University of Saarland, Homburg, Germany aut NATIONALLICENCE 700 1- Thorlacius Henrik Department of Surgery, Lund University, Lund, Sweden aut NATIONALLICENCE 700 1- Menger Michael Institute for Clinical and Experimental Surgery, University of Saarland, Homburg, Germany aut NATIONALLICENCE 773 0- Inflammation Research SP Birkhäuser Verlag Basel 60/6(2011-06-01), 555-567 1023-3830 60:6<555 2011 60 11 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer