caa a22 4500 445885106 CHVBK 20180317145556.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s00011-011-0344-3 doi (NATIONALLICENCE)springer-10.1007/s00011-011-0344-3 Neutralization of Neisseria meningitidis outer membrane vesicles [Elektronische Daten] [András Vida, Annet Troelstra, Péter Antal-Szalmás, Toon van Bommel, André Verheul, Jan Verhoef, Kok van Kessel, Jos van Strijp] Introduction: We aimed to determine the neutralization of Neisseria meningitidis outer membrane vesicles (blebs) by humoral and cellular elements of whole blood. Methods: The interaction of FITC-labeled blebs with monocytes was studied by spectrofluorometry. Blebs are able to induce an oxidative burst in neutrophils, and we evaluated the inhibitory effect of plasma on this process. Results: Human plasma reduced the priming activity of blebs containing 1-3ng/ml lipopolysaccharide (LPS) by 50-60% and bactericidal permeability increasing protein (BPI) reduced priming to background levels. A complete neutralization of LPS and blebs by plasma and BPI was measured using the limulus amebocyte lysate (LAL) assay. Furthermore, only 3% of blebs were cell-associated, while the remainder were in the supernatant. Conclusions: Plasma and BPI are able to neutralize blebs, with phagocytosis playing only a minor role. As such, we conclude that blebs do not behave like particles but more like free LPS. Springer Basel AG, 2011 Neisseria meningitidis nationallicence Outer membrane vesicles nationallicence CD14 nationallicence BPI nationallicence LPS nationallicence Vida András Department of Clinical Biochemistry and Molecular Pathology, Medical and Health Science Center, University of Debrecen, Nagyerdei str. 98., 4032, Debrecen, Hungary aut Troelstra Annet Eijkman-Winkler Institute for Microbiology, Infectious Diseases and Inflammation, Utrecht University, Utrecht, The Netherlands aut Antal-Szalmás Péter Department of Clinical Biochemistry and Molecular Pathology, Medical and Health Science Center, University of Debrecen, Nagyerdei str. 98., 4032, Debrecen, Hungary aut van Bommel Toon Eijkman-Winkler Institute for Microbiology, Infectious Diseases and Inflammation, Utrecht University, Utrecht, The Netherlands aut Verheul André Eijkman-Winkler Institute for Microbiology, Infectious Diseases and Inflammation, Utrecht University, Utrecht, The Netherlands aut Verhoef Jan Eijkman-Winkler Institute for Microbiology, Infectious Diseases and Inflammation, Utrecht University, Utrecht, The Netherlands aut van Kessel Kok Eijkman-Winkler Institute for Microbiology, Infectious Diseases and Inflammation, Utrecht University, Utrecht, The Netherlands aut van Strijp Jos Eijkman-Winkler Institute for Microbiology, Infectious Diseases and Inflammation, Utrecht University, Utrecht, The Netherlands aut Inflammation Research SP Birkhäuser Verlag Basel 60/9(2011-09-01), 801-805 1023-3830 60:9<801 2011 60 11 https://doi.org/10.1007/s00011-011-0344-3 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00011-011-0344-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Vida András Department of Clinical Biochemistry and Molecular Pathology, Medical and Health Science Center, University of Debrecen, Nagyerdei str. 98., 4032, Debrecen, Hungary aut NATIONALLICENCE 700 1- Troelstra Annet Eijkman-Winkler Institute for Microbiology, Infectious Diseases and Inflammation, Utrecht University, Utrecht, The Netherlands aut NATIONALLICENCE 700 1- Antal-Szalmás Péter Department of Clinical Biochemistry and Molecular Pathology, Medical and Health Science Center, University of Debrecen, Nagyerdei str. 98., 4032, Debrecen, Hungary aut NATIONALLICENCE 700 1- van Bommel Toon Eijkman-Winkler Institute for Microbiology, Infectious Diseases and Inflammation, Utrecht University, Utrecht, The Netherlands aut NATIONALLICENCE 700 1- Verheul André Eijkman-Winkler Institute for Microbiology, Infectious Diseases and Inflammation, Utrecht University, Utrecht, The Netherlands aut NATIONALLICENCE 700 1- Verhoef Jan Eijkman-Winkler Institute for Microbiology, Infectious Diseases and Inflammation, Utrecht University, Utrecht, The Netherlands aut NATIONALLICENCE 700 1- van Kessel Kok Eijkman-Winkler Institute for Microbiology, Infectious Diseases and Inflammation, Utrecht University, Utrecht, The Netherlands aut NATIONALLICENCE 700 1- van Strijp Jos Eijkman-Winkler Institute for Microbiology, Infectious Diseases and Inflammation, Utrecht University, Utrecht, The Netherlands aut NATIONALLICENCE 773 0- Inflammation Research SP Birkhäuser Verlag Basel 60/9(2011-09-01), 801-805 1023-3830 60:9<801 2011 60 11 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer