caa a22 4500 445885114 CHVBK 20180317145556.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s00011-011-0340-7 doi (NATIONALLICENCE)springer-10.1007/s00011-011-0340-7 GC/MS-based profiling of amino acids and TCA cycle-related molecules in ulcerative colitis [Elektronische Daten] [Makoto Ooi, Shin Nishiumi, Tomoo Yoshie, Yuuki Shiomi, Michitaka Kohashi, Ken Fukunaga, Shiro Nakamura, Takayuki Matsumoto, Naoya Hatano, Masakazu Shinohara, Yasuhiro Irino, Tadaomi Takenawa, Takeshi Azuma, Masaru Yoshida] Objective: The roles that amino acids play in immunity and inflammation are well defined, and the relationship between inflammatory bowel disease (IBD) and certain amino acids has recently attracted attention. In this study, the levels of amino acids and trichloroacetic acid (TCA) cycle-related molecules in the colonic tissues and sera of patients with ulcerative colitis (UC) were profiled by gas chromatography/mass spectrometry (GC/MS), with the aim of evaluating whether the clinical state induced by UC leads to variations in the amino acid profile. Materials and methods: Colonic biopsy samples from 22 UC patients were used, as well as serum samples from UC patients (n=13), Crohn's disease (CD) patients (n=21), and healthy volunteers (n=17). Results: In the GC/MS-based profiling of amino acids and TCA cycle-related molecules, lower levels of 16 amino acids and 5 TCA cycle-related molecules were observed in the colonic lesion tissues of the UC patients, and the serum profiles of amino acids and TCA cycle-related molecules of the UC patients were different from those of the CD patients and healthy volunteers. Conclusions: Our study raises the possibility that GC/MS-based profiling of amino acids and TCA cycle-related molecules is a useful early diagnostic tool for UC. Springer Basel AG, 2011 Ulcerative colitis nationallicence Amino acid nationallicence Metabolomics nationallicence GC/MS nationallicence IBD : Inflammatory bowel disease nationallicence CD : Crohn's disease nationallicence UC : Ulcerative colitis nationallicence ED : Elementary diet nationallicence NMR : Nuclear magnetic resonance analysis nationallicence GC/MS : Gas chromatography/mass spectrometry nationallicence LC/MS : Liquid chromatography/mass spectrometry nationallicence CE/MS : Capillary electrophoresis/mass spectrometry nationallicence TCA : Trichloroacetic acid nationallicence CAI : Clinical activity index nationallicence IOIBD : International Organization for the Study of Inflammatory Bowel Disease nationallicence MSTFA : N-Methyl-N-trimethylsilyl-trifluoroacetamide nationallicence PCA : Principal component analysis nationallicence PLS-DA : Partial least squares discriminant analysis nationallicence Ooi Makoto Division of Gastroenterology, Department of Internal Medicine, Kobe University Graduate School of Medicine, 7-5-1, Chuo-ku, Kusunoki-cho, 650-0017, Kobe, Hyogo, Japan aut Nishiumi Shin Division of Gastroenterology, Department of Internal Medicine, Kobe University Graduate School of Medicine, 7-5-1, Chuo-ku, Kusunoki-cho, 650-0017, Kobe, Hyogo, Japan aut Yoshie Tomoo Division of Gastroenterology, Department of Internal Medicine, Kobe University Graduate School of Medicine, 7-5-1, Chuo-ku, Kusunoki-cho, 650-0017, Kobe, Hyogo, Japan aut Shiomi Yuuki Division of Gastroenterology, Department of Internal Medicine, Kobe University Graduate School of Medicine, 7-5-1, Chuo-ku, Kusunoki-cho, 650-0017, Kobe, Hyogo, Japan aut Kohashi Michitaka Division of Gastroenterology, Department of Internal Medicine, Kobe University Graduate School of Medicine, 7-5-1, Chuo-ku, Kusunoki-cho, 650-0017, Kobe, Hyogo, Japan aut Fukunaga Ken The Divisions of Lower Gastroenterology, Department of Internal Medicine, Hyogo College of Medicine, Nishinomiya, Japan aut Nakamura Shiro The Divisions of Lower Gastroenterology, Department of Internal Medicine, Hyogo College of Medicine, Nishinomiya, Japan aut Matsumoto Takayuki The Divisions of Lower Gastroenterology, Department of Internal Medicine, Hyogo College of Medicine, Nishinomiya, Japan aut Hatano Naoya The Integrated Center for Mass Spectrometry, Kobe University Graduate School of Medicine, Kobe, Japan aut Shinohara Masakazu The Integrated Center for Mass Spectrometry, Kobe University Graduate School of Medicine, Kobe, Japan aut Irino Yasuhiro The Integrated Center for Mass Spectrometry, Kobe University Graduate School of Medicine, Kobe, Japan aut Takenawa Tadaomi The Integrated Center for Mass Spectrometry, Kobe University Graduate School of Medicine, Kobe, Japan aut Azuma Takeshi Division of Gastroenterology, Department of Internal Medicine, Kobe University Graduate School of Medicine, 7-5-1, Chuo-ku, Kusunoki-cho, 650-0017, Kobe, Hyogo, Japan aut Yoshida Masaru Division of Gastroenterology, Department of Internal Medicine, Kobe University Graduate School of Medicine, 7-5-1, Chuo-ku, Kusunoki-cho, 650-0017, Kobe, Hyogo, Japan aut Inflammation Research SP Birkhäuser Verlag Basel 60/9(2011-09-01), 831-840 1023-3830 60:9<831 2011 60 11 https://doi.org/10.1007/s00011-011-0340-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00011-011-0340-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ooi Makoto Division of Gastroenterology, Department of Internal Medicine, Kobe University Graduate School of Medicine, 7-5-1, Chuo-ku, Kusunoki-cho, 650-0017, Kobe, Hyogo, Japan aut NATIONALLICENCE 700 1- Nishiumi Shin Division of Gastroenterology, Department of Internal Medicine, Kobe University Graduate School of Medicine, 7-5-1, Chuo-ku, Kusunoki-cho, 650-0017, Kobe, Hyogo, Japan aut NATIONALLICENCE 700 1- Yoshie Tomoo Division of Gastroenterology, Department of Internal Medicine, Kobe University Graduate School of Medicine, 7-5-1, Chuo-ku, Kusunoki-cho, 650-0017, Kobe, Hyogo, Japan aut NATIONALLICENCE 700 1- Shiomi Yuuki Division of Gastroenterology, Department of Internal Medicine, Kobe University Graduate School of Medicine, 7-5-1, Chuo-ku, Kusunoki-cho, 650-0017, Kobe, Hyogo, Japan aut NATIONALLICENCE 700 1- Kohashi Michitaka Division of Gastroenterology, Department of Internal Medicine, Kobe University Graduate School of Medicine, 7-5-1, Chuo-ku, Kusunoki-cho, 650-0017, Kobe, Hyogo, Japan aut NATIONALLICENCE 700 1- Fukunaga Ken The Divisions of Lower Gastroenterology, Department of Internal Medicine, Hyogo College of Medicine, Nishinomiya, Japan aut NATIONALLICENCE 700 1- Nakamura Shiro The Divisions of Lower Gastroenterology, Department of Internal Medicine, Hyogo College of Medicine, Nishinomiya, Japan aut NATIONALLICENCE 700 1- Matsumoto Takayuki The Divisions of Lower Gastroenterology, Department of Internal Medicine, Hyogo College of Medicine, Nishinomiya, Japan aut NATIONALLICENCE 700 1- Hatano Naoya The Integrated Center for Mass Spectrometry, Kobe University Graduate School of Medicine, Kobe, Japan aut NATIONALLICENCE 700 1- Shinohara Masakazu The Integrated Center for Mass Spectrometry, Kobe University Graduate School of Medicine, Kobe, Japan aut NATIONALLICENCE 700 1- Irino Yasuhiro The Integrated Center for Mass Spectrometry, Kobe University Graduate School of Medicine, Kobe, Japan aut NATIONALLICENCE 700 1- Takenawa Tadaomi The Integrated Center for Mass Spectrometry, Kobe University Graduate School of Medicine, Kobe, Japan aut NATIONALLICENCE 700 1- Azuma Takeshi Division of Gastroenterology, Department of Internal Medicine, Kobe University Graduate School of Medicine, 7-5-1, Chuo-ku, Kusunoki-cho, 650-0017, Kobe, Hyogo, Japan aut NATIONALLICENCE 700 1- Yoshida Masaru Division of Gastroenterology, Department of Internal Medicine, Kobe University Graduate School of Medicine, 7-5-1, Chuo-ku, Kusunoki-cho, 650-0017, Kobe, Hyogo, Japan aut NATIONALLICENCE 773 0- Inflammation Research SP Birkhäuser Verlag Basel 60/9(2011-09-01), 831-840 1023-3830 60:9<831 2011 60 11 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer