caa a22 4500 445885122 CHVBK 20180317145556.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s00011-011-0346-1 doi (NATIONALLICENCE)springer-10.1007/s00011-011-0346-1 Role of heme oxygenase-1 in inflammatory response induced by mechanical stretch in synovial cells [Elektronische Daten] [Masanobu Takao, Toshinori Okinaga, Wataru Ariyoshi, Kenjiro Iwanaga, Ikuo Nakamichi, Izumi Yoshioka, Kazuhiro Tominaga, Tatsuji Nishihara] Objective: The purpose of this study was to investigate the mechanism by which heme oxygenase-1 (HO-1) regulates inflammatory responses induced by mechanical stretch in human fibroblast-like synoviocyte (HFLS) cells. Materials and methods: HFLS cells were cultured in the presence of hemin and seeded into fibronectin-coated silicon chambers. The chambers were attached to a stretching apparatus which applied a uniaxial sinusoidal stretching force. The genetic expressions of cyclooxygenase-2 (COX-2), interleukin-1β (IL-1β) and HO-1 were analyzed using real-time RT-PCR. The expression and localization of HO-1 protein were detected by immunofluorescence staining. The amounts of prostaglandin E2 (PGE2) released into the culture medium were determined using ELISA. Results: Mechanical stretch enhanced the expressions of COX-2 and IL-1β, and the amount of PGE2 synthesis in HFLS cells, whereas that of HO-1 was slightly increased. In contrast, treatment with hemin enhanced HO-1 gene expression and mechanical stretch enhanced this expression in hemin-pretreated cells. In addition, hemin pretreatment suppressed PGE2 synthesis induced by mechanical stretch. Conclusion: We found that constitutive HO-1 expression in hemin-pretreated HFLS cells suppressed mechanical stretch-induced inflammatory responses, suggesting that HO-1 may play a role as a regulation factor in synovial tissue inflammation. Springer Basel AG, 2011 Mechanical stretch nationallicence COX-2 nationallicence IL-1β nationallicence HO-1 nationallicence PGE2 nationallicence Synovial cell nationallicence Takao Masanobu Division of Infection and Molecular Biology, Department of Health Promotion, Kyushu Dental College, 2-6-1 Manazuru Kokurakita-ku, 803-8580, Kitakyushu, Fukuoka, Japan aut Okinaga Toshinori Division of Infection and Molecular Biology, Department of Health Promotion, Kyushu Dental College, 2-6-1 Manazuru Kokurakita-ku, 803-8580, Kitakyushu, Fukuoka, Japan aut Ariyoshi Wataru Division of Infection and Molecular Biology, Department of Health Promotion, Kyushu Dental College, 2-6-1 Manazuru Kokurakita-ku, 803-8580, Kitakyushu, Fukuoka, Japan aut Iwanaga Kenjiro Division of Maxillofacial Diagnostic and Surgical Science, Department of Oral and Maxillofacial Surgery, Kyushu Dental College, 803-8580, Kitakyushu, Japan aut Nakamichi Ikuo Division of General Internal Medicine, Department of Health Promotion, Kyushu Dental College, 803-8580, Kitakyushu, Japan aut Yoshioka Izumi Department of Oral and Maxillofacial Surgery, Faculty of Medicine, University of Miyazaki, 889-1692, Kiyotake Miyazaki, Japan aut Tominaga Kazuhiro Division of Maxillofacial Diagnostic and Surgical Science, Department of Oral and Maxillofacial Surgery, Kyushu Dental College, 803-8580, Kitakyushu, Japan aut Nishihara Tatsuji Division of Infection and Molecular Biology, Department of Health Promotion, Kyushu Dental College, 2-6-1 Manazuru Kokurakita-ku, 803-8580, Kitakyushu, Fukuoka, Japan aut Inflammation Research SP Birkhäuser Verlag Basel 60/9(2011-09-01), 861-867 1023-3830 60:9<861 2011 60 11 https://doi.org/10.1007/s00011-011-0346-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00011-011-0346-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Takao Masanobu Division of Infection and Molecular Biology, Department of Health Promotion, Kyushu Dental College, 2-6-1 Manazuru Kokurakita-ku, 803-8580, Kitakyushu, Fukuoka, Japan aut NATIONALLICENCE 700 1- Okinaga Toshinori Division of Infection and Molecular Biology, Department of Health Promotion, Kyushu Dental College, 2-6-1 Manazuru Kokurakita-ku, 803-8580, Kitakyushu, Fukuoka, Japan aut NATIONALLICENCE 700 1- Ariyoshi Wataru Division of Infection and Molecular Biology, Department of Health Promotion, Kyushu Dental College, 2-6-1 Manazuru Kokurakita-ku, 803-8580, Kitakyushu, Fukuoka, Japan aut NATIONALLICENCE 700 1- Iwanaga Kenjiro Division of Maxillofacial Diagnostic and Surgical Science, Department of Oral and Maxillofacial Surgery, Kyushu Dental College, 803-8580, Kitakyushu, Japan aut NATIONALLICENCE 700 1- Nakamichi Ikuo Division of General Internal Medicine, Department of Health Promotion, Kyushu Dental College, 803-8580, Kitakyushu, Japan aut NATIONALLICENCE 700 1- Yoshioka Izumi Department of Oral and Maxillofacial Surgery, Faculty of Medicine, University of Miyazaki, 889-1692, Kiyotake Miyazaki, Japan aut NATIONALLICENCE 700 1- Tominaga Kazuhiro Division of Maxillofacial Diagnostic and Surgical Science, Department of Oral and Maxillofacial Surgery, Kyushu Dental College, 803-8580, Kitakyushu, Japan aut NATIONALLICENCE 700 1- Nishihara Tatsuji Division of Infection and Molecular Biology, Department of Health Promotion, Kyushu Dental College, 2-6-1 Manazuru Kokurakita-ku, 803-8580, Kitakyushu, Fukuoka, Japan aut NATIONALLICENCE 773 0- Inflammation Research SP Birkhäuser Verlag Basel 60/9(2011-09-01), 861-867 1023-3830 60:9<861 2011 60 11 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer