caa a22 4500 445885130 CHVBK 20180317145557.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s00011-011-0345-2 doi (NATIONALLICENCE)springer-10.1007/s00011-011-0345-2 Methyl-1-hydroxy-2-naphthoate, a novel naphthol derivative, inhibits lipopolysaccharide-induced inflammatory response in macrophages via suppression of NF-κB, JNK and p38 MAPK pathways [Elektronische Daten] [Jun-Yan Zhang, Hong Jin, Guang-Fa Wang, Peng-Jiu Yu, Shao-Yu Wu, Zheng-Guang Zhu, Zhong-Huang Li, Yuan-Xin Tian, Wei Xu, Jia-Jie Zhang, Shu-Guang Wu] Objective and design: The anti-inflammatory effect of methyl-1-hydroxy-2-naphthoate (MHNA), a novel naphthol derivative, was evaluated in the lipopolysaccharide (LPS)-induced inflammatory response in murine macrophages. Materials and methods: The release of nitric oxide (NO), interleukin-1beta (IL-1β) and interleukin-6 (IL-6) were detected by the Griess reagent and ELISA methods. The protein expressions of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2) were examined by Western blotting. The mRNA expressions of IL-1β, IL-6, iNOS and COX-2 were determined by real-time PCR. Activation of mitogen-activated protein kinases (MAPKs) and nuclear factor kappa B (NF-κB) pathways were detected by Western blotting, reporter gene assay and electrophoretic mobility shift assay. Results: MHNA significantly inhibited the release of NO, IL-1β and IL-6 as well as the protein expression of iNOS and COX-2 in LPS-stimulated macrophages. It also inhibited the mRNA expression of iNOS, COX-2, IL-1β and IL-6. Further studies indicated that MHNA inhibited LPS-induced increases in NF-κB DNA-binding activity and NF-κB transcriptional activity as well as IκB-α degradation and NF-κB translocation in a dose-dependent manner. Meanwhile, the activation of p38 MAPK and c-Jun N-terminal kinases (JNK) induced by LPS were decreased by MHNA. Conclusions: MHNA inhibits the LPS-induced inflammatory response in murine macrophages via suppression of NF-κB and MAPKs signaling pathways activation. Springer Basel AG, 2011 Methyl-1-hydroxy-2-naphthoate nationallicence Naphthol nationallicence NF-κB nationallicence Macrophage nationallicence Inflammatory mediators nationallicence COX-2 : Cyclooxygenase-2 nationallicence EMSA : Electrophoretic mobility shift assay nationallicence ERK : Extracellular signal-regulated kinases nationallicence iNOS : Inducible nitric oxide synthase nationallicence IκB : Inhibitory kappa B nationallicence IL-1β : Interleukin-1beta nationallicence IL-6 : Interleukin-6 nationallicence JNK : c-Jun N-terminal kinases nationallicence LPS : Lipopolysaccharide nationallicence MAPKs : Mitogen-activated protein kinases nationallicence NO : Nitric oxide nationallicence NF-κB : Nuclear factor kappa B nationallicence PGE2 : Prostaglandin E2 nationallicence TNF-α : Tumor necrosis factor alpha nationallicence Zhang Jun-Yan School of Pharmaceutical Sciences, Southern Medical University, 510515, Guangzhou, China aut Jin Hong School of Pharmaceutical Sciences, Southern Medical University, 510515, Guangzhou, China aut Wang Guang-Fa School of Pharmaceutical Sciences, Southern Medical University, 510515, Guangzhou, China aut Yu Peng-Jiu School of Pharmaceutical Sciences, Southern Medical University, 510515, Guangzhou, China aut Wu Shao-Yu School of Pharmaceutical Sciences, Southern Medical University, 510515, Guangzhou, China aut Zhu Zheng-Guang School of Pharmaceutical Sciences, Southern Medical University, 510515, Guangzhou, China aut Li Zhong-Huang School of Pharmaceutical Sciences, Southern Medical University, 510515, Guangzhou, China aut Tian Yuan-Xin School of Pharmaceutical Sciences, Southern Medical University, 510515, Guangzhou, China aut Xu Wei School of Pharmaceutical Sciences, Southern Medical University, 510515, Guangzhou, China aut Zhang Jia-Jie School of Pharmaceutical Sciences, Southern Medical University, 510515, Guangzhou, China aut Wu Shu-Guang School of Pharmaceutical Sciences, Southern Medical University, 510515, Guangzhou, China aut Inflammation Research SP Birkhäuser Verlag Basel 60/9(2011-09-01), 851-859 1023-3830 60:9<851 2011 60 11 https://doi.org/10.1007/s00011-011-0345-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00011-011-0345-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Jun-Yan School of Pharmaceutical Sciences, Southern Medical University, 510515, Guangzhou, China aut NATIONALLICENCE 700 1- Jin Hong School of Pharmaceutical Sciences, Southern Medical University, 510515, Guangzhou, China aut NATIONALLICENCE 700 1- Wang Guang-Fa School of Pharmaceutical Sciences, Southern Medical University, 510515, Guangzhou, China aut NATIONALLICENCE 700 1- Yu Peng-Jiu School of Pharmaceutical Sciences, Southern Medical University, 510515, Guangzhou, China aut NATIONALLICENCE 700 1- Wu Shao-Yu School of Pharmaceutical Sciences, Southern Medical University, 510515, Guangzhou, China aut NATIONALLICENCE 700 1- Zhu Zheng-Guang School of Pharmaceutical Sciences, Southern Medical University, 510515, Guangzhou, China aut NATIONALLICENCE 700 1- Li Zhong-Huang School of Pharmaceutical Sciences, Southern Medical University, 510515, Guangzhou, China aut NATIONALLICENCE 700 1- Tian Yuan-Xin School of Pharmaceutical Sciences, Southern Medical University, 510515, Guangzhou, China aut NATIONALLICENCE 700 1- Xu Wei School of Pharmaceutical Sciences, Southern Medical University, 510515, Guangzhou, China aut NATIONALLICENCE 700 1- Zhang Jia-Jie School of Pharmaceutical Sciences, Southern Medical University, 510515, Guangzhou, China aut NATIONALLICENCE 700 1- Wu Shu-Guang School of Pharmaceutical Sciences, Southern Medical University, 510515, Guangzhou, China aut NATIONALLICENCE 773 0- Inflammation Research SP Birkhäuser Verlag Basel 60/9(2011-09-01), 851-859 1023-3830 60:9<851 2011 60 11 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer