caa a22 4500 445885157 CHVBK 20180317145557.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s00011-011-0337-2 doi (NATIONALLICENCE)springer-10.1007/s00011-011-0337-2 Folic acid inhibits lipopolysaccharide-induced inflammatory response in RAW264.7 macrophages by suppressing MAPKs and NF-κB activation [Elektronische Daten] [Dan Feng, Yan Zhou, Min Xia, Jing Ma] Objective: The aim of the present study was to investigate the effects of folic acid on the inflammatory responses to lipopolysaccharide (LPS) in RAW264.7 cells and the signal transduction pathways involved. Methods: RAW264.7 macrophages were used. The production of nitric oxide (NO) was determined by the Griess test. The protein levels and mRNA expression of tumor necrosis factor-alpha (TNF-α) and interleukin-1 beta (IL-1β) were determined by ELISA and RT-PCR, respectively. The phosphorylation of mitogen-activated protein kinases (MAPKs) and nuclear nuclear factor-kappa B (NF-κB) p65 protein were analyzed by Western blotting. Results: Folic acid inhibited LPS-induced production of NO, TNF-α and IL-1β with decreased mRNAs of inducible nitric oxide synthase (iNOS), TNF-α and IL-1β. Further study showed that folic acid inhibited the LPS-induced phosphorylation of MAPKs and the nuclear translocation of NF-κB. Conclusion: Folic acid inhibits the inflammatory response of RAW 264.7 cells to LPS through inhibiting the MAPKs and NF-κB pathways. Springer Basel AG, 2011 Folic acid nationallicence Mitogen-activated protein kinases nationallicence Nitric oxide nationallicence Tumor necrosis factor-alpha nationallicence Interleukin-1 beta nationallicence Feng Dan Department of Preventive Medicine, School of Public Health, Sun Yat-sen University, 74 Zhongshan Road 2, 510080, Guangzhou, People's Republic of China aut Zhou Yan Guangdong Provincial Key Laboratory of Food, Nutrition and Health, School of Public Health, Sun Yat-sen University (Northern Campus), 74 Zhongshan Road 2, 510080, Guangzhou, People's Republic of China aut Xia Min Guangdong Provincial Key Laboratory of Food, Nutrition and Health, School of Public Health, Sun Yat-sen University (Northern Campus), 74 Zhongshan Road 2, 510080, Guangzhou, People's Republic of China aut Ma Jing Guangdong Provincial Key Laboratory of Food, Nutrition and Health, School of Public Health, Sun Yat-sen University (Northern Campus), 74 Zhongshan Road 2, 510080, Guangzhou, People's Republic of China aut Inflammation Research SP Birkhäuser Verlag Basel 60/9(2011-09-01), 817-822 1023-3830 60:9<817 2011 60 11 https://doi.org/10.1007/s00011-011-0337-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00011-011-0337-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Feng Dan Department of Preventive Medicine, School of Public Health, Sun Yat-sen University, 74 Zhongshan Road 2, 510080, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Zhou Yan Guangdong Provincial Key Laboratory of Food, Nutrition and Health, School of Public Health, Sun Yat-sen University (Northern Campus), 74 Zhongshan Road 2, 510080, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Xia Min Guangdong Provincial Key Laboratory of Food, Nutrition and Health, School of Public Health, Sun Yat-sen University (Northern Campus), 74 Zhongshan Road 2, 510080, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Ma Jing Guangdong Provincial Key Laboratory of Food, Nutrition and Health, School of Public Health, Sun Yat-sen University (Northern Campus), 74 Zhongshan Road 2, 510080, Guangzhou, People's Republic of China aut NATIONALLICENCE 773 0- Inflammation Research SP Birkhäuser Verlag Basel 60/9(2011-09-01), 817-822 1023-3830 60:9<817 2011 60 11 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer