caa a22 4500 445885165 CHVBK 20180317145557.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s00011-011-0342-5 doi (NATIONALLICENCE)springer-10.1007/s00011-011-0342-5 LPS pretreatment ameliorates multiple organ injuries and improves survival in a murine model of polymicrobial sepsis [Elektronische Daten] [Dong-Wei Shi, Jing Zhang, Hong-Ni Jiang, Chao-Yang Tong, Guo-Rong Gu, Yuan Ji, Hanssa Summah, Jie-Ming Qu] Objective: The endotoxin tolerance phenotype is characterized with decreased inflammation and increased phagocytosis. We hypothesized that endotoxin tolerance would provide protective effects on experimental sepsis with multiple organ injuries induced by cecal ligation and puncture (CLP). Methods: Endotoxin tolerance was induced in male Sprague-Dawley rats with daily intraperitoneal injection of either 0.6mg/kg of lipopolysaccharide (LPS) or vehicle for four consecutive days before subsequent CLP. Biochemical parameters, histological changes, inflammatory cytokine production, and lung tissue nuclear factor-κB (NF-κB) activation were assessed post-CLP. In a separate experiment, survival rate was monitored for 7days after CLP. Results: In vehicle-treated animals, CLP caused multiple organ injuries confirmed by the biochemical variables and histological examination. This was accompanied by an early activation of NF-κB in the lung and a substantial increase in plasma levels of tumor necrosis factor-α, interleukin-6, and interleukin-10. In contrast, pretreatment with LPS not only alleviated the development of multiple organ injuries after CLP, but also decreased sepsis-induced activation of pulmonary NF-κB and reduced plasma cytokines production. In addition, LPS pretreatment improved the survival in rats subjected to CLP. Conclusions: The beneficial effects of endotoxin tolerance indicate the potential of immunomodulatory strategies in the management of severe sepsis. Springer Basel AG, 2011 Endotoxin tolerance nationallicence Cecal ligation and puncture nationallicence Sepsis nationallicence Multiple organ dysfunction syndrome nationallicence Cytokines nationallicence Shi Dong-Wei Department of Emergency Medicine, Zhongshan Hospital, Shanghai Medical College, Fudan University, Shanghai, China aut Zhang Jing Department of Pulmonary Medicine, Zhongshan Hospital, Shanghai Medical College, Fudan University, Shanghai, China aut Jiang Hong-Ni Department of Pulmonary Medicine, Zhongshan Hospital, Shanghai Medical College, Fudan University, Shanghai, China aut Tong Chao-Yang Department of Emergency Medicine, Zhongshan Hospital, Shanghai Medical College, Fudan University, Shanghai, China aut Gu Guo-Rong Department of Emergency Medicine, Zhongshan Hospital, Shanghai Medical College, Fudan University, Shanghai, China aut Ji Yuan Department of Pathology, Zhongshan Hospital, Shanghai Medical College, Fudan University, Shanghai, China aut Summah Hanssa Department of Pulmonary Medicine, Huadong Hospital, Shanghai Medical College, Fudan University, 200040, Shanghai, China aut Qu Jie-Ming Department of Pulmonary Medicine, Huadong Hospital, Shanghai Medical College, Fudan University, 200040, Shanghai, China aut Inflammation Research SP Birkhäuser Verlag Basel 60/9(2011-09-01), 841-849 1023-3830 60:9<841 2011 60 11 https://doi.org/10.1007/s00011-011-0342-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00011-011-0342-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shi Dong-Wei Department of Emergency Medicine, Zhongshan Hospital, Shanghai Medical College, Fudan University, Shanghai, China aut NATIONALLICENCE 700 1- Zhang Jing Department of Pulmonary Medicine, Zhongshan Hospital, Shanghai Medical College, Fudan University, Shanghai, China aut NATIONALLICENCE 700 1- Jiang Hong-Ni Department of Pulmonary Medicine, Zhongshan Hospital, Shanghai Medical College, Fudan University, Shanghai, China aut NATIONALLICENCE 700 1- Tong Chao-Yang Department of Emergency Medicine, Zhongshan Hospital, Shanghai Medical College, Fudan University, Shanghai, China aut NATIONALLICENCE 700 1- Gu Guo-Rong Department of Emergency Medicine, Zhongshan Hospital, Shanghai Medical College, Fudan University, Shanghai, China aut NATIONALLICENCE 700 1- Ji Yuan Department of Pathology, Zhongshan Hospital, Shanghai Medical College, Fudan University, Shanghai, China aut NATIONALLICENCE 700 1- Summah Hanssa Department of Pulmonary Medicine, Huadong Hospital, Shanghai Medical College, Fudan University, 200040, Shanghai, China aut NATIONALLICENCE 700 1- Qu Jie-Ming Department of Pulmonary Medicine, Huadong Hospital, Shanghai Medical College, Fudan University, 200040, Shanghai, China aut NATIONALLICENCE 773 0- Inflammation Research SP Birkhäuser Verlag Basel 60/9(2011-09-01), 841-849 1023-3830 60:9<841 2011 60 11 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer