caa a22 4500 445885181 CHVBK 20180317145557.0 cr unu---uuuuu 170323e20110701xx s 000 0 eng 10.1007/s00011-011-0321-x doi (NATIONALLICENCE)springer-10.1007/s00011-011-0321-x Anti-inflammatory effect of α,β-amyrin, a triterpene from Protium heptaphyllum , on cerulein-induced acute pancreatitis in mice [Elektronische Daten] [Caroline Melo, Talita Morais, Adriana Tomé, Gerly Brito, Mariana Chaves, Vietla Rao, Flávia Santos] Objective: To evaluate the anti-inflammatory effect of α,β-amyrin, a pentacyclic triterpenoid from Protium heptaphyllum, on cerulein-induced acute pancreatitis in mice. Methods: Acute pancreatitis was induced in Swiss mice by five intraperitoneal injections of cerulein (50μg/kg), at 1h intervals. Mice received α,β-amyrin (10, 30 and 100mg/kg), thalidomide (200mg/kg), or vehicle (3% Tween 80) orally 1h before and 12h after the cerulein challenge. The severity of pancreatitis was evaluated 24h after cerulein by assessing serum pro-inflammatory cytokines and amylase activity, pancreatic myeloperoxidase (MPO), and thiobarbituric acid-reactive substances (TBARS), as well as by histology. Results: α,β-Amyrin and thalidomide significantly attenuated the cerulein-induced increase in tumor necrosis factor (TNF)-α, interleukin-6, lipase, amylase, MPO, and TBARS. Moreover, α,β-amyrin greatly suppressed the pancreatic edema, inflammatory cell infiltration, acinar cell necrosis, and expressions of TNFα and inducible nitric oxide synthase. Conclusions: α,β-Amyrin ameliorates cerulein-induced acute pancreatitis by acting as an anti-inflammatory and antioxidant agent. Springer Basel AG, 2011 Acute pancreatitis nationallicence Amyrin nationallicence Cerulein nationallicence Pro-inflammatory cytokines nationallicence Mice nationallicence Melo Caroline Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceará, Cel Nunes de Melo, 1127, Rodolfo Teófilo, 60430-270, Fortaleza, CE, Brazil aut Morais Talita Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceará, Cel Nunes de Melo, 1127, Rodolfo Teófilo, 60430-270, Fortaleza, CE, Brazil aut Tomé Adriana Faculty of Veterinary Medicine, State University of Ceará, Paranjana, 1700, Itaperi, 60740-000, Fortaleza, CE, Brazil aut Brito Gerly National Institute of Biomedicine of the Brazilian SemiArid, Faculty of Medicine, Federal University of Ceará, Cel Nunes de Melo, 1315, Rodolfo Teófilo, 60430-270, Fortaleza, CE, Brazil aut Chaves Mariana Department of Chemistry, Federal University of Piauí Campus Ministro Petrônio Portela, Ininga, 64049-550, Teresina, PI, Brazil aut Rao Vietla Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceará, Cel Nunes de Melo, 1127, Rodolfo Teófilo, 60430-270, Fortaleza, CE, Brazil aut Santos Flávia Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceará, Cel Nunes de Melo, 1127, Rodolfo Teófilo, 60430-270, Fortaleza, CE, Brazil aut Inflammation Research SP Birkhäuser Verlag Basel 60/7(2011-07-01), 673-681 1023-3830 60:7<673 2011 60 11 https://doi.org/10.1007/s00011-011-0321-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00011-011-0321-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Melo Caroline Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceará, Cel Nunes de Melo, 1127, Rodolfo Teófilo, 60430-270, Fortaleza, CE, Brazil aut NATIONALLICENCE 700 1- Morais Talita Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceará, Cel Nunes de Melo, 1127, Rodolfo Teófilo, 60430-270, Fortaleza, CE, Brazil aut NATIONALLICENCE 700 1- Tomé Adriana Faculty of Veterinary Medicine, State University of Ceará, Paranjana, 1700, Itaperi, 60740-000, Fortaleza, CE, Brazil aut NATIONALLICENCE 700 1- Brito Gerly National Institute of Biomedicine of the Brazilian SemiArid, Faculty of Medicine, Federal University of Ceará, Cel Nunes de Melo, 1315, Rodolfo Teófilo, 60430-270, Fortaleza, CE, Brazil aut NATIONALLICENCE 700 1- Chaves Mariana Department of Chemistry, Federal University of Piauí Campus Ministro Petrônio Portela, Ininga, 64049-550, Teresina, PI, Brazil aut NATIONALLICENCE 700 1- Rao Vietla Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceará, Cel Nunes de Melo, 1127, Rodolfo Teófilo, 60430-270, Fortaleza, CE, Brazil aut NATIONALLICENCE 700 1- Santos Flávia Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceará, Cel Nunes de Melo, 1127, Rodolfo Teófilo, 60430-270, Fortaleza, CE, Brazil aut NATIONALLICENCE 773 0- Inflammation Research SP Birkhäuser Verlag Basel 60/7(2011-07-01), 673-681 1023-3830 60:7<673 2011 60 11 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer