caa a22 4500 445885238 CHVBK 20180317145557.0 cr unu---uuuuu 170323e20110701xx s 000 0 eng 10.1007/s00011-011-0323-8 doi (NATIONALLICENCE)springer-10.1007/s00011-011-0323-8 Anti-inflammatory properties of N -acetylcysteine on lipopolysaccharide-activated macrophages [Elektronische Daten] [J. Palacio, U. Markert, P. Martínez] Introduction: Innate immune cells play a role in modulating host immune response. Part of the macrophage inflammatory response is the release of an array of inflammatory cytokines, important molecules during the development of innate and adaptative immunity. Several antioxidant agents have been used in the control of the inflammatory response. Objective: To evaluate the anti-inflammatory effect of N-acetylcysteine (NAC) on the expression and secretion of inflammatory cytokines and interleukin (IL)-10 in lipopolysaccharide (LPS)-activated THP-1 macrophages under mild oxidative conditions. Methods: Macrophages were activated by LPS (0.1 and 1μg/ml) for up to 24h. The effect of 15mM NAC was evaluated at 2, 4, 6 and 24h. mRNA expression of tumor necrosis factor (TNF)-α, IL-1β, IL-6, IL-8 and IL-10 was assessed by real time PCR. The expression of the corresponding cytokines plus IL-12p70 was analyzed using a bead array for flow cytometry. Results: NAC inhibits the inflammatory cytokines TNFα, IL-1β and IL-6 in LPS-activated macrophages under mild oxidative conditions. IL-10 mRNA and protein expression are strongly downregulated in NAC-treated cells, which may further modify the inflammatory cytokine profile. Conclusion: NAC modulates immune functions during the inflammatory response. Springer Basel AG, 2011 Inflammatory cytokines nationallicence N -Acetylcysteine (NAC) nationallicence Reactive oxygen species (ROS) nationallicence THP-1 induced-macrophage nationallicence Palacio J. Instituto de Biotecnología y Biomedicina, Universidad Autónoma de Barcelona, 08193, Bellaterra, Barcelona, Spain aut Markert U. Klinik für Frauenheilkunde und Geburtshilfe, Abteilung für Geburtshilfe, Placenta-Labor, Friedrich Schiller-Universität Jena, Jena, Germany aut Martínez P. Instituto de Biotecnología y Biomedicina, Universidad Autónoma de Barcelona, 08193, Bellaterra, Barcelona, Spain aut Inflammation Research SP Birkhäuser Verlag Basel 60/7(2011-07-01), 695-704 1023-3830 60:7<695 2011 60 11 https://doi.org/10.1007/s00011-011-0323-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00011-011-0323-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Palacio J. Instituto de Biotecnología y Biomedicina, Universidad Autónoma de Barcelona, 08193, Bellaterra, Barcelona, Spain aut NATIONALLICENCE 700 1- Markert U. Klinik für Frauenheilkunde und Geburtshilfe, Abteilung für Geburtshilfe, Placenta-Labor, Friedrich Schiller-Universität Jena, Jena, Germany aut NATIONALLICENCE 700 1- Martínez P. Instituto de Biotecnología y Biomedicina, Universidad Autónoma de Barcelona, 08193, Bellaterra, Barcelona, Spain aut NATIONALLICENCE 773 0- Inflammation Research SP Birkhäuser Verlag Basel 60/7(2011-07-01), 695-704 1023-3830 60:7<695 2011 60 11 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer