caa a22 4500 445885270 CHVBK 20180317145557.0 cr unu---uuuuu 170323e20110701xx s 000 0 eng 10.1007/s00011-011-0324-7 doi (NATIONALLICENCE)springer-10.1007/s00011-011-0324-7 Dectin-1 and NOD2 mediate cathepsin activation in zymosan-induced arthritis in mice [Elektronische Daten] [Holly Rosenzweig, Jenna Clowers, Gabriel Nunez, James Rosenbaum, Michael Davey] Objective: Activation of pattern recognition receptors (PRR) may contribute to arthritis. Here, we elucidated the role of NOD2, a genetic cause of inflammatory arthritis, and several other PRR in a murine model of inflammatory arthritis. Methods: The roles of CR3, TLR2, MyD88, NOD1, NOD2, Dectin-1 and Dectin-2 were tested in vivo in arthritis elicited by intra-articular injections of zymosan, the fungal cell wall components curdlan, laminarin and mannan, and the bacterial cell wall peptidoglycan. Results: Dectin-1, and to a lesser extent Dectin-2, contributed to arthritis. TLR2, MyD88 and CR3 played non-essential roles. Observations based on injection of curdlan, laminarin or mannan supported the dominant role of the Dectin-1 pathway in the joint. We demonstrated differential roles for NOD1 and NOD2 and identified NOD2 as a novel and essential mediator of zymosan-induced arthritis. Conclusions: Together, Dectin-1 and NOD2 are critical, sentinel receptors in the arthritogenic effects of zymosan. Our data identify a novel role for NOD2 during inflammatory responses within joints. Springer Basel AG, 2011 Arthritis models nationallicence NOD2 nationallicence In vivo inflammation nationallicence Innate immunity nationallicence Zymosan nationallicence Rosenzweig Holly Oregon Health and Science University, Mail stop: L467 AD, 3181 SW Sam Jackson Park Rd, 97239, Portland, OR, USA aut Clowers Jenna Oregon Health and Science University, Mail stop: L467 AD, 3181 SW Sam Jackson Park Rd, 97239, Portland, OR, USA aut Nunez Gabriel University of Michigan Medical School, Ann Arbor, MI, USA aut Rosenbaum James Oregon Health and Science University, Mail stop: L467 AD, 3181 SW Sam Jackson Park Rd, 97239, Portland, OR, USA aut Davey Michael Oregon Health and Science University, Mail stop: L467 AD, 3181 SW Sam Jackson Park Rd, 97239, Portland, OR, USA aut Inflammation Research SP Birkhäuser Verlag Basel 60/7(2011-07-01), 705-714 1023-3830 60:7<705 2011 60 11 https://doi.org/10.1007/s00011-011-0324-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00011-011-0324-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rosenzweig Holly Oregon Health and Science University, Mail stop: L467 AD, 3181 SW Sam Jackson Park Rd, 97239, Portland, OR, USA aut NATIONALLICENCE 700 1- Clowers Jenna Oregon Health and Science University, Mail stop: L467 AD, 3181 SW Sam Jackson Park Rd, 97239, Portland, OR, USA aut NATIONALLICENCE 700 1- Nunez Gabriel University of Michigan Medical School, Ann Arbor, MI, USA aut NATIONALLICENCE 700 1- Rosenbaum James Oregon Health and Science University, Mail stop: L467 AD, 3181 SW Sam Jackson Park Rd, 97239, Portland, OR, USA aut NATIONALLICENCE 700 1- Davey Michael Oregon Health and Science University, Mail stop: L467 AD, 3181 SW Sam Jackson Park Rd, 97239, Portland, OR, USA aut NATIONALLICENCE 773 0- Inflammation Research SP Birkhäuser Verlag Basel 60/7(2011-07-01), 705-714 1023-3830 60:7<705 2011 60 11 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer