caa a22 4500 445885289 CHVBK 20180317145557.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s00011-011-0377-7 doi (NATIONALLICENCE)springer-10.1007/s00011-011-0377-7 Anti-inflammatory action of sulfated glucosamine on cytokine regulation in LPS-activated PMA-differentiated THP-1 macrophages [Elektronische Daten] [Jung-Ae Kim, Byul-Nim Ahn, Chang-Suk Kong, Se-Kwon Kim] Objective: The aim of this study was to evaluate the effect of sulfated glucosamine (SGlc) on the regulation of inflammatory cytokines and profiles involved in immunological activities. Changes in the inflammatory profiles of lipopolysaccharide (LPS)-activated phorbol 12-myristate 13-acetate (PMA)-differentiated THP-1 macrophage models were investigated following SGlc treatment. Methods: Human THP-1 macrophages were used to evaluate anti-inflammatory profiles. The cytokine secretion levels were measured by enzyme-linked immunosorbent assay (ELISA). Effects of SGlc on the regulation of mRNA and protein levels were determined using RT-PCR and Western blot analysis. The effect of SGlc on the activation of mitogen-activated protein kinases (MAPKs) and NF-κB protein was also determined by Western blot analysis. Results: Treatment of THP-1 cells with SGlc inhibited the production of pro-inflammatory cytokines such as tumor necrosis factor-α (TNF-α), interleukin (IL)-1β and IL-6. In addition, SGlc suppressed the mRNA and protein expression levels of inflammatory mediators such as inducible nitric oxide synthase, cyclooxygenase-2, TNF-α, IL-1β, IL-6, 5-lipoxygenase and cytoplasmic phospholipase A2 in LPS-activated THP-1 macrophages. Furthermore, we confirmed that the LPS-activated transcriptions of MAPKs and NF-κB were inhibited by SGlc treatment in PMA-differentiated THP-1 macrophages. Conclusion: These results suggest that SGlc can be considered as a potential anti-inflammatory supplement. Springer Basel AG, 2011 Sulfated glucosamine nationallicence THP-1 nationallicence Anti-inflammation nationallicence PMA nationallicence LPS nationallicence Cytokines nationallicence Kim Jung-Ae Department of Chemistry, Pukyong National University, 608-737, Busan, Republic of Korea aut Ahn Byul-Nim Department of Chemistry, Pukyong National University, 608-737, Busan, Republic of Korea aut Kong Chang-Suk Department of Food and Nutrition, College of Medical and Life Science, Silla University, 617-736, Busan, Republic of Korea aut Kim Se-Kwon Department of Chemistry, Pukyong National University, 608-737, Busan, Republic of Korea aut Inflammation Research SP Birkhäuser Verlag Basel 60/12(2011-12-01), 1131-1138 1023-3830 60:12<1131 2011 60 11 https://doi.org/10.1007/s00011-011-0377-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00011-011-0377-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kim Jung-Ae Department of Chemistry, Pukyong National University, 608-737, Busan, Republic of Korea aut NATIONALLICENCE 700 1- Ahn Byul-Nim Department of Chemistry, Pukyong National University, 608-737, Busan, Republic of Korea aut NATIONALLICENCE 700 1- Kong Chang-Suk Department of Food and Nutrition, College of Medical and Life Science, Silla University, 617-736, Busan, Republic of Korea aut NATIONALLICENCE 700 1- Kim Se-Kwon Department of Chemistry, Pukyong National University, 608-737, Busan, Republic of Korea aut NATIONALLICENCE 773 0- Inflammation Research SP Birkhäuser Verlag Basel 60/12(2011-12-01), 1131-1138 1023-3830 60:12<1131 2011 60 11 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer