caa a22 4500 445885297 CHVBK 20180317145557.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s00011-011-0379-5 doi (NATIONALLICENCE)springer-10.1007/s00011-011-0379-5 Systematic analysis of histamine and N -methylhistamine concentrations in organs from two common laboratory mouse strains: C57Bl/6 and Balb/c [Elektronische Daten] [Anna Zimmermann, Heike Burhenne, Volkhard Kaever, Roland Seifert, Detlef Neumann] Objective: Histamine plays a role in several (patho) physiological processes that are commonly studied in mouse models. However, a systematic quantification of histamine and its metabolite N-methylhistamine in mouse organs has not been reported so far. Methods: Balb/c and C57Bl/6 mice were grouped according to their sex and age. Brains, hearts, lungs, livers, kidneys, stomachs, intestines, thymi, spleens, and lymph nodes were excised, weighed, and homogenized. Histamine and N-methylhistamine were quantified simultaneously by a HPLC-mass spectrometry method. Results: In all organs analyzed, histamine and N-methylhistamine were detected; however, with quantitative differences. Histamine was present most abundantly in the stomach, lymph nodes, and thymus. The lowest histamine concentrations were detected in brain, liver, lung, and intestine. In most organs, the histamine concentrations increased age-dependently. Substantial concentrations of N-methylhistamine were detected only in lung, intestine and kidney, while in all other organs it was present only in minor quantities. Conclusion: HPLC-mass spectrometry is a useful method for the highly sensitive and simultaneous detection of histamine and N-methylhistamine. Histamine is present in virtually all organs, not only in those traditionally associated with histamine-mediated disease. Highest concentrations are found in stomach, lymph node, and thymus; medium concentrations in heart, spleen, and kidney; and lowest concentrations detected in intestine, brain, liver, and lung. Springer Basel AG, 2011 Histamine nationallicence N -Methylhistamine nationallicence Mouse nationallicence Mass spectrometry nationallicence Tissue concentration nationallicence Zimmermann Anna Institute of Pharmacology, Hannover Medical School, Carl-Neuberg-Str. 1, 30625, Hannover, Germany aut Burhenne Heike Institute of Pharmacology, Hannover Medical School, Carl-Neuberg-Str. 1, 30625, Hannover, Germany aut Kaever Volkhard Institute of Pharmacology, Hannover Medical School, Carl-Neuberg-Str. 1, 30625, Hannover, Germany aut Seifert Roland Institute of Pharmacology, Hannover Medical School, Carl-Neuberg-Str. 1, 30625, Hannover, Germany aut Neumann Detlef Institute of Pharmacology, Hannover Medical School, Carl-Neuberg-Str. 1, 30625, Hannover, Germany aut Inflammation Research SP Birkhäuser Verlag Basel 60/12(2011-12-01), 1153-1159 1023-3830 60:12<1153 2011 60 11 https://doi.org/10.1007/s00011-011-0379-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00011-011-0379-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zimmermann Anna Institute of Pharmacology, Hannover Medical School, Carl-Neuberg-Str. 1, 30625, Hannover, Germany aut NATIONALLICENCE 700 1- Burhenne Heike Institute of Pharmacology, Hannover Medical School, Carl-Neuberg-Str. 1, 30625, Hannover, Germany aut NATIONALLICENCE 700 1- Kaever Volkhard Institute of Pharmacology, Hannover Medical School, Carl-Neuberg-Str. 1, 30625, Hannover, Germany aut NATIONALLICENCE 700 1- Seifert Roland Institute of Pharmacology, Hannover Medical School, Carl-Neuberg-Str. 1, 30625, Hannover, Germany aut NATIONALLICENCE 700 1- Neumann Detlef Institute of Pharmacology, Hannover Medical School, Carl-Neuberg-Str. 1, 30625, Hannover, Germany aut NATIONALLICENCE 773 0- Inflammation Research SP Birkhäuser Verlag Basel 60/12(2011-12-01), 1153-1159 1023-3830 60:12<1153 2011 60 11 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer