caa a22 4500 445885335 CHVBK 20180317145557.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s00011-011-0381-y doi (NATIONALLICENCE)springer-10.1007/s00011-011-0381-y Vascular anti-inflammatory effects of curcumin on HMGB1-mediated responses in vitro [Elektronische Daten] [Dong-Chan Kim, Wonhwa Lee, Jong-Sup Bae] Aim and objective: High mobility group box 1 (HMGB1) protein up-regulates proinflammatory cytokines in several inflammatory diseases. Curcumin is a polyphenol responsible for the yellow color of the curry spice turmeric. It possesses diverse pharmacological properties such as anti-inflammatory, anti-oxidant, anti-proliferative and anti-angiogenic activities. However, the effects of curcumin on HMGB1-mediated proinflammatory responses have not been studied. Methods: The anti-inflammatory activities of curcumin were determined by measuring solute flux, leukocyte adhesion and migration and activation of proinflammatory proteins in HMGB1-activated human umbilical vein endothelial cells. Results: Curcumin inhibited the release of HMGB1 by lipopolysaccharide (LPS)- and HMGB1-mediated barrier disruption, neutrophil adhesion and migration, and expression of cell adhesion molecules. Further studies revealed that curcumin down-regulated the cell surface receptor of HMGB1 in human endothelial cells. Conclusion: These findings suggest that curcumin exerts anti-inflammatory effects in HMGB1-mediated proinflammatory responses, endorsing its usefulness as therapy for vascular inflammatory diseases. Springer Basel AG, 2011 Curcumin nationallicence HMGB1 nationallicence Endothelium nationallicence Inflammation nationallicence HMGB1 : High mobility group box 1 nationallicence HUVECs : Human umbilical vein endothelial cells nationallicence CAM : Cell adhesion molecule nationallicence VCAM : Vascular cell adhesion molecule nationallicence ICAM : Intracellular cell adhesion molecule nationallicence TEM : Transendothelial migration nationallicence LPS : Lipopolysaccharide nationallicence ECM : Extracellular cell adhesion molecule nationallicence Kim Dong-Chan Laboratory of Microvascular Circulation Research, NEUORNEX Inc., 711-823, Daegu, Republic of Korea aut Lee Wonhwa Department of Biochemistry and Cell Biology, School of Medicine, Daegu, Republic of Korea aut Bae Jong-Sup College of Pharmacy, Research Institute of Pharmaceutical Sciences, Kyungpook National University, 1370 Sankyuk-dong, Buk-gu, 702-701, Daegu, Republic of Korea aut Inflammation Research SP Birkhäuser Verlag Basel 60/12(2011-12-01), 1161-1168 1023-3830 60:12<1161 2011 60 11 https://doi.org/10.1007/s00011-011-0381-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00011-011-0381-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kim Dong-Chan Laboratory of Microvascular Circulation Research, NEUORNEX Inc., 711-823, Daegu, Republic of Korea aut NATIONALLICENCE 700 1- Lee Wonhwa Department of Biochemistry and Cell Biology, School of Medicine, Daegu, Republic of Korea aut NATIONALLICENCE 700 1- Bae Jong-Sup College of Pharmacy, Research Institute of Pharmaceutical Sciences, Kyungpook National University, 1370 Sankyuk-dong, Buk-gu, 702-701, Daegu, Republic of Korea aut NATIONALLICENCE 773 0- Inflammation Research SP Birkhäuser Verlag Basel 60/12(2011-12-01), 1161-1168 1023-3830 60:12<1161 2011 60 11 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer