caa a22 4500 445885378 CHVBK 20180317145557.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s00011-011-0378-6 doi (NATIONALLICENCE)springer-10.1007/s00011-011-0378-6 HMC-1 human mast cells synthesize neurotensin (NT) precursor, secrete bioactive NT-like peptide(s) and express NT receptor NTS1 [Elektronische Daten] [David Cochrane, Robert Carraway, Kimberly Harrington, Melissa Laudano, Stephen Rawlings, Ross Feldberg] Objective and design: To determine if mast cells synthesize the inflammatory peptide, neurotensin (NT), secrete immunoreactive and bioactive NT, and express the NT receptor NTS1. Materials: HMC-1 cells, pleural mast cells from Sprague-Dawley rats, LAD2 mast cells, and human cord blood mast cells were used. Treatment: HMC-1 cells were stimulated with NT, C48/80, mastoparan, or PGE2. For changes in cutaneous vascular permeability, anesthetized rats were injected intravenously with Evans Blue dye and intradermally with saline, NT, histamine, diphenhydramine, and C48/80. Methods: RT-PCR was used to identify RNA transcripts. Histamine was measured by fluorometric assay. In vivo cutaneous vascular permeability assays, radio-immunoassays for NT, Western blotting for the NT precursor protein and NTS1 protein from HMC-1 cells and tissues from rats were used. Immunohistochemistry was used to identify NT precursor-like proteins in HMC-1 mast cells. Results: HMC-1 cells express mRNAs for NT precursor, PC5A processing enzyme and NTS1 receptor. Human cord blood mast cells and LAD2 mast cells express mRNA transcripts for NT precursor and NTS1. Western blotting showed NT precursor and NTS1 receptor in HMC1. Rat tissues with high numbers of mast cells contained NT precursor proteins. NT-like peptides from HMC-1 displayed NT-like bioactivity. Conclusions: HMC-1 mast cells synthesize and secrete immunoreactive and bioactive NT-like peptide(s) and express the NT receptor, suggesting that NT from mast cells might serve autocrine and paracrine roles. Springer Basel AG, 2011 HMC-1 mast cell nationallicence Neurotensin nationallicence Neurotensin receptor nationallicence Tumor growth nationallicence Cochrane David Department of Biology, Tufts University, 02155, Medford, MA, USA aut Carraway Robert Department of Microbiology and Physiological Systems, University of Massachusetts Medical School, 55 Lake Ave. North, 01655, Worcester, MA, USA aut Harrington Kimberly Department of Biology, Tufts University, 02155, Medford, MA, USA aut Laudano Melissa Department of Biology, Tufts University, 02155, Medford, MA, USA aut Rawlings Stephen Department of Biology, Tufts University, 02155, Medford, MA, USA aut Feldberg Ross Department of Biology, Tufts University, 02155, Medford, MA, USA aut Inflammation Research SP Birkhäuser Verlag Basel 60/12(2011-12-01), 1139-1151 1023-3830 60:12<1139 2011 60 11 https://doi.org/10.1007/s00011-011-0378-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00011-011-0378-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cochrane David Department of Biology, Tufts University, 02155, Medford, MA, USA aut NATIONALLICENCE 700 1- Carraway Robert Department of Microbiology and Physiological Systems, University of Massachusetts Medical School, 55 Lake Ave. North, 01655, Worcester, MA, USA aut NATIONALLICENCE 700 1- Harrington Kimberly Department of Biology, Tufts University, 02155, Medford, MA, USA aut NATIONALLICENCE 700 1- Laudano Melissa Department of Biology, Tufts University, 02155, Medford, MA, USA aut NATIONALLICENCE 700 1- Rawlings Stephen Department of Biology, Tufts University, 02155, Medford, MA, USA aut NATIONALLICENCE 700 1- Feldberg Ross Department of Biology, Tufts University, 02155, Medford, MA, USA aut NATIONALLICENCE 773 0- Inflammation Research SP Birkhäuser Verlag Basel 60/12(2011-12-01), 1139-1151 1023-3830 60:12<1139 2011 60 11 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer