caa a22 4500 445885386 CHVBK 20180317145557.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s00011-011-0376-8 doi (NATIONALLICENCE)springer-10.1007/s00011-011-0376-8 The involvement of the HO-1 pathway in the anti-inflammatory action of a sulfated polysaccharide isolated from the red seaweed Gracilaria birdiae [Elektronische Daten] [Edfranck de Sousa Oliveira Vanderlei, Ianna de Araújo, Ana Quinderé, Bruno Fontes, Ygor Eloy, José Rodrigues, Antonio Silva, Hellíada Chaves, Roberta Jorge, Dalgimar de Menezes, Janaína Evangelista, Mirna Bezerra, Norma Benevides] Objectives: The aim of this study was to investigate the involvement of the hemoxigenase-1 (HO-1) pathway in the anti-inflammatory action of a sulfated polysaccharide from the red seaweed Gracilaria birdiae (SP-Gb). Methods: SP-Gb (5, 10 and 20mg/kg) was administered to Wistar rats in a peritonitis model using carrageenan or a paw edema model using carrageenan or dextran. To analyze the involvement of HO-1 in the anti-inflammatory activity of SP-Gb, the animals were pretreated subcutaneously with a specific HO-1 inhibitor (ZnPP IX). To evaluate the systemic effects, SP-Gb (10mg/kg) was administered to mice intraperitoneally before waiting for 48h or for 14days. Results: SP-Gb (10mg/kg) caused an anti-inflammatory effect that was evidenced by a decrease in leukocytes in the peritoneal cavity. SP-Gb also reduced the paw edema induced by carrageenan and inhibited the paw edema induced by dextran in the first half-hour. After being inhibited by ZnPP IX, the anti-inflammatory effect of SP-Gb on carrageenan-induced rat paw edema was not observed. SP-Gb did not cause mortality or significant changes in the biochemical, hematological and histopathological parameters. Conclusion: SP-Gb may be used as a tool for further investigations into the inflammatory processes associated with the hemoxigenase-1 pathway. Springer Basel AG, 2011 Seaweed nationallicence Sulfated polysaccharide nationallicence Gracilaria birdiae nationallicence Inflammation nationallicence de Sousa Oliveira Vanderlei Edfranck Department of Biochemistry, Molecular Biology, Federal University of Ceará, Campus do Pici, Bloco 907, 60455-760, Fortaleza, CE, Brazil aut de Araújo Ianna Department of Biochemistry, Molecular Biology, Federal University of Ceará, Campus do Pici, Bloco 907, 60455-760, Fortaleza, CE, Brazil aut Quinderé Ana Department of Biochemistry, Molecular Biology, Federal University of Ceará, Campus do Pici, Bloco 907, 60455-760, Fortaleza, CE, Brazil aut Fontes Bruno Department of Biochemistry, Molecular Biology, Federal University of Ceará, Campus do Pici, Bloco 907, 60455-760, Fortaleza, CE, Brazil aut Eloy Ygor Department of Biochemistry, Molecular Biology, Federal University of Ceará, Campus do Pici, Bloco 907, 60455-760, Fortaleza, CE, Brazil aut Rodrigues José Department of Biochemistry, Molecular Biology, Federal University of Ceará, Campus do Pici, Bloco 907, 60455-760, Fortaleza, CE, Brazil aut Silva Antonio Faculty of Dentistry, Federal University of Ceará, Sobral, CE, Brazil aut Chaves Hellíada Faculty of Dentistry, Federal University of Ceará, Sobral, CE, Brazil aut Jorge Roberta Department of Physiology and Pharmacology, Federal University of Ceará, Fortaleza, CE, Brazil aut de Menezes Dalgimar Department of Pathology and Forensic Medicine, Federal University of Ceará, Fortaleza, CE, Brazil aut Evangelista Janaína Faculty of Veterinary Medicine, State University of Ceará, Fortaleza, CE, Brazil aut Bezerra Mirna Faculty of Medicine, Federal University of Ceará, Sobral, CE, Brazil aut Benevides Norma Department of Biochemistry, Molecular Biology, Federal University of Ceará, Campus do Pici, Bloco 907, 60455-760, Fortaleza, CE, Brazil aut Inflammation Research SP Birkhäuser Verlag Basel 60/12(2011-12-01), 1121-1130 1023-3830 60:12<1121 2011 60 11 https://doi.org/10.1007/s00011-011-0376-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00011-011-0376-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- de Sousa Oliveira Vanderlei Edfranck Department of Biochemistry, Molecular Biology, Federal University of Ceará, Campus do Pici, Bloco 907, 60455-760, Fortaleza, CE, Brazil aut NATIONALLICENCE 700 1- de Araújo Ianna Department of Biochemistry, Molecular Biology, Federal University of Ceará, Campus do Pici, Bloco 907, 60455-760, Fortaleza, CE, Brazil aut NATIONALLICENCE 700 1- Quinderé Ana Department of Biochemistry, Molecular Biology, Federal University of Ceará, Campus do Pici, Bloco 907, 60455-760, Fortaleza, CE, Brazil aut NATIONALLICENCE 700 1- Fontes Bruno Department of Biochemistry, Molecular Biology, Federal University of Ceará, Campus do Pici, Bloco 907, 60455-760, Fortaleza, CE, Brazil aut NATIONALLICENCE 700 1- Eloy Ygor Department of Biochemistry, Molecular Biology, Federal University of Ceará, Campus do Pici, Bloco 907, 60455-760, Fortaleza, CE, Brazil aut NATIONALLICENCE 700 1- Rodrigues José Department of Biochemistry, Molecular Biology, Federal University of Ceará, Campus do Pici, Bloco 907, 60455-760, Fortaleza, CE, Brazil aut NATIONALLICENCE 700 1- Silva Antonio Faculty of Dentistry, Federal University of Ceará, Sobral, CE, Brazil aut NATIONALLICENCE 700 1- Chaves Hellíada Faculty of Dentistry, Federal University of Ceará, Sobral, CE, Brazil aut NATIONALLICENCE 700 1- Jorge Roberta Department of Physiology and Pharmacology, Federal University of Ceará, Fortaleza, CE, Brazil aut NATIONALLICENCE 700 1- de Menezes Dalgimar Department of Pathology and Forensic Medicine, Federal University of Ceará, Fortaleza, CE, Brazil aut NATIONALLICENCE 700 1- Evangelista Janaína Faculty of Veterinary Medicine, State University of Ceará, Fortaleza, CE, Brazil aut NATIONALLICENCE 700 1- Bezerra Mirna Faculty of Medicine, Federal University of Ceará, Sobral, CE, Brazil aut NATIONALLICENCE 700 1- Benevides Norma Department of Biochemistry, Molecular Biology, Federal University of Ceará, Campus do Pici, Bloco 907, 60455-760, Fortaleza, CE, Brazil aut NATIONALLICENCE 773 0- Inflammation Research SP Birkhäuser Verlag Basel 60/12(2011-12-01), 1121-1130 1023-3830 60:12<1121 2011 60 11 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer