caa a22 4500 445885394 CHVBK 20180317145557.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s00011-010-0260-y doi (NATIONALLICENCE)springer-10.1007/s00011-010-0260-y Comparison of lung accumulation of cationic liposomes in normal rats and LPS-treated rats [Elektronische Daten] [Susanne Herber-Jonat, Rashmi Mittal, Stefan Gsinn, Hermann Bohnenkamp, Eric Guenzi, Andreas Schulze] Objective: Cationic liposomes have been shown to target angiogenic endothelial cells in lungs and joints with evidence of chronic inflammation. We sought to determine whether cationic liposomes accumulate in acutely inflamed lung tissue. Subjects, treatment and methods: Acute lung injury was induced by intratracheal instillation of lipopolysaccharide (LPS) in Sprague Dawley rats. The controls received saline. Following instillation, the rats were ventilated for 5h. Four hours after LPS-instillation each rat received rhodamine-labeled, cationic liposomes intravenously. The liposomes were allowed to circulate for 1h. Thereafter, a bronchoalveolar lavage (BAL) was done and the lungs were perfused with saline and formalin. Accumulation of liposomes was assessed by quantitative confocal microscopy and determination of rhodamine-content in lung tissue. Results: LPS induced a significant increase in BAL white blood cell count (3,444±1,420 vs. 1,314±906*103/μl) and cytokines (IL-1β: 145.57 vs. 51.94pg/ml; TNF-α: 3,467.5 vs. 42.1pg/ml) as compared to controls. Cationic liposomes exhibited an accumulation up to twofold in the inflamed lung tissue as compared to healthy lungs (fluorescent pixels 2.93 vs. 1.90(%)). Conclusions: Our findings indicate that cationic liposomes accumulate in the acutely inflamed lung tissue. This uptake raises the possibility of using cationic liposomes to direct diagnostic/therapeutic agents selectively to the sites of acute inflammation in the lung. Springer Basel AG, 2010 Drug delivery systems nationallicence Drug targeting nationallicence Liposomes nationallicence Acute lung injury nationallicence Endothelial cells nationallicence ALI : Acute lung injury nationallicence ARDS : Acute respiratory distress syndrome nationallicence BAL : Bronchoalveolar lavage nationallicence LPS : Lipopolysaccharide from E. coli nationallicence Herber-Jonat Susanne Division of Neonatology, University Children's Hospital, Perinatal Center, Ludwig-Maximilian-University Munich, Grosshadern, Marchioninistr.15, 81377, Munich, Germany aut Mittal Rashmi Division of Neonatology, University Children's Hospital, Perinatal Center, Ludwig-Maximilian-University Munich, Grosshadern, Marchioninistr.15, 81377, Munich, Germany aut Gsinn Stefan Division of Neonatology, University Children's Hospital, Perinatal Center, Ludwig-Maximilian-University Munich, Grosshadern, Marchioninistr.15, 81377, Munich, Germany aut Bohnenkamp Hermann Division of Research, MediGene AG, Martinsried, Germany aut Guenzi Eric Division of Research, MediGene AG, Martinsried, Germany aut Schulze Andreas Division of Neonatology, University Children's Hospital, Perinatal Center, Ludwig-Maximilian-University Munich, Grosshadern, Marchioninistr.15, 81377, Munich, Germany aut Inflammation Research SP Birkhäuser Verlag Basel 60/3(2011-03-01), 245-253 1023-3830 60:3<245 2011 60 11 https://doi.org/10.1007/s00011-010-0260-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00011-010-0260-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Herber-Jonat Susanne Division of Neonatology, University Children's Hospital, Perinatal Center, Ludwig-Maximilian-University Munich, Grosshadern, Marchioninistr.15, 81377, Munich, Germany aut NATIONALLICENCE 700 1- Mittal Rashmi Division of Neonatology, University Children's Hospital, Perinatal Center, Ludwig-Maximilian-University Munich, Grosshadern, Marchioninistr.15, 81377, Munich, Germany aut NATIONALLICENCE 700 1- Gsinn Stefan Division of Neonatology, University Children's Hospital, Perinatal Center, Ludwig-Maximilian-University Munich, Grosshadern, Marchioninistr.15, 81377, Munich, Germany aut NATIONALLICENCE 700 1- Bohnenkamp Hermann Division of Research, MediGene AG, Martinsried, Germany aut NATIONALLICENCE 700 1- Guenzi Eric Division of Research, MediGene AG, Martinsried, Germany aut NATIONALLICENCE 700 1- Schulze Andreas Division of Neonatology, University Children's Hospital, Perinatal Center, Ludwig-Maximilian-University Munich, Grosshadern, Marchioninistr.15, 81377, Munich, Germany aut NATIONALLICENCE 773 0- Inflammation Research SP Birkhäuser Verlag Basel 60/3(2011-03-01), 245-253 1023-3830 60:3<245 2011 60 11 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer