caa a22 4500 445885416 CHVBK 20180317145558.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s00011-010-0268-3 doi (NATIONALLICENCE)springer-10.1007/s00011-010-0268-3 MnTMPyP, a superoxide dismutase/catalase mimetic, decreases inflammatory indices in ischemic acute kidney injury [Elektronische Daten] [Jordan Mortensen, Brian Shames, Christopher Johnson, Vani Nilakantan] Objective: This study investigates the effect of a superoxide dismutase mimetic, MnTMPyP, on pro- and anti-inflammatory cytokines in acute renal ischemia-reperfusion (IR). Materials and treatment: Male Sprague-Dawley rats underwent bilateral clamping of the renal arteries for 45min followed by 1, 4, or 24h of reperfusion. A subset of animals was treated with MnTMPyP (5mg/kg, i.p.) or saline. Porcine proximal tubular epithelial cells were ATP-depleted for 4h followed by recovery for 2h. Methods: Cytokines were analyzed by ELISA, and ED1+ macrophages and CD8+ T lymphocytes by immunohistochemistry. Statistical analysis was performed using ANOVA. Results: MnTMPyP attenuated the IR-mediated increase in serum creatinine and circulating levels of interleukin (IL)-2 following 24h of reperfusion. Furthermore, treatment attenuated increases in tissue levels of tumor necrosis factor (TNF)-α, IL-2, IL-4, and IL-13. MnTMPyP partially prevented the IR-induced infiltration of ED1+ macrophages and CD8+ T lymphocytes in the kidney. ATP depletion-recovery of porcine proximal tubular epithelial cells resulted in decreased IL-6 and IL-10 levels, and MnTMPyP partially restored these cytokines. Conclusions: These results show that MnTMPyP is partially effective in reducing inflammation associated with renal IR and that reactive oxygen species play a role in modulating both pro- and anti-inflammatory pathways in acute kidney injury. Springer Basel AG, 2010 Ischemia-reperfusion nationallicence Kidney nationallicence Reactive oxygen nationallicence Inflammatory cytokines nationallicence TNF nationallicence MnTMPyP nationallicence Mortensen Jordan Division of Transplant Surgery, Medical College of Wisconsin, 8701 Watertown Plank Rd, 53226, Milwaukee, WI, USA aut Shames Brian Division of Transplant Surgery, Medical College of Wisconsin, 8701 Watertown Plank Rd, 53226, Milwaukee, WI, USA aut Johnson Christopher Division of Transplant Surgery, Medical College of Wisconsin, 8701 Watertown Plank Rd, 53226, Milwaukee, WI, USA aut Nilakantan Vani Division of Transplant Surgery, Medical College of Wisconsin, 8701 Watertown Plank Rd, 53226, Milwaukee, WI, USA aut Inflammation Research SP Birkhäuser Verlag Basel 60/3(2011-03-01), 299-307 1023-3830 60:3<299 2011 60 11 https://doi.org/10.1007/s00011-010-0268-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00011-010-0268-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mortensen Jordan Division of Transplant Surgery, Medical College of Wisconsin, 8701 Watertown Plank Rd, 53226, Milwaukee, WI, USA aut NATIONALLICENCE 700 1- Shames Brian Division of Transplant Surgery, Medical College of Wisconsin, 8701 Watertown Plank Rd, 53226, Milwaukee, WI, USA aut NATIONALLICENCE 700 1- Johnson Christopher Division of Transplant Surgery, Medical College of Wisconsin, 8701 Watertown Plank Rd, 53226, Milwaukee, WI, USA aut NATIONALLICENCE 700 1- Nilakantan Vani Division of Transplant Surgery, Medical College of Wisconsin, 8701 Watertown Plank Rd, 53226, Milwaukee, WI, USA aut NATIONALLICENCE 773 0- Inflammation Research SP Birkhäuser Verlag Basel 60/3(2011-03-01), 299-307 1023-3830 60:3<299 2011 60 11 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer