caa a22 4500 445885424 CHVBK 20180317145558.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s00011-010-0264-7 doi (NATIONALLICENCE)springer-10.1007/s00011-010-0264-7 Blood lymphocyte blastogenesis in patients with thyroid dysfunction: exvivo response to mitogen activation and cyclosporinA [Elektronische Daten] [Garyphallia Papaioannou, Fotios Michelis, Konstantinos Papamichael, Helen Karga, Ekaterini Tiligada] Objective: To evaluate lymphocyte activation following mitogen and cyclosporinA (CsA) administration in peripheral blood of hyperthyroxinaemic and hypothyroid patients. Materials and methods: Lymphocyte activation was evaluated by determining blastogenesis in 48h cultured blood lymphocytes obtained from eight hyperthyroxinaemic and eight hypothyroid patients, following phytohaemagglutinin (PHA)-induced stimulation in the absence or presence of CsA. Twelve healthy volunteers served as controls. Results and conclusions: Lymphocytes from hypothyroid patients exhibited reduced response to PHA and lower sensitivity to CsA compared with control, which could be attributed to their reduced activation capability coexisting with hypothyroidism. In hyperthyroxinaemic samples, the actions of high CsA concentrations were mostly targeted toward activated lymphoblasts. Considering the cellular targets that thyroid hormones and CsA may share, the therapeutic implications of their cross-talk need careful consideration. Springer Basel AG, 2010 Blastogenesis nationallicence Cyclosporine nationallicence Lymphocytes nationallicence Patients nationallicence Thyroid hormones nationallicence Papaioannou Garyphallia Department of Pharmacology, Medical School, University of Athens, M. Asias 75, 11527, Athens, Greece aut Michelis Fotios Department of Pharmacology, Medical School, University of Athens, M. Asias 75, 11527, Athens, Greece aut Papamichael Konstantinos Department of Pharmacology, Medical School, University of Athens, M. Asias 75, 11527, Athens, Greece aut Karga Helen Second Division of Endocrinology, Alexandra Hospital, Athens, Greece aut Tiligada Ekaterini Department of Pharmacology, Medical School, University of Athens, M. Asias 75, 11527, Athens, Greece aut Inflammation Research SP Birkhäuser Verlag Basel 60/3(2011-03-01), 265-270 1023-3830 60:3<265 2011 60 11 https://doi.org/10.1007/s00011-010-0264-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00011-010-0264-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Papaioannou Garyphallia Department of Pharmacology, Medical School, University of Athens, M. Asias 75, 11527, Athens, Greece aut NATIONALLICENCE 700 1- Michelis Fotios Department of Pharmacology, Medical School, University of Athens, M. Asias 75, 11527, Athens, Greece aut NATIONALLICENCE 700 1- Papamichael Konstantinos Department of Pharmacology, Medical School, University of Athens, M. Asias 75, 11527, Athens, Greece aut NATIONALLICENCE 700 1- Karga Helen Second Division of Endocrinology, Alexandra Hospital, Athens, Greece aut NATIONALLICENCE 700 1- Tiligada Ekaterini Department of Pharmacology, Medical School, University of Athens, M. Asias 75, 11527, Athens, Greece aut NATIONALLICENCE 773 0- Inflammation Research SP Birkhäuser Verlag Basel 60/3(2011-03-01), 265-270 1023-3830 60:3<265 2011 60 11 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer