caa a22 4500 445885440 CHVBK 20180317145558.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s00011-010-0259-4 doi (NATIONALLICENCE)springer-10.1007/s00011-010-0259-4 Interleukin-10 repletion suppresses pro-inflammatory cytokines and decreases liver pathology without altering viral replication in murine cytomegalovirus (MCMV)-infected IL-10 knockout mice [Elektronische Daten] [Yajarayma Tang-Feldman, G. Lochhead, Stephanie Lochhead, Cindy Yu, Claire Pomeroy] Objective and design: To determine the role of interleukin-10 (IL-10) in protecting against the deleterious pro-inflammatory cytokine response to murine cytomegalovirus (MCMV), we studied the impact of IL-10 repletion in MCMV-infected IL-10 knockout (KO) mice. Materials and methods: IL-10 KO mice were infected with a sub-lethal dose of MCMV and treated daily with 5μg of mouse recombinant IL-10 (mrIL-10). Cytokine transcription, viral load, cytokine expression and liver histopathology were assessed in IL-10 treated and untreated mice. Results: mrIL-10 repletion suppressed the exaggerated pro-inflammatory cytokine response observed in IL-10 KO mice (vs. control) both systemically and at the organ level, without affecting viral load. Levels of IFN-γ and TNF-α mRNA in livers of treated mice were ~50-70-fold lower than in untreated mice at day 5 post-infection (p≤0.05). In spleens and sera, levels of IFN-γ and IL-6 were significantly lower in treated mice than in untreated mice at day 5-7 post-infection (p≤0.05). IL-10 blunting of cytokine responses was accompanied by attenuation of inflammation in livers of treated mice. Conclusions: Repletion of IL-10 modulates the exaggerated pro-inflammatory cytokine responses that characterize IL-10 KO mice and protects against liver damage without altering viral load. IL-10 may be useful to control dysregulated pro-inflammatory cytokines responses during CMV infection. The Author(s), 2010 MCMV nationallicence Pro-inflammatory cytokines nationallicence IL-10 nationallicence IL-10 deficient nationallicence Repletion nationallicence Viral load nationallicence Tang-Feldman Yajarayma Division of Infectious Diseases, Department of Internal Medicine, University of California, Davis Health System, 4150V St., Suite 500, 95817, Sacramento, CA, USA aut Lochhead G. Division of Infectious Diseases, Department of Internal Medicine, University of California, Davis Health System, 4150V St., Suite 500, 95817, Sacramento, CA, USA aut Lochhead Stephanie Division of Infectious Diseases, Department of Internal Medicine, University of California, Davis Health System, 4150V St., Suite 500, 95817, Sacramento, CA, USA aut Yu Cindy Medical Pathology, Davis Health System, University of California, 95817, Sacramento, CA, USA aut Pomeroy Claire Division of Infectious Diseases, Department of Internal Medicine, University of California, Davis Health System, 4150V St., Suite 500, 95817, Sacramento, CA, USA aut Inflammation Research SP Birkhäuser Verlag Basel 60/3(2011-03-01), 233-243 1023-3830 60:3<233 2011 60 11 https://doi.org/10.1007/s00011-010-0259-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00011-010-0259-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tang-Feldman Yajarayma Division of Infectious Diseases, Department of Internal Medicine, University of California, Davis Health System, 4150V St., Suite 500, 95817, Sacramento, CA, USA aut NATIONALLICENCE 700 1- Lochhead G. Division of Infectious Diseases, Department of Internal Medicine, University of California, Davis Health System, 4150V St., Suite 500, 95817, Sacramento, CA, USA aut NATIONALLICENCE 700 1- Lochhead Stephanie Division of Infectious Diseases, Department of Internal Medicine, University of California, Davis Health System, 4150V St., Suite 500, 95817, Sacramento, CA, USA aut NATIONALLICENCE 700 1- Yu Cindy Medical Pathology, Davis Health System, University of California, 95817, Sacramento, CA, USA aut NATIONALLICENCE 700 1- Pomeroy Claire Division of Infectious Diseases, Department of Internal Medicine, University of California, Davis Health System, 4150V St., Suite 500, 95817, Sacramento, CA, USA aut NATIONALLICENCE 773 0- Inflammation Research SP Birkhäuser Verlag Basel 60/3(2011-03-01), 233-243 1023-3830 60:3<233 2011 60 11 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer