caa a22 4500 445885459 CHVBK 20180317145558.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s00011-010-0258-5 doi (NATIONALLICENCE)springer-10.1007/s00011-010-0258-5 The modulatory effect of lipids and glucose on the neonatal immune response induced by Staphylococcus epidermidis [Elektronische Daten] [Berit Haase, Kirstin Faust, Mathias Heidemann, Tasja Scholz, Martin Demmert, Birte Tröger, Alexander Herz, Christoph Härtel] Background: Parenteral nutrition is an important risk factor for late onset sepsis in neonates. This may be caused by the long-term need of central venous access but also through a potentially modulating effect of lipids and glucose on the immune function. Objective: It was the aim of this study to characterize the effect of lipids and glucose on the neonatal immune response in an in vitro Staphylococcus epidermidis sepsis model using whole cord blood of healthy term infants and preterm infants. Results: At the single cell level, IL-6, IL-8 and TNF-α expression of CD14+ cells was significantly increased upon addition of 1% lipids, while the addition of clinically meaningful lipid concentrations had no remarkable effect. When glucose was added to whole cord blood cultures, a dose-dependent effect was demonstrated for IL-8 expression but not for other cytokines. Conclusions: These in vitro data suggest that the pro-inflammatory cytokine response to S. epidermidis may be modulated by lipids and glucose. Further studies are needed to investigate whether these findings are applicable to clinical settings and to evaluate the role of cytokine monitoring in infants receiving long-term parenteral nutrition. Springer Basel AG, 2010 Staphylococcus epidermidis nationallicence Intravenous lipids nationallicence Glucose nationallicence Cytokine response nationallicence IL : Interleukin nationallicence TNF : Tumor necrosis factor nationallicence S. epidermidis : Staphylococcus epidermidis nationallicence Haase Berit Department of Paediatrics, University Hospital Schleswig-Holstein, University of Lübeck Medical School, Ratzeburger Allee 160, Campus Lübeck, 23538, Lübeck, Germany aut Faust Kirstin Department of Paediatrics, University Hospital Schleswig-Holstein, University of Lübeck Medical School, Ratzeburger Allee 160, Campus Lübeck, 23538, Lübeck, Germany aut Heidemann Mathias Department of Paediatrics, University Hospital Schleswig-Holstein, University of Lübeck Medical School, Ratzeburger Allee 160, Campus Lübeck, 23538, Lübeck, Germany aut Scholz Tasja Department of Paediatrics, University Hospital Schleswig-Holstein, University of Lübeck Medical School, Ratzeburger Allee 160, Campus Lübeck, 23538, Lübeck, Germany aut Demmert Martin Department of Paediatrics, University Hospital Schleswig-Holstein, University of Lübeck Medical School, Ratzeburger Allee 160, Campus Lübeck, 23538, Lübeck, Germany aut Tröger Birte Department of Paediatrics, University Hospital Schleswig-Holstein, University of Lübeck Medical School, Ratzeburger Allee 160, Campus Lübeck, 23538, Lübeck, Germany aut Herz Alexander Department of Paediatrics, University Hospital Schleswig-Holstein, University of Lübeck Medical School, Ratzeburger Allee 160, Campus Lübeck, 23538, Lübeck, Germany aut Härtel Christoph Department of Paediatrics, University Hospital Schleswig-Holstein, University of Lübeck Medical School, Ratzeburger Allee 160, Campus Lübeck, 23538, Lübeck, Germany aut Inflammation Research SP Birkhäuser Verlag Basel 60/3(2011-03-01), 227-232 1023-3830 60:3<227 2011 60 11 https://doi.org/10.1007/s00011-010-0258-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00011-010-0258-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Haase Berit Department of Paediatrics, University Hospital Schleswig-Holstein, University of Lübeck Medical School, Ratzeburger Allee 160, Campus Lübeck, 23538, Lübeck, Germany aut NATIONALLICENCE 700 1- Faust Kirstin Department of Paediatrics, University Hospital Schleswig-Holstein, University of Lübeck Medical School, Ratzeburger Allee 160, Campus Lübeck, 23538, Lübeck, Germany aut NATIONALLICENCE 700 1- Heidemann Mathias Department of Paediatrics, University Hospital Schleswig-Holstein, University of Lübeck Medical School, Ratzeburger Allee 160, Campus Lübeck, 23538, Lübeck, Germany aut NATIONALLICENCE 700 1- Scholz Tasja Department of Paediatrics, University Hospital Schleswig-Holstein, University of Lübeck Medical School, Ratzeburger Allee 160, Campus Lübeck, 23538, Lübeck, Germany aut NATIONALLICENCE 700 1- Demmert Martin Department of Paediatrics, University Hospital Schleswig-Holstein, University of Lübeck Medical School, Ratzeburger Allee 160, Campus Lübeck, 23538, Lübeck, Germany aut NATIONALLICENCE 700 1- Tröger Birte Department of Paediatrics, University Hospital Schleswig-Holstein, University of Lübeck Medical School, Ratzeburger Allee 160, Campus Lübeck, 23538, Lübeck, Germany aut NATIONALLICENCE 700 1- Herz Alexander Department of Paediatrics, University Hospital Schleswig-Holstein, University of Lübeck Medical School, Ratzeburger Allee 160, Campus Lübeck, 23538, Lübeck, Germany aut NATIONALLICENCE 700 1- Härtel Christoph Department of Paediatrics, University Hospital Schleswig-Holstein, University of Lübeck Medical School, Ratzeburger Allee 160, Campus Lübeck, 23538, Lübeck, Germany aut NATIONALLICENCE 773 0- Inflammation Research SP Birkhäuser Verlag Basel 60/3(2011-03-01), 227-232 1023-3830 60:3<227 2011 60 11 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer